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ZNF521 (NM_015461) Human Tagged ORF Clone
CAT＃: RC217463
ZNF521 (Myc-DDK-tagged)-Human zinc finger protein 521 (ZNF521)
ORF Plasmid: tGFP
"NM_015461" in other vectors (6)
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CNY 9,128.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EHZF; Evi3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217463 representing NM_015461
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGACTG AAGATGGAGAGGCACTAGATTGTAAGAAGAGGCCGGAAGACGGGGAGGAGTTGGAAGACGAAGCTGTGCA CAGCTGTGACAGCTGCCTCCAGGTGTTTGAATCGCTGAGCGATATCACAGAACACAAGATTAATCAATGT CAACTGACAGATGGAGTGGATGTTGAAGATGATCCGACTTGCTCTTGGCCAGCTTCCTCACCTTCTAGCA AGGATCAGACTTCCCCTAGCCATGGAGAAGGTTGCGATTTTGGAGAGGAAGAAGGTGGCCCTGGGCTTCC ATACCCGTGTCAATTCTGTGACAAGTCGTTTAGCCGCCTCAGCTACCTAAAGCACCATGAGCAGAGTCAC AGTGACAAACTGCCTTTCAAATGCACCTACTGCAGTAGGCTGTTCAAACACAAGCGCAGCCGAGATCGCC ACATAAAACTCCACACCGGGGACAAGAAGTACCACTGCAGTGAATGTGATGCTGCGTTTTCCAGAAGTGA TCACTTGAAGATCCACTTAAAGACTCACACGTCCAACAAGCCATATAAATGTGCCATTTGTCGCCGTGGG TTTCTGTCCTCTAGTTCCTTACACGGACACATGCAGGTTCATGAGAGGAACAAGGACGGCTCTCAGTCCG GTTCCAGGATGGAGGACTGGAAGATGAAGGACACTCAGAAGTGCAGTCAGTGTGAGGAAGGCTTTGACTT CCCGGAAGACCTCCAAAAACACATTGCAGAGTGCCACCCCGAATGCTCCCCAAATGAGGACCGAGCGGCC CTCCAGTGTGTCTACTGCCACGAGCTCTTCGTAGAGGAGACCTCCCTCATGAACCACATGGAGCAGGTGC ATAGCGGGGAGAAGAAGAACTCATGCAGCATTTGTTCTGAGAGTTTCCACACAGTTGAGGAACTGTACAG CCACATGGACAGTCACCAGCAACCGGAGTCATGCAATCACAGCAACAGCCCTTCCCTGGTCACGGTGGGC TATACCTCCGTGTCCAGTACGACTCCAGATTCCAACCTCTCAGTGGACAGCTCAACCATGGTGGAAGCTG CCCCGCCAATCCCAAAGAGTCGAGGGAGGAAGAGGGCCGCTCAACAAACCCCTGACATGACTGGTCCCTC GAGTAAACAAGCAAAAGTTACCTACAGCTGTATTTACTGCAACAAACAATTATTTTCAAGTCTTGCAGTT CTGCAGATTCACCTGAAAACTATGCACTTAGATAAGCCAGAACAGGCCCATATTTGTCAGTATTGCTTGG AGGTCCTGCCCTCACTCTATAACCTAAATGAACATCTTAAGCAAGTGCATGAAGCTCAGGACCCAGGTCT GATTGTTTCTGCCATGCCTGCCATTGTCTACCAGTGTAACTTCTGTTCCGAAGTTGTCAACGACCTCAAC ACTCTTCAGGAACACATCCGATGTTCTCATGGATTTGCAAACCCTGCAGCTAAAGATAGTAATGCATTCT TTTGTCCCCATTGCTATATGGGGTTTCTCACTGACTCTTCCCTGGAAGAGCATATTAGACAGGTTCATTG TGACCTCAGTGGCTCCCGATTTGGGTCTCCAGTGCTTGGGACTCCCAAAGAACCAGTAGTAGAAGTCTAT TCTTGTTCCTATTGTACAAATTCGCCAATATTCAACAGCGTTCTTAAACTGAACAAGCATATCAAAGAGA ATCATAAAAACATTCCCTTGGCCCTGAATTATATCCACAATGGGAAGAAATCCAGGGCCTTAAGCCCCCT ATCTCCTGTGGCCATAGAGCAGACATCTCTTAAGATGATGCAGGCAGTAGGAGGTGCACCTGCACGTCCC ACTGGAGAATATATCTGTAATCAATGTGGTGCTAAGTACACATCCCTAGACAGCTTTCAGACTCACCTAA AAACTCATCTCGACACTGTGCTTCCAAAATTGACCTGTCCTCAGTGCAACAAGGAATTCCCCAACCAAGA ATCCTTGCTGAAGCATGTTACCATTCACTTTATGATCACTTCAACGTATTACATCTGTGAGAGTTGTGAC AAGCAATTCACATCAGTGGATGACCTTCAGAAACACCTGCTGGACATGCACACCTTTGTCTTCTTTCGCT GCACCCTCTGCCAGGAAGTTTTTGACTCAAAAGTCTCCATTCAGCTCCACTTGGCTGTGAAGCACAGTAA CGAAAAGAAAGTCTATAGGTGCACATCTTGCAACTGGGACTTCCGCAACGAAACTGACTTGCAGCTCCAT GTGAAACACAACCACCTGGAAAACCAAGGGAAAGTGCATAAGTGCATTTTCTGCGGTGAGTCCTTTGGCA CCGAGGTGGAGCTGCAATGCCACATCACCACTCACAGTAAGAAGTACAACTGCAAGTTCTGTAGCAAAGC CTTCCATGCGATCATTTTGTTAGAAAAACACTTGCGAGAAAAACACTGTGTATTCGAAACCAAGACACCC AACTGTGGAACAAATGGAGCTTCCGAGCAAGTGCAGAAAGAGGAAGTGGAGCTGCAGACTTTGCTGACCA ACAGCCAGGAGTCCCACAACAGTCACGATGGGAGCGAAGAAGACGTTGACACCTCTGAGCCTATGTACGG CTGCGACATTTGTGGGGCAGCCTACACTATGGAAACTTTGCTGCAGAATCACCAGCTCCGAGACCACAAC ATCAGACCTGGAGAAAGTGCCATCGTGAAAAAGAAAGCTGAGCTCATTAAAGGGAATTACAAGTGCAACG TGTGCTCTCGAACCTTCTTCTCCGAAAATGGCCTCCGGGAACATATGCAGACCCACCTAGGCCCTGTCAA ACACTACATGTGCCCTATTTGCGGAGAGCGGTTTCCCTCCCTTTTAACTCTTACTGAACACAAAGTCACG CATAGTAAGAGTCTTGATACTGGAAACTGCCGGATTTGCAAGATGCCTCTCCAGAGTGAAGAGGAGTTTT TAGAGCATTGCCAAATGCACCCTGACTTGAGGAATTCCCTGACAGGCTTTCGCTGCGTGGTGTGCATGCA