ZFP106 (ZNF106) (NM_022473) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SH3BP3; ZFP106; ZNF474
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC217612 representing NM_022473 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGGGGAGAATTGAATGTCCCTCATCTCCCTCTTTCCCTAGGGACATTAGTCATGAGTGCCGAG TGTGCGGGGTCACAGAAGTGGGTCTTTCTGCATATGCAAAGCACATTTCTGGCCAGTTGCACAAAGATAA CGTTGATGCCCAGGAAAGAGAAGATGATGGAAAAGGAGAGGAAGAGGAAGAAGATTATTTTGACAAGGAA CTCATTCAGTTAATAAAACAAAGGAAAGAACAAAGTCGACAAGATGAACCTTCCAATAGCAACCAAGAAA TAAACTCTGATGACAGACGACCCCAATGGAGACGAGAAGACCGAATTCCTTACCAAGACAGAGAGAGTTA CAGTCAGCCTGCATGGCATCATCGTGGACCTCCACAGCGGGATTGGAAATGGGAAAAAGATGGCTTTAAT AATACTAGGAAAAACAGCTTTCCACATTCTTTGAGGAATGGTGGTGGACCAAGAGGACGTTCCGGGTGGC ATAAGGGTGTTGCAGGAGGCTCCTCGACTTGGTTTCACAACCATAGTAATTCTGGAGGTGGTTGGCTTTC AAATAGTGGAGCAGTAGATTGGAATCATAATGGTACAGGAAGGAATTCCAGTTGGCTTTCTGAAGGAACA GGTGGCTTTTCCAGTTGGCATATGAACAACAGTAACGGAAACTGGAAATCCAGTGTACGTAGTACAAATA ATTGGAATTACAGTGGCCCTGGAGACAAATTTCAACCAGGCAGAAACAGAAATTCTAACTGTCAAATGGA AGACATGACTATGCTATGGAACAAGAAATCTAATAAGTCAAACAAATACAGTCACGACAGATATAATTGG CAGCGGCAAGAAAATGACAAACTTGGTACAGTTGCCACATATAGAGGTCCTTCTGAAGGATTTACAAGTG ATAAATTTCCTTCAGAAGGCTTACTCGACTTCAATTTTGAGCAGCTGGAAAGCCAAACCACTAAACAAGC AGACACTGCTACTTCCAAAGTTAGTGGAAAGAATGGCAGTGCGGCAAGGGAAAAGCCTCGTCGCTGGACG CCTTACCCTTCTCAGAAGACTCTGGATTTACAGTCGGGATTGAAAGACATCACTGGTAACAAGTCAGAAA TGATAGAGAAACCTCTCTTTGATTTTAGCTTGATAACTACAGGAATACAGGAGCCCCAAACTGATGAAAC ACGTAATTCCCCAACACAGAAAACACAAAAAGAAATACATACTGGATCTCTTAATCACAAGGCCTCTTCT GATTCCGCTGCTTCCTTCGAGGTGGTGAGACAGTGCCCCACTGCAGAAAAACCTGAACAAGAGCATACAC CAAATAAAATGCCATCATTGAAATCCCCACTCCTTCCATGTCCAGCCACTAAATCATTGTCTCAAAAGCA AGATCCAAAGAATATCTCAAAAAACACCAAAACAAATTTTTTTTCCCCTGGAGAACACTCAAATCCCTCG AACAAGCCCACTGTGGAAGATAACCATGGTCCTTACATATCCAAACTGCGTAGTTCATGTCCTCATGTTT TAAAAGGGAATAAAAGTACATTTGGCTCTCAAAAGCAATCTGGTGATAATTTAAATGATACTTTACGAAA GGCCAAAGAGGTGCTACAGTGTCATGAGTCATTGCAAAATCCACTTCTTAGCACTTCTAAAAGTACCAGG AACTATGCAAAAGCAAGTAGAAATGTAGAAGAATCTGAAAAAGGGTCTTTGAAAATTGAGTTTCAAGTGC ACGCACTAGAAGATGAAAGTGATGGAGAGACATCTGACACGGAAAAGCATGGAACAAAAATTGGAACCCT AGGTTCTGCAACTACAGAATTGTTATCTGGCAGCACTCGAACTGCTGATGAGAAAGAGGAGGATGACCGC ATCCTGAAGACTTCTAGAGAGCTATCCACTTCCCCATGTAATCCCATAGTTCGCCAGAAAGAATCTGAAT