SNX26 (ARHGAP33) (NM_052948) Human Tagged ORF Clone

CAT#: RC218143

ARHGAP33 (Myc-DDK-tagged)-Human Rho GTPase activating protein 33 (ARHGAP33), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_052948" in other vectors (4)

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CNY 7,840.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Polyclonal Antibody against SNX26
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms NOMA-GAP; SNX26; TCGAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218143 representing NM_052948
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCACGCAGCACTGACAGCCTGGATGGCCCAGGGGAGGGCTCGGTGCAGCCTCTACCCACTGCTG
GGGGGCCCAGTGTGAAGGGGAAGCCTGGGAAGAGGCTCTCAGCTCCTCGAGGCCCCTTCCCGCGGCTGGC
TGACTGCGCCCATTTCCACTACGAGAACGTTGACTTTGGCCACATTCAGCTCCTGCTGTCTCCAGACCGT
GAAGGGCCCAGCCTCTCTGGAGAGAATGAGCTGGTGTTCGGGGTGCAGGTGACCTGTCAGGGCCGTTCCT
GGCCGGTTCTCCGGAGTTACGATGACTTTCGTTCCCTGGATGCCCACCTCCACCGGTGCATATTTGACCG
GAGGTTCTCCTGCCTTCCGGAGCTTCCCCCGCCCCCCGAGGGTGCCAGGGCTGCCCAGATGCTGGTGCCA
CTGCTGCTGCAGTACCTGGAGACACTGTCAGGACTGGTGGACAGTAACCTCAACTGCGGGCCTGTGCTCA
CCTGGATGGAGCTGGACAATCACGGCCGGCGACTGCTCCTCAGTGAGGAGGCGTCACTCAATATCCCTGC
AGTGGCGGCCGCCCATGTGATCAAACGGTATACAGCCCAGGCGCCAGATGAGCTGTCCTTTGAGGTGGGA
GACATTGTCTCGGTGATCGACATGCCACCCACAGAGGATCGGAGCTGGTGGCGGGGCAAGCGAGGCTTCC
AGGTCGGGTTCTTCCCCAGTGAGTGTGTGGAACTCTTCACAGAGCGGCCAGGTCCGGGCCTGAAGGCGGA
TGCCGATGGCCCCCCATGTGGCATCCCGGCTCCCCAGGGTATCTCGTCTCTGACCTCAGCTGTGCCACGG
CCTCGTGGGAAGCTGGCCGGCCTGCTCCGCACCTTCATGCGCTCCCGCCCTTCTCGGCAGCGGCTGCGGC
AGCGGGGAATCCTGCGACAGAGGGTGTTTGGCTGCGATCTTGGCGAGCACCTCAGCAACTCAGGCCAGGA
TGTGCCCCAGGTGCTGCGCTGCTGCTCCGAGTTCATTGAGGCCCACGGGGTGGTGGATGGGATCTACCGG
CTCTCAGGCGTGTCTTCCAACATCCAGAGGCTTCGGCACGAGTTTGACAGTGAGAGGATCCCGGAGCTGT
CTGGCCCTGCATTCCTGCAGGACATCCACAGCGTGTCCTCCCTCTGCAAGCTCTACTTCCGAGAGCTTCC
GAACCCTCTGCTCACCTACCAGCTCTATGGGAAGTTCAGTGAGGCCATGTCAGTGCCTGGGGAGGAGGAG
CGTCTGGTGCGGGTGCACGATGTCATCCAGCAGCTGCCCCCACCACATTACAGGACCCTGGAGTACCTGC
TGAGGCACCTGGCCCGCATGGCGAGACACAGTGCCAACACCAGCATGCATGCCCGCAACCTGGCCATTGT
CTGGGCACCCAACCTGCTACGGTCCATGGAGCTGGAGTCAGTGGGAATGGGTGGCGCGGCGGCGTTCCGG
GAAGTTCGGGTGCAGTCGGTGGTGGTGGAGTTTCTGCTCACCCATGTGGACGTCCTGTTCAGCGACACCT
TCACCTCCGCCGGCCTCGACCCTGCAGGCCGCTGCCTGCTCCCCAGGCCCAAGTCCCTTGCGGGCAGCTG
CCCCTCCACCCGCCTGCTGACGCTGGAGGAAGCCCAGGCACGCACCCAGGGCCGGCTGGGGACGCCCACG
GAGCCCACAACTCCCAAGGCCCCGGCCTCACCTGCGGAAAGGAGGAAAGGGGAGAGAGGGGAGAAGCAGC
GGAAGCCAGGGGGCAGCAGCTGGAAGACGTTCTTTGCACTGGGCCGGGGCCCCAGTGTCCCTCGAAAGAA
GCCCCTGCCCTGGCTGGGGGGCACCCGTGCCCCACCGCAGCCTTCAGGCAGCAGACCCGACACCGTCACA
CTGAGATCTGCCAAGAGCGAGGAGTCTCTGTCATCGCAGGCCAGCGGGGCTGAACTGCTGGGGGCTGGGG
GAGCACCTGCCTCAGCCACCCCAACACCAGCTCTCAGCCCCGGCCGGAGCCTGCGCCCCCATCTCATACC
CCTGCTGCTGCGAGGAGCCGAGGCCCCGCTGACTGACGCCTGCCAGCAGGAGATGTGCAGCAAGCTCCGG
GGAGCCCAGGGCCCACTCGGTCCTGATATGGAGTCACCACTGCCACCCCCTCCCCTGTCTCTCCTGCGCC
CTGGGGGTGCCCCACCCCCGCCCCCTAAGAACCCAGCACGCCTCATGGCCCTGGCCCTGGCTGAGCGGGC
TCAGCAGGTGGCCGAGCAACAGAGCCAGCAGGAGTGTGGGGGCACCCCACCTGCTTCCCAATCCCCCTTC
CACCGCTCGCTGTCTCTGGAGGTGGGCGGGGAGCCCCTGGGGACCTCAGGGAGTGGGCCACCTCCCAACT
CCCTAGCACACCCGGGTGCCTGGGTCCCGGGACCCCCACCCTACTTACCAAGGCAACAAAGTGATGGGAG
CCTGCTGAGGAGCCAGCGGCCCATGGGGACCTCAAGGAGGGGACTCCGAGGCCCTGCCCAGGTCAGTGCC
CAGCTCAGGGCAGGTGGCGGGGGCAGGGATGCGCCAGAGGCAGCAGCCCAGTCCCCATGTTCTGTCCCCT
CACAGGTTCCTACCCCCGGCTTCTTCTCCCCAGCCCCCAGGGAGTGCCTGCCACCCTTCCTCGGGGTCCC
CAAGCCAGGCTTGTACCCCCTGGGCCCCCCATCCTTCCAGCCCAGTTCCCCAGCCCCAGTCTGGAGGAGC
TCTCTGGGCCCCCCTGCACCACTCGACAGGGGAGAGAACCTGTACTATGAGATCGGGGCAAGTGAGGGGT
CCCCCTATTCTGGCCCCACCCGCTCCTGGAGTCCCTTTCGCTCCATGCCCCCCGACAGGCTCAATGCCTC
CTACGGCATGCTTGGCCAATCACCCCCACTCCACAGGTCCCCCGACTTCCTGCTCAGCTACCCGCCAGCC
CCCTCCTGCTTTCCCCCTGACCACCTTGGCTACTCAGCCCCCCAGCACCCTGCTCGGCGCCCTACACCGC
CTGAGCCCCTCTACGTCAACCTAGCTCTAGGGCCCAGGGGTCCCTCACCTGCCTCTTCCTCCTCCTCTTC
CCCTCCTGCCCACCCCCGAAGCCGTTCAGATCCCGGTCCCCCAGTCCCCCGCCTTCCCCAGAAACAACGG
GCACCCTGGGGACCCCGTACCCCTCATAGGGTGCCGGGTCCCTGGGGCCCTCCTGAGCCTCTCCTGCTCT
ACAGGGCAGCCCCGCCAGCCTACGGAAGGGGGGGCGAGCTCCACCGAGGGTCCTTGTACAGAAATGGAGG
GCAAAGAGGGGAGGGGGCTGGTCCCCCACCCCCTTACCCCACTCCCAGCTGGTCCCTCCACTCTGAGGGC
CAGACCCGAAGCTACTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218143 representing NM_052948
Red=Cloning site Green=Tags(s)

