CACNA1B (NM_000718) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BIII; CACNL1A5; CACNN; Cav2.2; DYT23; NEDNEH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC217170 representing NM_000718. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTGGGCGGCCGCTATGGGGGCCCCGGCGGCGGAGAGCGGGCCCGGGGC GGCGGGGCCGGCGGGGCGGGGGGCCCGGGTCCCGGGGGGCTGCAGCCCGGCCAGCGGGTCCTCTACAAG CAATCGATCGCGCAGCGCGCGCGGACCATGGCGCTGTACAACCCCATCCCGGTCAAGCAGAACTGCTTC ACCGTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCGAAGCGCATCACC GAGTGGCCTCCATTCGAGTATATGATCCTGGCCACCATCATCGCCAACTGCATCGTGCTGGCCCTGGAG CAGCACCTCCCTGATGGGGACAAAACGCCCATGTCCGAGCGGCTGGACGACACGGAGCCCTATTTCATC GGGATCTTTTGCTTCGAGGCAGGGATCAAAATCATCGCTCTGGGCTTTGTCTTCCACAAGGGCTCTTAC CTGCGGAACGGCTGGAACGTCATGGACTTCGTGGTCGTCCTCACAGGGATCCTTGCCACGGCTGGAACT GACTTCGACCTGCGAACACTGAGGGCTGTGCGTGTGCTGAGGCCCCTGAAGCTGGTGTCTGGGATTCCA AGTTTGCAGGTGGTGCTCAAGTCCATCATGAAGGCCATGGTTCCACTCCTGCAGATTGGGCTGCTTCTC TTCTTTGCCATCCTCATGTTTGCCATCATTGGCCTGGAGTTCTACATGGGCAAGTTCCACAAGGCCTGT TTCCCCAACAGCACAGATGCGGAGCCCGTGGGTGACTTCCCCTGTGGCAAGGAGGCCCCAGCCCGGCTG TGCGAGGGCGACACTGAGTGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAACTTTGACAAT ATCCTGTTTGCCATCTTGACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACTGACATCCTCTATAAT ACAAACGATGCGGCCGGCAACACCTGGAACTGGCTCTACTTCATCCCTCTCATCATCATCGGCTCCTTC TTCATGCTCAACCTGGTGCTGGGCGTGCTCTCGGGGGAGTTTGCCAAGGAGCGAGAGAGGGTGGAGAAC CGCCGCGCCTTCCTGAAGCTGCGCCGGCAGCAGCAGATCGAGCGAGAGCTCAACGGGTACCTGGAGTGG ATCTTCAAGGCGGAGGAAGTCATGCTGGCCGAGGAGGACAGGAATGCAGAGGAGAAGTCCCCTTTGGAC GTGCTGAAGAGAGCGGCCACCAAGAAGAGCAGAAATGACCTGATCCACGCAGAGGAGGGAGAGGACCGG TTTGCAGATCTCTGTGCTGTTGGATCCCCCTTCGCCCGCGCCAGCCTCAAGAGCGGGAAGACAGAGAGC TCGTCATACTTCCGGAGGAAGGAGAAGATGTTCCGGTTTTTTATCCGGCGCATGGTGAAGGCTCAGAGC TTCTACTGGGTGGTGCTGTGCGTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCATTACAACCAG CCGCGGCGGCTTACCACGACCCTGTATTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATG TCCCTGAAGATGTATGGCCTGGGGCCCAGAAGCTACTTCCGGTCCTCCTTCAACTGCTTCGACTTTGGG GTCATCGTGGGGAGCGTCTTTGAAGTGGTCTGGGCGGCCATCAAGCCGGGAAGCTCCTTTGGGATCAGT GTGCTGCGGGCCCTCCGCCTGCTGAGGATCTTCAAAGTCACGAAGTACTGGAGCTCCCTGCGGAACCTG GTGGTGTCCCTGCTGAACTCCATGAAGTCCATCATCAGCCTGCTCTTCTTGCTCTTCCTGTTCATTGTG