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NPFF1 Receptor (NPFFR1) (NM_022146) Human Tagged ORF Clone
CAT＃: RC217439
NPFFR1 (Myc-DDK-tagged)-Human neuropeptide FF receptor 1 (NPFFR1)
ORF Plasmid: tGFP
"NM_022146" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR147; NPFF1; NPFF1R1; OT7T022 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217439 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACTAACACTGAGG CCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCCCCTGTGGCGGCCATGTTCAT TGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGCAACACCCTGGTCTGTTTCATCGTGCTCAAG AACCGGCACATGCATACTGTCACCAACATGTTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCA TCTTCTGCATGCCCACCACCCTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAA GATGAGCGGCTTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTCACCATCGCCG TCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTGACCGTCACCCGTGAGGAGCA CCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCTCTCTACTCCTGCTGGGAGGCCTGGCCCGAGAAG GGCATGCGCAGGGTCTACACCACTGTGCTCTTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGG TCATGTACGCCCGCATCGCGCGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGC GGACCCGCGAGCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCGCCGCAGCTGC ACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTCAACAGCAGCGCCAACCCCAT CATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTCCAGGCCGCCTTCCGCGCCCGCCTCTGCCCG CGCCCGTCGGGGAGCCACAAGGAGGCCTACTCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCG TGGTGGTGCGGCCCAGCGACTCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGG CCGCCTCCCGCTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC CTGCCCCTCACCATTCCAGCCTGGGATATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217439 protein sequence
Red=Cloning site Green=Tags(s) MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLK NRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVE RFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEK GMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFT LSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCP RPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSH LPLTIPAWDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022146 |
| ORF Size | 1292 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022146.5 |
| RefSeq Size | 1311 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 64106 |
| UniProt ID | Q9GZQ6 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 47.8 kDa |
| Gene Summary | Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217439L1 | Lenti ORF clone of Human neuropeptide FF receptor 1 (NPFFR1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC217439L2 | Lenti ORF clone of Human neuropeptide FF receptor 1 (NPFFR1), mGFP tagged |
CNY 5,890.00 |
|
| RC217439L3 | Lenti ORF clone of Human neuropeptide FF receptor 1 (NPFFR1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217439L4 | Lenti ORF clone of Human neuropeptide FF receptor 1 (NPFFR1), mGFP tagged |
CNY 5,890.00 |
|
| RG217439 | NPFFR1 (tGFP-tagged) - Human neuropeptide FF receptor 1 (NPFFR1) |
CNY 5,256.00 |
|
| SC305005 | NPFFR1 (untagged)-Human neuropeptide FF receptor 1 (NPFFR1) |
CNY 3,656.00 |
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