PNR (TAAR5) (NM_003967) Human Tagged ORF Clone

CAT#: RC218295

TAAR5 (Myc-DDK-tagged)-Human trace amine associated receptor 5 (TAAR5)

ORF Plasmid: DDK tGFP



  "NM_003967" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TAAR5 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PNR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218295 representing NM_003967
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGCTGTCTTCATCCAAGGTGCTGAAGAGCACCCTGCGGCATTCTGCTACCAGGTGAATGGGTCTT
GCCCCAGGACAGTACATACTCTGGGCATCCAGTTGGTCATCTACCTGGCCTGTGCAGCAGGCATGCTGAT
TATCGTGCTAGGGAATGTATTTGTGGCATTTGCTGTGTCCTACTTCAAAGCGCTTCACACGCCCACCAAC
TTCCTGCTGCTCTCCCTGGCCCTGGCTGACATGTTTCTGGGTCTGCTGGTGCTGCCCCTCAGCACCATTC
GCTCAGTGGAGAGCTGCTGGTTCTTCGGGGACTTCCTCTGCCGCCTGCACACCTACCTGGACACCCTCTT
CTGCCTCACCTCCATCTTCCATCTCTGTTTCATTTCCATTGACCGCCACTGTGCCATCTGTGACCCCCTG
CTCTATCCCTCCAAGTTCACAGTGAGGGTGGCTCTCAGGTACATCCTGGCAGGATGGGGGGTGCCCGCAG
CATACACTTCGTTATTCCTCTACACAGATGTGGTAGAGACAAGGCTCAGCCAGTGGCTGGAAGAGATGCC
TTGTGTGGGCAGTTGCCAGCTGCTGCTCAATAAATTTTGGGGCTGGTTAAACTTCCCTTTGTTCTTTGTC
CCCTGCCTCATTATGATCAGCTTGTATGTGAAGATCTTTGTGGTTGCTACCAGACAGGCTCAGCAGATTA
CCACATTGAGCAAAAGCCTGGCTGGGGCTGCCAAGCATGAGAGAAAAGCTGCCAAGACCCTGGGCATTGC
TGTGGGCATATACCTCTTGTGCTGGCTGCCCTTCACCATAGACACGATGGTCGACAGCCTCCTTCACTTT
ATCACACCCCCACTGGTCTTTGACATCTTTATCTGGTTTGCTTACTTCAACTCAGCCTGCAACCCCATCA
TCTATGTCTTTTCCTACCAGTGGTTTCGGAAGGCACTGAAACTCACACTGAGCCAGAAGGTCTTCTCACC
GCAGACACGCACTGTTGATTTGTACCAAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218295 representing NM_003967
Red=Cloning site Green=Tags(s)

MRAVFIQGAEEHPAAFCYQVNGSCPRTVHTLGIQLVIYLACAAGMLIIVLGNVFVAFAVSYFKALHTPTN
FLLLSLALADMFLGLLVLPLSTIRSVESCWFFGDFLCRLHTYLDTLFCLTSIFHLCFISIDRHCAICDPL
LYPSKFTVRVALRYILAGWGVPAAYTSLFLYTDVVETRLSQWLEEMPCVGSCQLLLNKFWGWLNFPLFFV
PCLIMISLYVKIFVVATRQAQQITTLSKSLAGAAKHERKAAKTLGIAVGIYLLCWLPFTIDTMVDSLLHF
ITPPLVFDIFIWFAYFNSACNPIIYVFSYQWFRKALKLTLSQKVFSPQTRTVDLYQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003967
ORF Size 1011 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003967.2
RefSeq Size 1014 bp
RefSeq ORF 1014 bp
Locus ID 9038
UniProt ID O14804
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 38.1 kDa
Gene Summary Olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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