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TRIM58 (NM_015431) Human Tagged ORF Clone
CAT＃: RC217706
TRIM58 (Myc-DDK-tagged)-Human tripartite motif containing 58 (TRIM58)
ORF Plasmid: tGFP
"NM_015431" in other vectors (6)
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CNY 3,656.00
CNY 4,280.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BIA2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217706 representing NM_015431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGCGCCGCCCGGGGAGCGGCTGCGCGAGGATGCGCGGTGCCCGGTGTGCCTGGATTTCCTGC AGGAGCCGGTCAGCGTGGACTGCGGCCACAGCTTCTGCCTCAGGTGCATCTCCGAGTTCTGCGAGAAGTC GGACGGCGCGCAGGGCGGCGTCTACGCCTGTCCGCAGTGCCGGGGCCCCTTCCGGCCCTCGGGCTTTCGC CCCAACCGGCAGCTGGCGGGCCTGGTGGAGAGCGTGCGGCGGCTGGGGTTGGGCGCGGGGCCCGGGGCGC GGCGATGCGCGCGGCACGGCGAGGACCTGAGCCGCTTCTGCGAGGAGGACGAGGCGGCGCTGTGCTGGGT GTGCGACGCCGGCCCCGAGCACAGGACGCACCGCACGGCGCCGCTGCAGGAGGCCGCCGGCAGCTACCAG GTAAAGCTCCAGATGGCTCTGGAACTTATGAGGAAAGAGTTGGAGGACGCCTTGACTCAGGAGGCCAACG TGGGGAAAAAGACTGTCATTTGGAAGGAGAAAGTGGAAATGCAGAGGCAGCGCTTCAGATTGGAGTTTGA GAAGCATCGTGGCTTTCTGGCCCAGGAGGAGCAACGGCAGCTGAGGCGGCTGGAGGCGGAGGAGCGAGCG ACGCTGCAGAGACTGCGGGAGAGCAAGAGCCGGCTGGTCCAGCAGAGCAAGGCCCTGAAGGAGCTGGCGG ATGAGCTGCAGGAGAGGTGCCAGCGCCCGGCCCTGGGTCTGCTGGAGGGTGTGAGAGGAGTCCTGAGCAG AAGTAAGGCTGTCACAAGGCTGGAAGCAGAGAACATCCCCATGGAACTGAAGACAGCATGCTGCATCCCT GGGAGGAGGGAGCTCTTAAGGAAGTTCCAAGTGGATGTAAAGCTGGATCCCGCCACGGCGCACCCGAGTC TGCTCTTGACCGCCGACCTGCGCAGTGTGCAGGATGGAGAACCATGGAGGGATGTCCCCAACAACCCTGA GCGATTTGACACATGGCCCTGCATCCTGGGTTTGCAGAGCTTCTCATCAGGGAGGCATTACTGGGAGGTT CTGGTGGGAGAAGGAGCAGAGTGGGGTTTAGGGGTCTGTCAAGACACACTGCCAAGAAAGGGGGAAACCA CGCCATCTCCTGAGAATGGGGTCTGGGCCCTGTGGCTGCTGAAAGGGAATGAGTACATGGTCCTTGCCTC CCCATCAGTGCCTCTTCTCCAACTGGAAAGTCCTCGCTGCATTGGGATTTTCTTGGACTATGAAGCCGGT GAAATTTCATTCTACAATGTCACAGATGGATCTTATATCTACACATTCAACCAACTCTTCTCTGGTCTTC TTCGGCCTTACTTTTTCATCTGTGATGCAACTCCTCTTATCTTGCCACCCACAACAATAGCAGGGTCAGG AAATTGGGCATCCAGGGATCATTTAGATCCTGCTTCTGATGTAAGAGATGATCATCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217706 representing NM_015431
Red=Cloning site Green=Tags(s) MASAPPGERLREDARCPVCLDFLQEPVSVDCGHSFCLRCISEFCEKSDGAQGGVYACPQCRGPFRPSGFR PNRQLAGLVESVRRLGLGAGPGARRCARHGEDLSRFCEEDEAALCWVCDAGPEHRTHRTAPLQEAAGSYQ VKLQMALELMRKELEDALTQEANVGKKTVIWKEKVEMQRQRFRLEFEKHRGFLAQEEQRQLRRLEAEERA TLQRLRESKSRLVQQSKALKELADELQERCQRPALGLLEGVRGVLSRSKAVTRLEAENIPMELKTACCIP GRRELLRKFQVDVKLDPATAHPSLLLTADLRSVQDGEPWRDVPNNPERFDTWPCILGLQSFSSGRHYWEV LVGEGAEWGLGVCQDTLPRKGETTPSPENGVWALWLLKGNEYMVLASPSVPLLQLESPRCIGIFLDYEAG EISFYNVTDGSYIYTFNQLFSGLLRPYFFICDATPLILPPTTIAGSGNWASRDHLDPASDVRDDHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015431 |
| ORF Size | 1458 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015431.2, NP_056246.2 |
| RefSeq Size | 2594 bp |
| RefSeq ORF | 1461 bp |
| Locus ID | 25893 |
| UniProt ID | Q8NG06 |
| Domains | zf-B_box, RING, SPRY, PRY |
| Protein Families | Druggable Genome |
| MW | 54.6 kDa |
| Gene Summary | E3 ubiquitin ligase induced during late erythropoiesis. Directly binds and ubiquitinates the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TRIM58 Restrains Intestinal Mucosal Inflammation by Negatively Regulating TLR2 in Myeloid Cells
,Eyking, A;Ferber, F;Köhler, S;Reis, H;Cario, E;,
J. Immunol.
,PubMed ID 31383741
[TRIM58]
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Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217706L1 | Lenti ORF clone of Human tripartite motif containing 58 (TRIM58), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC217706L2 | Lenti ORF clone of Human tripartite motif containing 58 (TRIM58), mGFP tagged |
CNY 6,180.00 |
|
| RC217706L3 | Lenti ORF clone of Human tripartite motif containing 58 (TRIM58), Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC217706L4 | Lenti ORF clone of Human tripartite motif containing 58 (TRIM58), mGFP tagged |
CNY 6,180.00 |
|
| RG217706 | TRIM58 (tGFP-tagged) - Human tripartite motif containing 58 (TRIM58) |
CNY 5,256.00 |
|
| SC313679 | TRIM58 (untagged)-Human tripartite motif containing 58 (TRIM58) |
CNY 7,790.00 |