TACAAATGACATCTGCAGCCAGTCCACACCCTGGCTTATTGCTAGACTTGAAAACCTCTCTAGAAGATGC ACAGGTTGATGACTCTATTAAATCTCATGTATCTTATGAAACAGAAGGCTTTGAGAGTGCTAGCTTGGAT GCAGAGCTTCAAAAAAGTGACATCAGTCAGCCCTCGGGCCCTCTCCTGCCTGAACTAAGTAAGCTTGGCT TTCCTGCCTCACTTCAGAGGGATCTAACCCGGCACATTAGTTTGAAGAGCAAAACTGGAGTACACCTTCC TGAGCCAAACCTCAATAGTGCCCGCCGCATTCGCAATATTAGCGGTCACCGAAAGAGTGAGACAGAGAAG GAGTCTGGGCTCAAGCCAACCCTACGGCAGATTCTAAATGCATCTCGGAGAAATGTCAACTGGGAACAGG TCATTCAGCAAGTAACCAAGAAAAAGCAAGAGCTGGGCAAAGGCTTACCCAGGTTTGGCATAGAAATGGT ACCCCTTGTTCAAAATGAACAAGAAGCCTTAGATTTGGATGGGGAACCTGATCTGTCCAGTCTAGAAGGA TTCCAGTGGGAAGGTGTTTCCATTTCCTCGTCCCCTGGCTTGGCAAGAAAGCGAAGCCTTTCTGAGAGCA GCGTGATCATGGACAGAGCTCCTTCTGTGTATAGCTTCTTCAGTGAGGAAGGTACAGGCAAAGAAAATGA GCCCCAGCAGATGGTTTCACCTAGTAACTCATTGAGGGCTGGACAGAGCCAGAAAGCAACCATGCACCTC AAACAAGAAGTGACACCTCGGGCTGCCTCCCTCCGAACAGGTGAAAGGGCTGAAAATGTTGCTACCCAAA GGCGACATAGTGCACAATTATCCTCTGACCATATAATACCTTTGATGCATTTGGCAAAAGACTTGAACAG CCAGGAGAGGTCTATACCACCGTCAGAGAATCAGAATTCCCAGGAGAGTAATGGAGAGGGAAACTGTCTG TCATCAAGCGCATCCTCAGCCCTTGCGATCTCCAGTTTAGCGGATGCAGCCACAGATAGTAGCTGTACCT CTGGTGCTGAACAAAATGATGGCCAAAGTATTAGAAAGAAACGAAGAGCCACTGGAGATGGATCTTCTCC TGAACTCCCAAGTCTTGAGAGAAAAAATAAAAGAAGGAAAATTAAAGGAAAAAAAGAACGTTCTCAGGTT GACCAGCTGCTGAATATTTCTTTAAGGGAGGAAGAACTTAGTAAGTCATTGCAGTGCATGGATAACAATC TTCTGCAAGCCCGTGCAGCCCTTCAGACAGCTTATGTGGAAGTTCAGAGGCTACTTATGCTCAAGCAGCA GATAACTATGGAGATGAGTGCACTGAGGACCCATAGAATACAGATTCTACAGGGATTACAAGAAACATAT GAACCTTCTGAGCACCCAGACCAGGTTCCCTGTAGCCTCACACGAGAACGAAGGAACAGTAGATCTCAAA CATCCATTGATGCCGCACTGCTGCCCACTCCCTTTTTCCCACTTTTTCTGGAGCCTCCATCTTCCCATGT GTCTCCATCACCCACCGGAGCCTCTCTTCAAATAACCACGTCTCCTACTTTCCAAACCCATGGCAGTGTC CCTGCTCCAGACTCATCAGTTCAGATTAAACAAGAGCCCATGTCTCCTGAACAAGATGAGAATGTGAATG CTGTGCCACCAAGCTCTGCCTGCAATGTGTCCAAGGAATTACTGGAAGCTAATAGAGAGATCAGTGACAG TTGTCCAGTTTATCCAGTCATCACTGCTAGATTGTCCTTACCAGAGTCAACAGAAAGTTTCCATGAGCCT AGCCAAGAACTGAAGTTTTCTGTGGAGCAAAGAAATACCAGAAACAGAGAAAACTCTCCCTCTTCCCAAT CAGCTGGTCTTTCTAGCATAAATAAAGAAGGGGAAGAGCCAACCAAAGGCAATAGTGGGTCTGAAGCCTG TACCAGTTCTTTTCTAAGATTGTCTTTTGCTTCAGAAACCCCTTTGGAGAAGGAACCCCACTCTCCAGCT GACCAGCCTGAACAACAGGCAGAATCCACTTTGACATCAGCTGAGACTAGGGGAAGCAAGAAAAAGAAGA