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Metabotropic Glutamate Receptor 7 (GRM7) (NM_000844) Human Tagged ORF Clone
CAT＃: RC217402
GRM7 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1
ORF Plasmid: tGFP
"NM_000844" in other vectors (6)
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CNY 6,704.00
CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GLUR7; GPRC1G; MGLU7; MGLUR7; NEDSHBA; PPP1R87 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217402 representing NM_000844
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCAGCTGAGGAAGCTGCTCCGCGTCCTGACTTTGATGAAGTTCCCCTGCTGCGTGCTGGAGGTGC TCCTGTGCGCGCTGGCGGCGGCGGCGCGCGGCCAGGAGATGTACGCCCCGCACTCAATCCGGATCGAGGG GGACGTCACCCTCGGGGGGCTGTTCCCCGTGCACGCCAAGGGTCCCAGCGGAGTGCCCTGCGGCGACATC AAGAGGGAAAACGGGATCCACAGGCTGGAAGCGATGCTCTACGCCCTGGACCAGATCAACAGTGATCCCA ACCTACTGCCCAACGTGACGCTGGGCGCGCGGATCCTGGACACTTGTTCCAGGGACACTTACGCGCTCGA ACAGTCGCTTACTTTCGTCCAGGCGCTCATCCAGAAGGACACCTCCGACGTGCGCTGCACCAACGGCGAA CCGCCGGTTTTCGTCAAGCCGGAGAAAGTAGTTGGAGTGATTGGGGCTTCGGGGAGTTCGGTCTCCATCA TGGTAGCCAACATCCTGAGGCTCTTCCAGATCCCCCAGATTAGTTATGCATCAACGGCACCCGAGCTAAG TGATGACCGGCGCTATGACTTCTTCTCTCGCGTGGTGCCACCCGATTCCTTCCAAGCCCAGGCCATGGTA GACATTGTAAAGGCCCTAGGCTGGAATTATGTGTCTACCCTCGCATCGGAAGGAAGTTATGGAGAGAAAG GTGTGGAGTCCTTCACGCAGATTTCCAAAGAGGCAGGTGGACTCTGCATTGCCCAGTCCGTGAGAATCCC CCAGGAACGCAAAGACAGGACCATTGACTTTGATAGAATTATCAAACAGCTCCTGGACACCCCCAACTCC AGGGCCGTCGTGATTTTTGCCAACGATGAGGATATAAAGCAGATCCTTGCAGCAGCCAAAAGAGCTGACC AAGTTGGCCATTTTCTTTGGGTGGGATCAGACAGCTGGGGATCCAAAATAAACCCACTGCACCAGCATGA AGATATCGCAGAAGGGGCCATCACCATTCAGCCCAAGCGAGCCACGGTGGAAGGGTTTGATGCCTACTTT ACGTCCCGTACACTTGAAAACAACAGAAGAAATGTATGGTTTGCCGAATACTGGGAGGAAAACTTCAACT GCAAGTTGACGATTAGTGGGTCAAAAAAAGAAGACACAGATCGCAAATGCACAGGACAGGAGAGAATTGG AAAAGATTCCAACTATGAGCAGGAGGGTAAAGTCCAGTTCGTGATTGACGCAGTCTATGCTATGGCTCAC GCCCTTCACCACATGAACAAGGATCTCTGTGCTGACTACCGGGGTGTCTGCCCAGAGATGGAGCAAGCTG GAGGCAAGAAGTTGCTGAAGTATATACGCAATGTTAATTTCAATGGTAGTGCTGGCACTCCAGTGATGTT TAACAAGAACGGGGATGCACCTGGGCGTTATGACATCTTTCAGTACCAGACCACAAACACCAGCAACCCG GGTTACCGTCTGATCGGGCAGTGGACAGACGAACTTCAGCTCAATATAGAAGACATGCAGTGGGGTAAAG GAGTCCGAGAGATACCCGCCTCAGTGTGCACACTACCATGTAAGCCAGGACAGAGAAAGAAGACACAGAA AGGAACTCCTTGCTGTTGGACCTGTGAGCCTTGCGATGGTTACCAGTACCAGTTTGATGAGATGACATGC CAGCATTGCCCCTATGACCAGAGGCCCAATGAAAATCGAACCGGATGCCAGGATATTCCCATCATCAAAC TGGAGTGGCACTCCCCCTGGGCTGTGATTCCTGTCTTCCTGGCAATGTTGGGGATCATTGCCACCATCTT TGTCATGGCCACTTTCATCCGCTACAATGACACGCCCATTGTCCGGGCATCTGGGCGGGAACTCAGCTAT GTTCTTTTGACGGGCATCTTTCTTTGCTACATCATCACTTTCCTGATGATTGCCAAACCAGATGTGGCAG TGTGTTCTTTCCGGCGAGTTTTCTTGGGCTTGGGTATGTGCATCAGTTATGCAGCCCTCTTGACGAAAAC AAATCGGATTTATCGCATATTTGAGCAGGGCAAGAAATCAGTAACAGCTCCCAGACTCATAAGCCCAACA