GACAGTGACCTCCACCTTGGAACTCAAAATCCATGGGACGTTCCACATGCAAAAGACAGGGAATGGGTCT GCAGTTCAGACCACAGGGCGGGGCCAGCACGTCCAAAAACTGTATAAGTGCGCATCTTGCCTCAAAGAAT TCCGTTCCAAGCAAGATCTGGTGAAACTTGATATCAATGGCCTGCCATATGGTCTGTGTGCCGGCTGCGT GAATCTCAGTAAGAGCGCCAGCCCAGGCATTAACGTCCCTCCCGGCACGAATAGACCAGGCTTGGGCCAG AATGAGAATCTGAGTGCCATTGAGGGGAAAGGCAAGGTGGGGGGACTGAAGACACGCTGCTCTAGCTGCA ACGTTAAGTTTGAGTCTGAAAGTGAACTCCAGAACCACATCCAAACCATCCACCGAGAGCTCGTGCCAGA CAGCAACAGCACACAGTTGAAAACGCCCCAAGTATCACCAATGCCCAGAATCAGTCCCTCCCAGTCGGAT GAGAAGAAGACCTATCAATGCATCAAGTGTCAGATGGTTTTCTACAATGAATGGGATATTCAGGTTCATG TTGCAAATCACATGATTGATGAAGGACTGAACCATGAATGCAAACTCTGCAGCCAGACCTTTGACTCTCC TGCCAAACTCCAGTGCCACCTGATAGAGCACAGCTTCGAAGGGATGGGAGGCACCTTCAAGTGTCCAGTC TGCTTTACAGTATTTGTTCAAGCAAACAAGTTGCAGCAGCATATTTTCTCTGCCCATGGACAAGAAGACA AGATCTATGACTGTACACAATGTCCACAGAAGTTTTTCTTCCAAACAGAGCTGCAGAATCATACAATGAC CCAACACAGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217463 representing NM_015461
Red=Cloning site Green=Tags(s) MSRRKQAKPRSLKDPNCKLEDKTEDGEALDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQC QLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSH SDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRG FLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAA LQCVYCHELFVEETSLMNHMEQVHSGEKKNSCSICSESFHTVEELYSHMDSHQQPESCNHSNSPSLVTVG YTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTGPSSKQAKVTYSCIYCNKQLFSSLAV LQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLN TLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVY SCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARP TGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCD KQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLH VKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTP NCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHN IRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVT HSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGS AVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGINVPPGTNRPGLGQ NENLSAIEGKGKVGGLKTRCSSCNVKFESESELQNHIQTIHRELVPDSNSTQLKTPQVSPMPRISPSQSD EKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPV CFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015461 |
| ORF Size | 3933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015461.3 |
| RefSeq Size | 4869 bp |
| RefSeq ORF | 3936 bp |
| Locus ID | 25925 |
| UniProt ID | Q96K83 |
| Domains | zf-C2H2 |
| MW | 147.7 kDa |
| Gene Summary | Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The stem cell-associated transcription co-factor, ZNF521, interacts with GLI1 and GLI2 and enhances the activity of the Sonic hedgehog pathway
,Scicchitano, S;Giordano, M;Lucchino, V;Montalcini, Y;Chiarella, E;Aloisio, A;Codispoti, B;Zoppoli, P;Melocchi, V;Bianchi, F;De Smaele, E;Mesuraca, M;Morrone, G;Bond, HM;,
Cell Death Dis
,PubMed ID 31558698
[ZNF521]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217463L1 | Lenti ORF clone of Human zinc finger protein 521 (ZNF521), Myc-DDK-tagged |
CNY 11,528.00 |
|
| RC217463L2 | Lenti ORF clone of Human zinc finger protein 521 (ZNF521), mGFP tagged |
CNY 7,600.00 |
|
| RC217463L3 | Lenti ORF clone of Human zinc finger protein 521 (ZNF521), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC217463L4 | Lenti ORF clone of Human zinc finger protein 521 (ZNF521), mGFP tagged |
CNY 11,528.00 |
|
| RG217463 | ZNF521 (tGFP-tagged) - Human zinc finger protein 521 (ZNF521) |
CNY 10,728.00 |
|
| SC127365 | ZNF521 (untagged)-Human zinc finger protein 521 (ZNF521) |
CNY 9,136.00 |
