United States
Germany
Japan
United Kingdom
China
TRIM68 (NM_018073) Human Tagged ORF Clone
CAT＃: RC217536
TRIM68 (Myc-DDK-tagged)-Human tripartite motif containing 68 (TRIM68)
ORF Plasmid: tGFP
"NM_018073" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GC109; RNF137; SS-56; SS56 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217536 representing NM_018073
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCACAGCCTTGGTGGAAGCCATTGTGGAAGAAGTGGCCTGTCCCATCTGTATGACCTTCCTGA GGGAGCCCATGAGCATTGACTGTGGCCACAGCTTCTGCCACAGCTGTCTCTCTGGACTCTGGGAGATCCC AGGAGAATCCCAGAACTGGGGTTACACCTGTCCCCTCTGTCGAGCTCCTGTCCAGCCAAGGAACCTGCGG CCTAATTGGCAGCTGGCCAATGTTGTAGAAAAAGTCCGTCTGCTAAGGCTACATCCAGGAATGGGGCTGA AGGGTGACCTGTGTGAGCGCCATGGGGAAAAGCTGAAGATGTTCTGCAAAGAGGATGTCTTGATAATGTG TGAGGCCTGCAGCCAGTCCCCAGAGCATGAGGCCCACAGTGTTGTGCCAATGGAGGATGTTGCCTGGGAG TACAAGTGGGAACTTCATGAGGCCCTCGAACATCTGAAGAAAGAGCAAGAAGAGGCCTGGAAGCTTGAAG TTGGTGAAAGGAAACGAACTGCCACCTGGAAGATACAGGTGGAAACCCGAAAACAGAGTATTGTATGGGA GTTTGAAAAATACCAGCGATTACTAGAGAAAAAGCAGCCACCACATCGGCAGCTGGGGGCAGAGGTAGCA GCAGCTCTGGCCAGCCTACAGCGGGAGGCAGCGGAGACCATGCAGAAACTGGAGTTGAACCATAGCGAGC TCATCCAGCAGAGCCAGGTCCTGTGGAGGATGATTGCAGAGTTGAAAGAGAGGTCGCAGAGGCCTGTCCG CTGGATGTTGCAGGATATTCAGGAAGTGTTAAACAGGAGCAAATCTTGGAGCTTGCAGCAGCCAGAACCA ATCTCCCTGGAGTTGAAGACAGATTGCCGTGTGCTGGGGCTAAGAGAGATCCTGAAGACTTATGCAGCTG ATGTGCGCTTGGATCCAGATACTGCTTACTCCCGTCTCATCGTGTCTGAGGACAGAAAACGTGTGCACTA TGGAGACACCAACCAGAAACTGCCAGACAATCCTGAGAGATTTTACCGCTATAATATCGTCCTGGGAAGC CAGTGCATCTCCTCAGGCCGGCACTACTGGGAGGTGGAGGTGGGAGACAGGTCTGAGTGGGGCCTGGGAG TATGTAAGCAAAATGTAGACCGGAAGGAGGTGGTCTACTTATCCCCCCACTATGGATTCTGGGTGATAAG GCTGAGGAAGGGAAATGAGTACCGAGCAGGCACCGATGAGTACCCAATCCTGTCCTTGCCGGTCCCTCCT CGCCGGGTGGGAATCTTCGTGGATTATGAGGCCCATGACATTTCTTTCTACAATGTGACTGACTGTGGCT CCCACATCTTCACTTTCCCCCGCTATCCCTTCCCTGGGCGCCTCCTGCCCTATTTTAGTCCTTGCTACAG CATTGGAACCAACAACACTGCTCCTCTGGCCATCTGCTCCCTGGATGGGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217536 representing NM_018073
Red=Cloning site Green=Tags(s) MDPTALVEAIVEEVACPICMTFLREPMSIDCGHSFCHSCLSGLWEIPGESQNWGYTCPLCRAPVQPRNLR PNWQLANVVEKVRLLRLHPGMGLKGDLCERHGEKLKMFCKEDVLIMCEACSQSPEHEAHSVVPMEDVAWE YKWELHEALEHLKKEQEEAWKLEVGERKRTATWKIQVETRKQSIVWEFEKYQRLLEKKQPPHRQLGAEVA AALASLQREAAETMQKLELNHSELIQQSQVLWRMIAELKERSQRPVRWMLQDIQEVLNRSKSWSLQQPEP ISLELKTDCRVLGLREILKTYAADVRLDPDTAYSRLIVSEDRKRVHYGDTNQKLPDNPERFYRYNIVLGS QCISSGRHYWEVEVGDRSEWGLGVCKQNVDRKEVVYLSPHYGFWVIRLRKGNEYRAGTDEYPILSLPVPP RRVGIFVDYEAHDISFYNVTDCGSHIFTFPRYPFPGRLLPYFSPCYSIGTNNTAPLAICSLDGED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018073 |
| ORF Size | 1455 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018073.8 |
| RefSeq Size | 3321 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 55128 |
| UniProt ID | Q6AZZ1 |
| Domains | zf-B_box, RING, SPRY |
| Protein Families | Druggable Genome |
| MW | 56.1 kDa |
| Gene Summary | This gene encodes a member of the tripartite motif-containing protein family, whose members are characterized by a "really interesting new gene" (RING) finger domain, a zinc-binding B-box motif, and a coiled-coil region. Members of this family function as E3 ubiquitin ligases and are involved in a broad range of biological processes. This gene regulates the activation of nuclear receptors, such as androgen receptor, and has been implicated in development of prostate cancer cells, where its expression increases in response to a downregulation of microRNAs. In addition, this gene participates in viral defense regulation as a negative regulator of interferon-beta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217536L1 | Lenti ORF clone of Human tripartite motif containing 68 (TRIM68), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC217536L2 | Lenti ORF clone of Human tripartite motif containing 68 (TRIM68), mGFP tagged |
CNY 5,890.00 |
|
| RC217536L3 | Lenti ORF clone of Human tripartite motif containing 68 (TRIM68), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217536L4 | Lenti ORF clone of Human tripartite motif containing 68 (TRIM68), mGFP tagged |
CNY 5,890.00 |
|
| RG217536 | TRIM68 (tGFP-tagged) - Human tripartite motif containing 68 (TRIM68) |
CNY 7,088.00 |
|
| SC108792 | TRIM68 (untagged)-Human tripartite motif containing 68 (TRIM68) |
CNY 5,856.00 |