AACTCCGGAAGAAGAAAAGTCTACGGGCTGCCCATGTTCCTGAGAATAGTGACACTGAACAGGATGTTTT GACTGTTAAACCTGTAAGGAAAGTAAAAGCTGGAAAGTTAATTAAAGGGGGGAAAGTAACAACCTCCACT TGGGAAGACAGCAGGACTGGTCGGGAGCAGGAGAGTGTCAGAGATGAGCCAGATAGTGACTCGTCTCTGG AAGTCCTAGAAATTCCTAATCCTCAGTTAGAAGTAGTAGCCATTGATTCTTCAGAATCAGGAGAAGAGAA ACCAGACAGCCCATCTAAAAAGGATATTTGGAACTCTACAGAGCAAAACCCACTAGAAACGTCTCGTTCT GGGTGTGATGAAGTTAGCTCTACCAGTGAAATTGGCACTCGCTATAAAGATGGCATCCCTGTAAGTGTGG CAGAAACTCAGACTGTGATCTCCTCCATAAAAGGATCAAAGAATTCTTCAGAAATATCTTCAGAGCCAGG AGATGATGATGAACCCACAGAAGGAAGCTTTGAGGGACACCAAGCTGCCGTAAATGCAATTCAGATATTT GGGAACTTGCTATATACCTGTTCAGCAGATAAAACTGTTCGGGTTTATAATCTGGTGAGTCGGAAATGTA TTGGTGTCTTTGAGGGTCATACCTCCAAAGTTAACTGCCTCCTGGTTACTCAGACCTCCGGGAAGAATGC TGCCCTTTACACCGGGTCCAGTGACCATACCATCCGCTGCTATAATGTTAAGAGCCGAGAGTGTGTGGAG CAGTTACAGCTGGAAGACCGGGTCCTCTGCCTCCACAGTAGATGGCGAATCCTCTATGCGGGACTGGCAA ATGGCACTGTGGTCACCTTCAACATAAAGAACAACAAACGACTTGAGATCTTTGAATGCCATGGCCCTCG GGCAGTCAGCTGTCTTGCTACAGCTCAGGAAGGTGCCCGAAAACTGCTGGTCGTGGGGTCTTATGACTGC ACAATTAGTGTACGCGATGCCCGGAATGGACTGCTCCTCAGAACTCTGGAGGGCCATAGCAAAACCATTC TTTGCATGAAGGTGGTGAATGATCTCGTGTTCAGTGGCTCCAGTGATCAGTCAGTCCATGCTCACAACAT TCACACTGGTGAGCTCGTGCGGATCTATAAAGGTCACAATCATGCAGTGACTGTGGTGAATATCCTAGGA AAAGTGATGGTGACTGCTTGCCTGGATAAATTTGTTCGTGTCTATGAATTACAGTCTCATGATCGATTAC AAGTTTATGGAGGACACAAAGACATGATTATGTGTATGACCATCCATAAAAGCATGATTTACACTGGCTG TTATGATGGCAGTATTCAGGCCGTGAGGCTTAATCTGATGCAGAATTACCGCTGTTGGTGGCATGGTTGC TCTCTGATATTTGGCGTTGTAGATCATTTAAAACAACACTTGCTGACCGACCACACTAATCCCAACTTCC AGACTCTGAAATGTCGCTGGAAGAACTGCGATGCTTTTTTCACTGCTAGGAAAGGATCCAAACAGGATGC TGCAGGACATATTGAACGACATGCTGAAGATGACAGCAAAATTGATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC217612 representing NM_022473 Red=Cloning site Green=Tags(s) MPVGRIECPSSPSFPRDISHECRVCGVTEVGLSAYAKHISGQLHKDNVDAQEREDDGKGEEEEEDYFDKE LIQLIKQRKEQSRQDEPSNSNQEINSDDRRPQWRREDRIPYQDRESYSQPAWHHRGPPQRDWKWEKDGFN NTRKNSFPHSLRNGGGPRGRSGWHKGVAGGSSTWFHNHSNSGGGWLSNSGAVDWNHNGTGRNSSWLSEGT GGFSSWHMNNSNGNWKSSVRSTNNWNYSGPGDKFQPGRNRNSNCQMEDMTMLWNKKSNKSNKYSHDRYNW QRQENDKLGTVATYRGPSEGFTSDKFPSEGLLDFNFEQLESQTTKQADTATSKVSGKNGSAAREKPRRWT PYPSQKTLDLQSGLKDITGNKSEMIEKPLFDFSLITTGIQEPQTDETRNSPTQKTQKEIHTGSLNHKASS DSAASFEVVRQCPTAEKPEQEHTPNKMPSLKSPLLPCPATKSLSQKQDPKNISKNTKTNFFSPGEHSNPS