MVARSTDSLDGPGEGSVQPLPTAGGPSVKGKPGKRLSAPRGPFPRLADCAHFHYENVDFGHIQLLLSPDR
EGPSLSGENELVFGVQVTCQGRSWPVLRSYDDFRSLDAHLHRCIFDRRFSCLPELPPPPEGARAAQMLVP
LLLQYLETLSGLVDSNLNCGPVLTWMELDNHGRRLLLSEEASLNIPAVAAAHVIKRYTAQAPDELSFEVG
DIVSVIDMPPTEDRSWWRGKRGFQVGFFPSECVELFTERPGPGLKADADGPPCGIPAPQGISSLTSAVPR
PRGKLAGLLRTFMRSRPSRQRLRQRGILRQRVFGCDLGEHLSNSGQDVPQVLRCCSEFIEAHGVVDGIYR
LSGVSSNIQRLRHEFDSERIPELSGPAFLQDIHSVSSLCKLYFRELPNPLLTYQLYGKFSEAMSVPGEEE
RLVRVHDVIQQLPPPHYRTLEYLLRHLARMARHSANTSMHARNLAIVWAPNLLRSMELESVGMGGAAAFR
EVRVQSVVVEFLLTHVDVLFSDTFTSAGLDPAGRCLLPRPKSLAGSCPSTRLLTLEEAQARTQGRLGTPT
EPTTPKAPASPAERRKGERGEKQRKPGGSSWKTFFALGRGPSVPRKKPLPWLGGTRAPPQPSGSRPDTVT
LRSAKSEESLSSQASGAELLGAGGAPASATPTPALSPGRSLRPHLIPLLLRGAEAPLTDACQQEMCSKLR
GAQGPLGPDMESPLPPPPLSLLRPGGAPPPPPKNPARLMALALAERAQQVAEQQSQQECGGTPPASQSPF
HRSLSLEVGGEPLGTSGSGPPPNSLAHPGAWVPGPPPYLPRQQSDGSLLRSQRPMGTSRRGLRGPAQVSA
QLRAGGGGRDAPEAAAQSPCSVPSQVPTPGFFSPAPRECLPPFLGVPKPGLYPLGPPSFQPSSPAPVWRS
SLGPPAPLDRGENLYYEIGASEGSPYSGPTRSWSPFRSMPPDRLNASYGMLGQSPPLHRSPDFLLSYPPA
PSCFPPDHLGYSAPQHPARRPTPPEPLYVNLALGPRGPSPASSSSSSPPAHPRSRSDPGPPVPRLPQKQR
APWGPRTPHRVPGPWGPPEPLLLYRAAPPAYGRGGELHRGSLYRNGGQRGEGAGPPPPYPTPSWSLHSEG
QTRSYC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_052948
ORF Size 3378 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052948.4
RefSeq Size 3859 bp
RefSeq ORF 3381 bp
Locus ID 115703
UniProt ID O14559
MW 120.9 kDa
Gene Summary This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Alternative splice variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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