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Cyclin T1 (CCNT1) (NM_001240) Human Tagged ORF Clone
CAT＃: RC217377
- TrueORF®
CCNT1 (Myc-DDK-tagged)-Human cyclin T1 (CCNT1). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001240" in other vectors (4)
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CNY 5,320.00
CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCNT; CYCT1; HIVE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217377 ORF sequence, codon optimized.
Due to the complexity of NM_001240, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGAGAGAGGAAGAACAACAACAAACGGTGGTATTTCACTCGAGAACAGCTGGAAAATAGCCCAT CCCGTCGTTTTGGCGTGGACCCAGATAAAGAACTTTCTTATCGCCAGCAGGCGGCCAATCTGCTTCAGGA CATGGGGCAGCGTCTTAACGTCTCACAATTGACTATCAACACTGCTATAGTATACATGCATCGATTCTAC ATGATTCAGTCCTTCACACAGTTCCCTGGAAATTCTGTGGCTCCAGCAGCCTTGTTTCTAGCAGCTAAAG TGGAGGAGCAGCCCAAAAAATTGGAACATGTCATCAAGGTAGCACATACTTGTCTCCATCCTCAGGAATC CCTTCCTGATACTAGAAGTGAGGCTTATTTGCAACAAGTTCAAGATCTGGTCATTTTAGAAAGCATAATT TTGCAGACTTTAGGCTTTGAACTAACAATTGATCACCCACATACTCATGTAGTAAAGTGCACTCAACTTG TTCGAGCAAGCAAGGACTTAGCACAGACTTCTTACTTCATGGCAACCAACAGCCTGCATTTGACCACATT TAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCTGCATTCACCTGGCTTGCAAGTGGTCCAATTGG GAGATCCCAGTCTCAACTGACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACTTT TAGATGAACTGACACATGAGTTTCTACAGATTTTGGAGAAAACTCCCAACAGGCTCAAACGCATTTGGAA TTGGAGGGCATGCGAGGCTGCCAAGAAAACAAAAGCAGATGACCGAGGAACAGATGAAAAGACTTCAGAG CAGACAATCCTCAATATGATTTCCCAGAGCTCTTCAGACACAACCATTGCAGGTTTAATGAGCATGTCAA CTTCTACCACAAGTGCAGTGCCTTCCCTGCCAGTCTCCGAAGAGTCATCCAGCAACTTAACCAGTGTGGA GATGTTGCCGGGCAAGCGTTGGCTGTCCTCCCAACCTTCTTTCAAACTAGAACCTACTCAGGGTCATCGG ACTAGTGAGAATTTAGCACTTACAGGAGTTGATCATTCCTTACCCCAAGACGGCAGCAATGCGTTCATTT CCCAAAAGCAAAATAGTAAATCCGTGCCCAGCGCCAAGGTGAGCCTCAAAGAATATAGGGCGAAGCATGC AGAGGAGCTCGCCGCCCAGAAGCGCCAATTGGAGAATATGGAGGCTAACGTGAAATCTCAATACGCTTAC GCCGCACAGAACCTGCTGAGCCATCATGACAGCCACTCAAGCGTCATCCTGAAAATGCCCATAGAGGGCA GTGAAAACCCTGAGAGGCCGTTCCTGGAAAAAGCTGATAAAACAGCTCTTAAAATGAGGATCCCAGTGGC AGGGGGCGACAAGGCTGCCTCAAGTAAACCGGAAGAAATTAAAATGAGAATAAAAGTTCATGCTGCCGCC GATAAGCATAACTCTGTGGAGGATTCCGTGACTAAAAGTAGGGAGCACAAGGAAAAACACAAAACTCATC CATCAAATCATCACCACCACCACAACCACCATTCCCATAAGCACTCCCACTCCCAGCTCCCAGTGGGAAC GGGGAATAAACGCCCCGGTGACCCCAAGCACTCTAGTCAGACCAGCAACTTGGCTCATAAAACGTATTCA CTGAGCAGCTCCTTCTCTTCATCATCCAGCACCCGAAAGCGGGGCCCTTCCGAAGAGACAGGCGGGGCGG TGTTTGATCATCCAGCAAAAATCGCCAAATCTACCAAAAGCTCTTCCCTTAATTTCTCATTTCCTAGTCT GCCCACAATGGGCCAGATGCCTGGACATTCATCCGATACATCTGGCTTGTCTTTTAGCCAGCCATCCTGT AAAACTAGGGTGCCTCACTCCAAACTGGACAAAGGGCCCACCGGGGCTAATGGCCACAACACAACTCAGA CAATTGACTACCAGGATACCGTTAATATGCTGCATTCACTGCTCAGCGCCCAAGGTGTCCAGCCTACGCA GCCTACAGCTTTCGAGTTTGTGCGCCCCTACTCCGACTACCTTAATCCACGGAGCGGTGGCATCAGCTCC CGATCTGGTAATACGGATAAGCCCCGGCCCCCCCCTCTGCCATCAGAGCCACCCCCCCCACTGCCACCAC TGCCAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217377 representing NM_001240
Red=Cloning site Green=Tags(s) MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFY MIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESII LQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNW EIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSE QTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHR TSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAY AAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAA DKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYS LSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSC KTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISS RSGNTDKPRPPPLPSEPPPPLPPLPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001240 |
| ORF Size | 2178 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001240.1, NM_001240.2, NM_001240.3, NP_001231.2 |
| RefSeq Size | 6788 bp |
| RefSeq ORF | 2181 bp |
| Locus ID | 904 |
| UniProt ID | O60563 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 80.7 kDa |
| Gene Summary | This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217377L3 | Lenti-ORF clone of CCNT1 (Myc-DDK-tagged)-Human cyclin T1 (CCNT1). Note: ORF is codon optimized |
CNY 9,600.00 |
|
| RC217377L4 | Lenti-ORF clone of CCNT1 (mGFP-tagged)-Human cyclin T1 (CCNT1). Note: ORF is codon optimized |
CNY 9,600.00 |
|
| RG217377 | CCNT1 (tGFP-tagged) - Human cyclin T1 (CCNT1). Note: ORF is codon optimized |
CNY 8,460.00 |
|
| SC125253 | CCNT1 (untagged)-Human cyclin T1 (CCNT1) |
CNY 5,320.00 |
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