NKPTVEDNHGPYISKLRSSCPHVLKGNKSTFGSQKQSGDNLNDTLRKAKEVLQCHESLQNPLLSTSKSTR NYAKASRNVEESEKGSLKIEFQVHALEDESDGETSDTEKHGTKIGTLGSATTELLSGSTRTADEKEEDDR ILKTSRELSTSPCNPIVRQKESELQMTSAASPHPGLLLDLKTSLEDAQVDDSIKSHVSYETEGFESASLD AELQKSDISQPSGPLLPELSKLGFPASLQRDLTRHISLKSKTGVHLPEPNLNSARRIRNISGHRKSETEK ESGLKPTLRQILNASRRNVNWEQVIQQVTKKKQELGKGLPRFGIEMVPLVQNEQEALDLDGEPDLSSLEG FQWEGVSISSSPGLARKRSLSESSVIMDRAPSVYSFFSEEGTGKENEPQQMVSPSNSLRAGQSQKATMHL KQEVTPRAASLRTGERAENVATQRRHSAQLSSDHIIPLMHLAKDLNSQERSIPPSENQNSQESNGEGNCL SSSASSALAISSLADAATDSSCTSGAEQNDGQSIRKKRRATGDGSSPELPSLERKNKRRKIKGKKERSQV DQLLNISLREEELSKSLQCMDNNLLQARAALQTAYVEVQRLLMLKQQITMEMSALRTHRIQILQGLQETY EPSEHPDQVPCSLTRERRNSRSQTSIDAALLPTPFFPLFLEPPSSHVSPSPTGASLQITTSPTFQTHGSV PAPDSSVQIKQEPMSPEQDENVNAVPPSSACNVSKELLEANREISDSCPVYPVITARLSLPESTESFHEP SQELKFSVEQRNTRNRENSPSSQSAGLSSINKEGEEPTKGNSGSEACTSSFLRLSFASETPLEKEPHSPA DQPEQQAESTLTSAETRGSKKKKKLRKKKSLRAAHVPENSDTEQDVLTVKPVRKVKAGKLIKGGKVTTST WEDSRTGREQESVRDEPDSDSSLEVLEIPNPQLEVVAIDSSESGEEKPDSPSKKDIWNSTEQNPLETSRS GCDEVSSTSEIGTRYKDGIPVSVAETQTVISSIKGSKNSSEISSEPGDDDEPTEGSFEGHQAAVNAIQIF GNLLYTCSADKTVRVYNLVSRKCIGVFEGHTSKVNCLLVTQTSGKNAALYTGSSDHTIRCYNVKSRECVE QLQLEDRVLCLHSRWRILYAGLANGTVVTFNIKNNKRLEIFECHGPRAVSCLATAQEGARKLLVVGSYDC TISVRDARNGLLLRTLEGHSKTILCMKVVNDLVFSGSSDQSVHAHNIHTGELVRIYKGHNHAVTVVNILG KVMVTACLDKFVRVYELQSHDRLQVYGGHKDMIMCMTIHKSMIYTGCYDGSIQAVRLNLMQNYRCWWHGC SLIFGVVDHLKQHLLTDHTNPNFQTLKCRWKNCDAFFTARKGSKQDAAGHIERHAEDDSKIDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022473
ORF Size	5649 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022473.3
RefSeq Size	10890 bp
RefSeq ORF	5652 bp
Locus ID	64397
UniProt ID	Q9H2Y7
Domains	WD40, zf-C2H2
MW	209.3 kDa
Gene Summary	RNA-binding protein. Specifically binds to 5'-GGGGCC-3' sequence repeats in RNA. Essential for maintenance of peripheral motor neuron and skeletal muscle function. Required for normal expression and/or alternative splicing of a number of genes in spinal cord and skeletal muscle, including the neurite outgrowth inhibitor RTN4. Also contributes to normal mitochondrial respiratory function in motor neurons, via an unknown mechanism.[UniProtKB/Swiss-Prot Function]

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