RbAp46 (RBBP7) (NM_002893) Human Tagged ORF Clone

CAT#: RC217969

RBBP7 (Myc-DDK-tagged)-Human retinoblastoma binding protein 7 (RBBP7), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_002893" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


RBBP7 (RbAp46) mouse monoclonal antibody, clone OTI5A4 (formerly 5A4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RbAp46
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217969 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGTAAAGAGATGTTTGAAGATACTGTGGAGGAGCGTGTCATCAATGAAGAATATAAAATCTGGA
AGAAGAATACACCGTTTCTATATGACCTGGTTATGACCCATGCTCTTCAGTGGCCCAGTCTTACCGTTCA
GTGGCTTCCTGAAGTGACTAAACCTGAAGGAAAAGATTATGCCCTTCATTGGCTAGTGCTGGGGACTCAT
ACGTCTGATGAGCAGAATCATCTGGTGGTTGCTCGAGTACATATTCCCAATGATGATGCACAGTTTGATG
CTTCCCATTGTGACAGTGACAAGGGTGAATTTGGTGGCTTTGGTTCTGTAACAGGAAAAATTGAATGTGA
AATTAAAATCAATCACGAAGGAGAAGTAAACCGTGCTCGTTACATGCCGCAGAATCCTCACATCATTGCT
ACAAAAACACCATCTTCTGATGTGTTGGTTTTTGACTATACAAAACACCCTGCTAAACCAGACCCAAGTG
GAGAATGTAATCCTGATCTCAGATTAAGAGGTCACCAGAAGGAAGGCTATGGTCTCTCCTGGAATTCAAA
TTTGAGTGGACATCTCCTAAGTGCATCTGATGACCATACTGTTTGTCTGTGGGATATAAACGCAGGACCA
AAAGAAGGCAAAATTGTGGATGCTAAAGCCATCTTTACTGGCCACTCAGCTGTTGTAGAGGATGTGGCCT
GGCACCTGCTGCACGAGTCATTGTTTGGATCTGTTGCTGATGATCAGAAACTTATGATATGGGACACCAG
GTCCAATACCACCTCCAAGCCGAGTCACTTGGTGGATGCGCACACTGCCGAAGTCAACTGCCTCTCATTC
AATCCCTACAGCGAATTTATTCTAGCCACCGGCTCTGCGGATAAGACCGTAGCTTTATGGGATCTGCGTA
ACTTAAAATTAAAACTCCATACCTTCGAATCTCATAAAGATGAAATTTTCCAGGTCCACTGGTCTCCACA
TAATGAAACTATTCTGGCTTCAAGTGGTACTGACCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGG
GAAGAACAATCAGCAGAAGATGCAGAAGATGGGCCTCCAGAACTCCTGTTTATTCATGGAGGACACACTG
CTAAGATTTCAGATTTTAGCTGGAACCCCAATGAGCCTTGGGTCATTTGCTCAGTGTCTGAGGATAACAT
CATGCAGATATGGCAAATGGCTGAAAATATTTACAATGATGAAGAGTCAGATGTCACGACATCCGAACTG
GAGGGACAAGGATCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217969 protein sequence
Red=Cloning site Green=Tags(s)

MASKEMFEDTVEERVINEEYKIWKKNTPFLYDLVMTHALQWPSLTVQWLPEVTKPEGKDYALHWLVLGTH
TSDEQNHLVVARVHIPNDDAQFDASHCDSDKGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIA
TKTPSSDVLVFDYTKHPAKPDPSGECNPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHTVCLWDINAGP
KEGKIVDAKAIFTGHSAVVEDVAWHLLHESLFGSVADDQKLMIWDTRSNTTSKPSHLVDAHTAEVNCLSF
NPYSEFILATGSADKTVALWDLRNLKLKLHTFESHKDEIFQVHWSPHNETILASSGTDRRLNVWDLSKIG
EEQSAEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQIWQMAENIYNDEESDVTTSEL
EGQGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002893
ORF Size 1275 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002893.4
RefSeq Size 2021 bp
RefSeq ORF 1278 bp
Locus ID 5931
UniProt ID Q16576
Domains WD40
Protein Families Druggable Genome
MW 47.8 kDa
Gene Summary This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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