United States
Germany
Japan
United Kingdom
China
KRT28 (NM_181535) Human Tagged ORF Clone
CAT＃: RC217554
KRT28 (Myc-DDK-tagged)-Human keratin 28 (KRT28)
ORF Plasmid: tGFP
"NM_181535" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | K25IRS4; KRT25D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217554 representing NM_181535
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTCCAATTTTCTAATGGATCCAGGCATGTTTGCTTAAGGTCTGGAGCTGGATCTGTCAGACCCC TCAATGGAGGTGCAGGCTTTGCAGGCAGCAGTGCATGTGGTGGCTCTGTTGCTGGAAGTGAATTTTCCTG TGCCTTGGGAGGGGGCTTGGGCAGTGTTCCTGGTGGGAGCCATGCTGGTGGTGCCCTTGGAAATGCTGCT TGTATTGGCTTTGCTGGAAGCGAAGGGGGACTCCTCTCTGGAAATGAGAAGGTGACCATGCAAAATCTTA ATGACCGCTTGGCATCCTACCTGGATAATGTGCGAGCTCTGGAGGAGGCAAATGCTGAATTAGAGAGAAA AATCAAGGGTTGGTATGAAAAATACGGACCTGGATCTTGCCGTGGACTTGATCATGACTATAGCAGATAT CACCTAACAATTGAGGATCTTAAGAATAAGATTATCTCCTCCACTACTACTAATGCTAATGTCATTCTGC AGATTGATAATGCCAGACTGGCTGCTGATGATTTCAGGCTAAAGTATGAAAATGAGCTCACCCTTCACCA AAACGTAGAGGCCGACATCAACGGATTACGGCGAGTCCTGGACGAGCTGACGCTCTGCAGGACCGACCAG GAGCTGCAATATGAGTCTCTGAGTGAGGAGATGACATATCTCAAAAAGAACCACGAAGAGGAGATGAAGG CTCTGCAGTGCGCGGCTGGGGGCAACGTGAACGTGGAGATGAACGCGGCCCCGGGGGTAGACCTCGCGGT TTTGTTGAACAACATGCGAGCGGAGTACGAAGCCCTTGCAGAGCAGAACCGCAAGGACGCGGAGGCCTGG TTCAATGAGAAGAGCGCCTCGCTGCAGCAACAGATCTCCCACGACTCAGGCGCAGCCACTTTCGCCCGGA GCCAGCTCACCGAGATGAGGCGCACCCTGCAGACCCTGGAGATCCAGCTGCAGTCCCTGATGGCCACGAA ACACTCCCTGGAGTGCTCCTTGACAGAGACCGAGAGCAACTACTGTACGCAGCTGGCGCAGATCCAGGCT CAGATCGGGGCCCTGGAGGAGCAGCTGCACCAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGAGTATG AGCATCTCCTCGATGTCAAGGTCCACTTGGAAAAAGAAATTGAGACCTACTGCCGCCTGATAGATGGAGA TGGAAATTCATGCTCCAAATCAAAGGGCTTTGGATCAGGAAGCCCTGGGAATTCATCTAAAGATTTATCC AAAACCACACTGGTAAAGACAGTGGTTGAAGAGCTAGATCAACGTGGTAAAGTTCTTTCATCAAGGATTC ACTCCATTGAAGAAAAGACATCTAAAATGACCAACGGCAAGACAGAACAAAGGGTTCCTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217554 representing NM_181535
Red=Cloning site Green=Tags(s) MSLQFSNGSRHVCLRSGAGSVRPLNGGAGFAGSSACGGSVAGSEFSCALGGGLGSVPGGSHAGGALGNAA CIGFAGSEGGLLSGNEKVTMQNLNDRLASYLDNVRALEEANAELERKIKGWYEKYGPGSCRGLDHDYSRY HLTIEDLKNKIISSTTTNANVILQIDNARLAADDFRLKYENELTLHQNVEADINGLRRVLDELTLCRTDQ ELQYESLSEEMTYLKKNHEEEMKALQCAAGGNVNVEMNAAPGVDLAVLLNNMRAEYEALAEQNRKDAEAW FNEKSASLQQQISHDSGAATFARSQLTEMRRTLQTLEIQLQSLMATKHSLECSLTETESNYCTQLAQIQA QIGALEEQLHQVRTETEGQKLEYEHLLDVKVHLEKEIETYCRLIDGDGNSCSKSKGFGSGSPGNSSKDLS KTTLVKTVVEELDQRGKVLSSRIHSIEEKTSKMTNGKTEQRVPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181535 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181535.3, NP_853513.2 |
| RefSeq Size | 1656 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 162605 |
| UniProt ID | Q7Z3Y7 |
| MW | 50.4 kDa |
| Gene Summary | This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217554L3 | Lenti ORF clone of Human keratin 28 (KRT28), Myc-DDK-tagged |
CNY 5,990.00 |
|
| RC217554L4 | Lenti ORF clone of Human keratin 28 (KRT28), mGFP tagged |
CNY 5,990.00 |
|
| RG217554 | KRT28 (tGFP-tagged) - Human keratin 28 (KRT28) |
CNY 4,470.00 |
|
| SC307302 | KRT28 (untagged)-Human keratin 28 (KRT28) |
CNY 7,410.00 |