TCACAACTGGCAATCACTTCCAGTTTAATATCAGTTCAGCTTCTAGGGGTGTTCATTTGGTTTGGTGTTG ATCCACCCAACATCATCATAGACTATGATGAACACAAGACAATGAACCCTGAGCAAGCCAGAGGGGTTCT CAAGTGTGACATTACAGATCTCCAAATCATTTGCTCCTTGGGATATAGCATTCTTCTCATGGTCACATGT ACTGTGTATGCCATCAAGACTCGGGGTGTACCCGAGAATTTTAACGAAGCCAAGCCCATTGGATTCACTA TGTACACGACATGTATAGTATGGCTTGCCTTCATTCCAATTTTTTTTGGCACCGCTCAATCAGCGGAAAA GCTCTACATACAAACTACCACGCTTACAATCTCCATGAACCTAAGTGCATCAGTGGCGCTGGGGATGCTA TACATGCCGAAAGTGTACATCATCATTTTCCACCCTGAACTCAATGTCCAGAAACGGAAGCGAAGCTTCA AGGCGGTAGTCACAGCAGCCACCATGTCATCGAGGCTGTCACACAAACCCAGTGACAGACCCAACGGTGA GGCAAAGACCGAGCTCTGTGAAAACGTAGACCCAAACAGCCCTGCTGCAAAAAAGAAGTATGTCAGTTAT AATAACCTGGTTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217402 representing NM_000844
Red=Cloning site Green=Tags(s) MVQLRKLLRVLTLMKFPCCVLEVLLCALAAAARGQEMYAPHSIRIEGDVTLGGLFPVHAKGPSGVPCGDI KRENGIHRLEAMLYALDQINSDPNLLPNVTLGARILDTCSRDTYALEQSLTFVQALIQKDTSDVRCTNGE PPVFVKPEKVVGVIGASGSSVSIMVANILRLFQIPQISYASTAPELSDDRRYDFFSRVVPPDSFQAQAMV DIVKALGWNYVSTLASEGSYGEKGVESFTQISKEAGGLCIAQSVRIPQERKDRTIDFDRIIKQLLDTPNS RAVVIFANDEDIKQILAAAKRADQVGHFLWVGSDSWGSKINPLHQHEDIAEGAITIQPKRATVEGFDAYF TSRTLENNRRNVWFAEYWEENFNCKLTISGSKKEDTDRKCTGQERIGKDSNYEQEGKVQFVIDAVYAMAH ALHHMNKDLCADYRGVCPEMEQAGGKKLLKYIRNVNFNGSAGTPVMFNKNGDAPGRYDIFQYQTTNTSNP GYRLIGQWTDELQLNIEDMQWGKGVREIPASVCTLPCKPGQRKKTQKGTPCCWTCEPCDGYQYQFDEMTC QHCPYDQRPNENRTGCQDIPIIKLEWHSPWAVIPVFLAMLGIIATIFVMATFIRYNDTPIVRASGRELSY VLLTGIFLCYIITFLMIAKPDVAVCSFRRVFLGLGMCISYAALLTKTNRIYRIFEQGKKSVTAPRLISPT SQLAITSSLISVQLLGVFIWFGVDPPNIIIDYDEHKTMNPEQARGVLKCDITDLQIICSLGYSILLMVTC TVYAIKTRGVPENFNEAKPIGFTMYTTCIVWLAFIPIFFGTAQSAEKLYIQTTTLTISMNLSASVALGML YMPKVYIIIFHPELNVQKRKRSFKAVVTAATMSSRLSHKPSDRPNGEAKTELCENVDPNSPAAKKKYVSY NNLVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000844 |
| ORF Size | 2745 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000844.4 |
| RefSeq Size | 4147 bp |
| RefSeq ORF | 2748 bp |
| Locus ID | 2917 |
| UniProt ID | Q14831 |
| Domains | 7tm_3, ANF_receptor |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 102.7 kDa |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217402L1 | Lenti ORF clone of Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1, Myc-DDK-tagged |
CNY 9,104.00 |
|
| RC217402L2 | Lenti ORF clone of Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RC217402L3 | Lenti ORF clone of Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC217402L4 | Lenti ORF clone of Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG217402 | GRM7 (tGFP-tagged) - Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1 |
CNY 8,304.00 |
|
| SC119649 | GRM7 (untagged)-Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 1 |
CNY 6,712.00 |
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