MAP3K10 (NM_002446) Human Tagged ORF Clone
CAT#: RC218669
- TrueORF®
MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10)
ORF Plasmid: tGFP
"NM_002446" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 10,480.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MEKK10; MLK2; MST |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218669 representing NM_002446
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGAGGAGGAGGAGGGGGCGGTGGCCAAGGAGTGGGGCACGACCCCCGCGGGGCCCGTCTGGACCGCGG TGTTCGACTACGAGGCGGCGGGCGACGAGGAGCTGACCCTGCGGAGGGGCGATCGCGTCCAGGTGCTTTC CCAAGACTGTGCGGTGTCCGGCGACGAGGGCTGGTGGACCGGGCAGCTCCCCAGCGGCCGCGTGGGCGTC TTCCCCAGCAACTACGTGGCCCCCGGCGCCCCCGCTGCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCC CCTTCCACGAGCTGCAGCTAGAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTG GCGTGGCGAGGAGGTGGCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAG CAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTTAGGGGCGCCT GCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGTGCACTGAGCAGGGTGCTGGC AGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCTGTGCAGGTGGCCCGGGGCATGAACTACCTA CACAATGATGCCCCTGTGCCCATCATCCACCGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCA TCGAGAACCACAACCTCGCAGACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAA GACCACCAAGATGAGCGCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTC TCCAAAAGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAGGTCCCCTACC GTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTGACGCTGCCCATTCCCTCCAC GTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGGGACCCAGACCCCCACGGGCGGCCAGATTTC GGTAGCATCTTGAAGCGGCTTGAAGTCATCGAACAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCC ACTCGCTGCAGGAAGACTGGAAGCTGGAGATTCAGCACATGTTTGATGACCTTCGGACCAAGGAGAAGGA GCTTCGGAGCCGTGAGGAGGAGCTGCTGCGGGCGGCACAGGAGCAGCGCTTCCAGGAGGAGCAGCTGCGG CGGCGGGAGCAGGAGCTGGCAGAACGTGAGATGGACATCGTGGAACGGGAGCTGCACCTGCTCATGTGCC AGCTGAGCCAGGAGAAGCCCCGGGTCCGCAAGCGCAAGGGCAACTTCAAGCGCAGCCGCCTGCTCAAGCT GCGGGAAGGCGGCAGCCACATCAGCCTGCCCTCTGGCTTTGAGCATAAGATCACAGTCCAGGCCTCTCCA ACTCTGGATAAGCGGAAAGGATCCGATGGGGCCAGCCCCCCTGCAAGCCCCAGCATCATCCCCCGGCTGA GGGCCATTCGCCTGACTCCCGTGGACTGTGGTGGCAGCAGCAGTGGCAGCAGCAGTGGAGGAAGTGGGAC ATGGAGCCGCGGTGGGCCCCCAAAGAAGGAAGAACTGGTCGGGGGCAAGAAGAAGGGACGAACGTGGGGG CCCAGCTCCACCCTGCAGAAGGAGCGGGTGGGAGGAGAGGAGAGGCTGAAGGGGCTGGGGGAAGGAAGCA AACAGTGGTCATCAAGTGCCCCCAACCTGGGCAAGTCCCCCAAACACACACCCATCGCCCCTGGCTTTGC CAGCCTCAATGAGATGGAGGAGTTCGCGGAGGCAGAGGATGGAGGCAGCAGCGTGCCCCCTTCCCCCTAC TCGACCCCGTCCTACCTCTCAGTGCCACTGCCTGCCGAGCCCTCCCCGGGGGCGCGGGCGCCGTGGGAGC CGACGCCGTCCGCGCCCCCCGCTCGGTGGGGACACGGCGCCCGGCGGCGCTGCGACCTGGCGCTGCTAGG CTGCGCCACGCTGCTGGGGGCTGTGGGCCTGGGCGCCGACGTGGCCGAGGCGCGCGCGGCCGACGGTGAG GAGCAGCGGCGCTGGCTCGACGGCCTCTTCTTTCCCCGCGCCGGCCGCTTCCCGCGGGGCCTCAGCCCAC CCGCGCGTCCCCACGGCCGCCGCGAAGACGTGGGCCCCGGCCTGGGCCTGGCGCCCTCGGCCACCCTCGT GTCGCTGTCGTCCGTGTCCGACTGCAACTCCACGCGTTCACTGCTGCGCTCTGACAGTGACGAGGCCGCA CCGGCCGCGCCCTCCCCACCACCCTCCCCGCCCGCGCCCACACCCACGCCCTCGCCCAGCACCAACCCCC TGGTGGACCTGGAGCTGGAGAGCTTCAAGAAGGACCCCCGCCAGTCGCTCACGCCCACCCACGTCACGGC TGCATGCGCTGTGAGCCGCGGGCACCGGCGGACGCCATCGGACGGGGCGCTGGGGCAGCGGGGGCCGCCC GAGCCCGCGGGCCATGGCCCTGGCCCTCGTGACCTTCTGGACTTCCCCCGCCTGCCCGACCCCCAGGCCC TGTTCCCAGCCCGCCGCCGGCCCCCTGAGTTCCCAGGCCGCCCCACCACCCTGACCTTTGCCCCGAGACC TCGGCCGGCTGCCAGTCGCCCCCGCTTGGACCCCTGGAAACTGGTCTCCTTCGGCCGGACACTCACCATC TCGCCTCCCAGCAGGCCAGACACTCCGGAGAGCCCTGGGCCCCCCAGCGTGCAGCCCACACTGCTGGACA TGGACATGGAGGGGCAGAACCAAGACAGCACAGTGCCCCTGTGCGGGGCCCACGGCTCCCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC218669 representing NM_002446
Red=Cloning site Green=Tags(s) MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGV FPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAE QVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYL HNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLF SKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDF GSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLR RREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASP TLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWG PSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAEDGGSSVPPSPY STPSYLSVPLPAEPSPGARAPWEPTPSAPPARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGE EQRRWLDGLFFPRAGRFPRGLSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAA PAAPSPPPSPPAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPP EPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLTI SPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
AscI-RsrII
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_002446 |
ORF Size | 2862 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002446.4 |
RefSeq Size | 3453 bp |
RefSeq ORF | 2865 bp |
Locus ID | 4294 |
UniProt ID | Q02779 |
Protein Families | Druggable Genome, Protein Kinase |
MW | 104.1 kDa |
Gene Summary | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218669L1 | Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 12,880.00 |
|
RC218669L2 | Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 9,030.00 |
|
RC218669L3 | Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 9,030.00 |
|
RC218669L4 | Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 12,880.00 |
|
RG218669 | MAP3K10 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 7,890.00 |
|
SC118630 | MAP3K10 (untagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 10,488.00 |
|
SC323447 | MAP3K10 (untagged)-Kinase deficient mutant (K125M) of Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 10,488.00 |
其它MAP3K10产品