TXNDC5 (NM_030810) Human Tagged ORF Clone

CAT＃: RC218032

TrueORF^®

TXNDC5 (Myc-DDK-tagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_030810" in other vectors (7)

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CNY 3,990.00

货期*

2周

规格

Cited in 1 publication.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA507281

TXNDC5 mouse monoclonal antibody, clone OTI2E6 (formerly 2E6)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ENDOPDI; ERP46; HCC-2; HCC2; PDIA15; STRF8; UNQ364
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC218032 representing NM_030810 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCGCGCCCAGGACGCCTCCTCCCGCTGCTGGCCCGGCCGGCGGCCCTGACTGCGCTGCTGCTGC TGCTGCTGGGCCATGGCGGCGGCGGGCGCTGGGGCGCCCGGGCCCAGGAGGCGGCGGCGGCGGCGGCGGA CGGGCCCCCCGCGGCAGACGGCGAGGACGGACAGGACCCGCACAGCAAGCACCTGTACACGGCCGACATG TTCACGCACGGGATCCAGAGCGCCGCGCACTTCGTCATGTTCTTCGCGCCCTGGTGTGGACACTGCCAGC GGCTGCAGCCGACTTGGAATGACCTGGGAGACAAATACAACAGCATGGAAGATGCCAAAGTCTATGTGGC TAAAGTGGACTGCACGGCCCACTCCGACGTGTGCTCCGCCCAGGGGGTGCGAGGATACCCCACCTTAAAG CTTTTCAAGCCAGGCCAAGAAGCTGTGAAGTACCAGGGTCCTCGGGACTTCCAGACACTGGAAAACTGGA TGCTGCAGACACTGAACGAGGAGCCAGTGACACCAGAGCCGGAAGTGGAACCGCCCAGTGCCCCCGAGCT CAAGCAAGGGCTGTATGAGCTCTCAGCAAGCAACTTTGAGCTGCACGTTGCACAAGGCGACCACTTTATC AAGTTCTTCGCTCCGTGGTGTGGTCACTGCAAAGCCCTGGCTCCAACCTGGGAGCAGCTGGCTCTGGGCC TTGAACATTCCGAAACTGTCAAGATTGGCAAGGTTGATTGTACACAGCACTATGAACTCTGCTCCGGAAA CCAGGTTCGTGGCTATCCCACTCTTCTCTGGTTCCGAGATGGGAAAAAGGTGGATCAGTACAAGGGAAAG CGGGATTTGGAGTCACTGAGGGAGTACGTGGAGTCGCAGCTGCAGCGCACAGAGACTGGAGCGACGGAGA CCGTCACGCCCTCAGAGGCCCCGGTGCTGGCAGCTGAGCCCGAGGCTGACAAGGGCACTGTGTTGGCACT CACTGAAAATAACTTCGATGACACCATTGCAGAAGGAATAACCTTCATCAAGTTTTATGCTCCATGGTGT GGTCATTGTAAGACTCTGGCTCCTACTTGGGAGGAACTCTCTAAAAAGGAATTCCCTGGTCTGGCGGGGG TCAAGATCGCCGAAGTAGACTGCACTGCTGAACGGAATATCTGCAGCAAGTATTCGGTACGAGGCTACCC CACGTTATTGCTTTTCCGAGGAGGGAAGAAAGTCAGTGAGCACAGTGGAGGCAGAGACCTTGACTCGTTA CACCGCTTTGTCCTGAGCCAAGCGAAAGACGAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC218032 representing NM_030810 Red=Cloning site Green=Tags(s) MPARPGRLLPLLARPAALTALLLLLLGHGGGGRWGARAQEAAAAAADGPPAADGEDGQDPHSKHLYTADM FTHGIQSAAHFVMFFAPWCGHCQRLQPTWNDLGDKYNSMEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLK LFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFI KFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGK RDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWC GHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSL HRFVLSQAKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_030810
ORF Size	1296 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030810.5
RefSeq Size	2970 bp
RefSeq ORF	1299 bp
Locus ID	81567
UniProt ID	Q8NBS9
Domains	thiored
Protein Families	Druggable Genome
MW	44.4 kDa
Gene Summary	This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal endoplasmic reticulum (ER)-signal sequence, three catalytically active thioredoxin domains and a C-terminal ER-retention sequence. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S5 gene. [provided by RefSeq, Dec 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Endoplasmic reticulum protein ERp46 in prostate adenocarcinoma ,Duivenvoorden, WCM;Hopmans, SN;Austin, RC;Pinthus, JH;, Oncol Lett ,PubMed ID 28521463 [TXNDC5]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC218032L1	Lenti-ORF clone of TXNDC5 (Myc-DDK-tagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC218032L2	Lenti-ORF clone of TXNDC5 (mGFP-tagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC218032L3	Lenti-ORF clone of TXNDC5 (Myc-DDK-tagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC218032L4	Lenti-ORF clone of TXNDC5 (mGFP-tagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG218032	TXNDC5 (tGFP-tagged) - Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00
SC109657	TXNDC5 (untagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,496.00
SC317697	TXNDC5 (untagged)-Human thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 6,940.00