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DEF6 (NM_022047) Human Tagged ORF Clone
CAT＃: RC217877
DEF6 (Myc-DDK-tagged)-Human differentially expressed in FDCP 6 homolog (mouse) (DEF6)
ORF Plasmid: tGFP
"NM_022047" in other vectors (6)
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CNY 4,696.00
CNY 7,410.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IBP; SLAT; SWAP70L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217877 representing NM_022047
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGCGCAAGGAACTGCTCAAGTCCATCTGGTACGCCTTTACCGCGCTGGACGTGGAGAAGAGTG GCAAAGTCTCCAAGTCCCAGCTCAAGGTGCTGTCCCACAACCTGTACACGGTCCTGCACATCCCCCATGA CCCCGTGGCCCTGGAGGAACACTTCCGAGATGATGATGACGGCCCTGTGTCCAGCCAGGGATACATGCCC TACCTCAACAAGTACATCCTGGACAAGGTGGAGGAGGGGGCTTTTGTTAAAGAGCACTTTGATGAGCTGT GCTGGACGCTGACGGCCAAGAAGAACTATCGGGCAGATAGCAACGGGAACAGTATGCTCTCCAATCAGGA TGCCTTCCGCCTCTGGTGCCTCTTCAACTTCCTGTCTGAGGACAAGTACCCTCTGATCATGGTTCCTGAT GAGGTGGAATACCTGCTGAAAAAGGTACTCAGCAGCATGAGCTTGGAGGTGAGCTTGGGTGAGCTGGAGG AGCTTCTGGCCCAGGAGGCCCAGGTGGCCCAGACCACCGGGGGGCTCAGCGTCTGGCAGTTCCTGGAGCT CTTCAATTCGGGCCGCTGCCTGCGGGGCGTGGGCCGGGACACCCTCAGCATGGCCATCCACGAGGTCTAC CAGGAGCTCATCCAAGATGTCCTGAAGCAGGGCTACCTGTGGAAGCGAGGGCACCTGAGAAGGAACTGGG CCGAACGCTGGTTCCAGCTGCAGCCCAGCTGCCTCTGCTACTTTGGGAGTGAAGAGTGCAAAGAGAAAAG GGGCATTATCCCGCTGGATGCACACTGCTGCGTGGAGGTGCTGCCAGACCGCGACGGAAAGCGCTGCATG TTCTGTGTGAAGACAGCCACCCGCACGTATGAGATGAGCGCCTCAGACACGCGCCAGCGCCAGGAGTGGA CAGCTGCCATCCAGATGGCGATCCGGCTGCAGGCCGAGGGGAAGACGTCCCTACACAAGGACCTGAAGCA GAAACGGCGCGAGCAGCGGGAGCAGCGGGAGCGGCGCCGGGCGGCCAAGGAAGAGGAGCTGCTGCGGCTG CAGCAGCTGCAGGAGGAGAAGGAGCGGAAGCTGCAGGAGCTGGAGCTGCTGCAGGAGGCGCAGCGGCAGG CCGAGCGGCTGCTGCAGGAGGAGGAGGAACGGCGCCGCAGCCAGCACCGCGAGCTGCAGCAGGCGCTCGA GGGCCAACTGCGCGAGGCGGAGCAGGCCCGGGCCTCCATGCAGGCTGAGATGGAGCTGAAGGAGGAGGAG GCTGCCCGGCAGCGGCAGCGCATCAAGGAGCTGGAGGAGATGCAGCAGCGGTTGCAGGAGGCCCTGCAAC TAGAGGTGAAAGCTCGGCGAGATGAAGAATCTGTGCGAATCGCTCAGACCAGACTGCTGGAAGAGGAGGA AGAGAAGCTGAAGCAGTTGATGCAGCTGAAGGAGGAGCAGGAGCGCTACATCGAACGGGCGCAGCAGGAG AAGGAAGAGCTGCAGCAGGAGATGGCACAGCAGAGCCGCTCCCTGCAGCAGGCCCAGCAGCAGCTGGAGG AGGTGCGGCAGAACCGGCAGAGGGCTGACGAGGATGTGGAGGCTGCCCAGAGAAAACTGCGCCAGGCCAG CACCAACGTGAAACACTGGAATGTCCAGATGAACCGGCTGATGCATCCAATTGAGCCTGGAGATAAGCGT CCGGTCACCAGCAGCTCCTTCTCAGGCTTCCAGCCCCCTCTGCTTGCCCACCGTGACTCCTCCCTAAAGC GCCTGACCCGCTGGGGATCCCAGGGCAACAGGACCCCCTCGCCCAACAGCAATGAGCAGCAGAAGTCCCT CAATGGTGGGGATGAGGCTCCTGCCCCGGCTTCCACCCCTCAGGAAGATAAACTGGATCCAGCACCAGAA AAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217877 representing NM_022047
Red=Cloning site Green=Tags(s) MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLYTVLHIPHDPVALEEHFRDDDDGPVSSQGYMP YLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRADSNGNSMLSNQDAFRLWCLFNFLSEDKYPLIMVPD EVEYLLKKVLSSMSLEVSLGELEELLAQEAQVAQTTGGLSVWQFLELFNSGRCLRGVGRDTLSMAIHEVY QELIQDVLKQGYLWKRGHLRRNWAERWFQLQPSCLCYFGSEECKEKRGIIPLDAHCCVEVLPDRDGKRCM FCVKTATRTYEMSASDTRQRQEWTAAIQMAIRLQAEGKTSLHKDLKQKRREQREQRERRRAAKEEELLRL QQLQEEKERKLQELELLQEAQRQAERLLQEEEERRRSQHRELQQALEGQLREAEQARASMQAEMELKEEE AARQRQRIKELEEMQQRLQEALQLEVKARRDEESVRIAQTRLLEEEEEKLKQLMQLKEEQERYIERAQQE KEELQQEMAQQSRSLQQAQQQLEEVRQNRQRADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIEPGDKR PVTSSSFSGFQPPLLAHRDSSLKRLTRWGSQGNRTPSPNSNEQQKSLNGGDEAPAPASTPQEDKLDPAPE N TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022047 |
| ORF Size | 1893 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022047.2, NP_071330.2 |
| RefSeq Size | 2320 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 50619 |
| UniProt ID | Q9H4E7 |
| Domains | PH |
| Protein Families | Druggable Genome |
| MW | 73.7 kDa |
| Gene Summary | DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217877L1 | Lenti ORF clone of Human differentially expressed in FDCP 6 homolog (mouse) (DEF6), Myc-DDK-tagged |
CNY 7,096.00 |
|
| RC217877L2 | Lenti ORF clone of Human differentially expressed in FDCP 6 homolog (mouse) (DEF6), mGFP tagged |
CNY 5,890.00 |
|
| RC217877L3 | Lenti ORF clone of Human differentially expressed in FDCP 6 homolog (mouse) (DEF6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217877L4 | Lenti ORF clone of Human differentially expressed in FDCP 6 homolog (mouse) (DEF6), mGFP tagged |
CNY 5,890.00 |
|
| RG217877 | DEF6 (tGFP-tagged) - Human differentially expressed in FDCP 6 homolog (mouse) (DEF6) |
CNY 6,296.00 |
|
| SC111258 | DEF6 (untagged)-Human differentially expressed in FDCP 6 homolog (mouse) (DEF6) |
CNY 10,170.00 |