GTCTTCGCCCTGCTGGGGATGCAGCTGTTTGGGGGACAGTTCAACTTCCAGGATGAGACTCCCACAACC AACTTCGACACCTTCCCTGCCGCCATCCTCACTGTCTTCCAGATCCTGACGGGAGAGGACTGGAATGCA GTGATGTATCACGGGATCGAATCGCAAGGCGGCGTCAGCAAAGGCATGTTCTCGTCCTTTTACTTCATT GTCCTGACACTGTTCGGAAACTACACTCTGCTGAATGTCTTTCTGGCCATCGCTGTGGACAACCTGGCC AACGCCCAAGAGCTGACCAAGGATGAAGAGGAGATGGAAGAAGCAGCCAATCAGAAGCTTGCTCTGCAA AAGGCCAAAGAAGTGGCTGAAGTCAGCCCCATGTCTGCCGCGAACATCTCCATCGCCGCCAGGCAGCAG AACTCGGCCAAGGCGCGCTCGGTGTGGGAGCAGCGGGCCAGCCAGCTACGGCTGCAGAACCTGCGGGCC AGCTGCGAGGCGCTGTACAGCGAGATGGACCCCGAGGAGCGGCTGCGCTTCGCCACTACGCGCCACCTG CGGCCCGACATGAAGACGCACCTGGACCGGCCGCTGGTGGTGGAGCTGGGCCGCGACGGCGCGCGGGGG CCCGTGGGAGGCAAAGCCCGACCTGAGGCTGCGGAGGCCCCCGAGGGCGTCGACCCTCCGCGCAGGCAC CACCGGCACCGCGACAAGGACAAGACCCCCGCGGCGGGGGACCAGGACCGAGCAGAGGCCCCGAAGGCG GAGAGCGGGGAGCCCGGTGCCCGGGAGGAGCGGCCGCGGCCGCACCGCAGCCACAGCAAGGAGGCCGCG GGGCCCCCGGAGGCGCGGAGCGAGCGCGGCCGAGGCCCAGGCCCCGAGGGCGGCCGGCGGCACCACCGG CGCGGCTCCCCGGAGGAGGCGGCCGAGCGGGAGCCCCGACGCCACCGCGCGCACCGGCACCAGGATCCG AGCAAGGAGTGCGCCGGCGCCAAGGGCGAGCGGCGCGCGCGGCACCGCGGCGGCCCCCGAGCGGGGCCC CGGGAGGCGGAGAGCGGGGAGGAGCCGGCGCGGCGGCACCGGGCCCGGCACAAGGCGCAGCCTGCTCAC GAGGCTGTGGAGAAGGAGACCACGGAGAAGGAGGCCACGGAGAAGGAGGCTGAGATAGTGGAAGCCGAC AAGGAAAAGGAGCTCCGGAACCACCAGCCCCGGGAGCCACACTGTGACCTGGAGACCAGTGGGACTGTG ACTGTGGGTCCCATGCACACACTGCCCAGCACCTGTCTCCAGAAGGTGGAGGAACAGCCAGAGGATGCA GACAATCAGCGGAACGTCACTCGCATGGGCAGTCAGCCCCCAGACCCGAACACTATTGTACATATCCCA GTGATGCTGACGGGCCCTCTTGGGGAAGCCACGGTCGTTCCCAGTGGTAACGTGGACCTGGAAAGCCAA GCAGAGGGGAAGAAGGAGGTGGAAGCGGATGACGTGATGAGGAGCGGCCCCCGGCCTATCGTCCCATAC AGCTCCATGTTCTGTTTAAGCCCCACCAACCTGCTCCGCCGCTTCTGCCACTACATCGTGACCATGAGG TACTTCGAGGTGGTCATTCTCGTGGTCATCGCCTTGAGCAGCATCGCCCTGGCTGCTGAGGACCCAGTG CGCACAGACTCGCCCAGGAACAACGCTCTGAAATACCTGGATTACATTTTCACTGGTGTCTTTACCTTT GAGATGGTGATAAAGATGATCGACTTGGGACTGCTGCTTCACCCTGGAGCCTATTTCCGGGACTTGTGG AACATTCTGGACTTCATTGTGGTCAGTGGCGCCCTGGTGGCGTTTGCTTTCTCAGGATCCAAAGGGAAA GACATCAATACCATCAAGTCTCTGAGAGTCCTTCGTGTCCTGCGGCCCCTCAAGACCATCAAACGGCTG CCCAAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCCCTGAAGAATGTCCTCAACATCTTGATTGTC TACATGCTCTTCATGTTCATATTTGCCGTCATTGCGGTGCAGCTCTTCAAAGGGAAGTTTTTCTACTGC ACAGATGAATCCAAGGAGCTGGAGAGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAGGAAGTG GAAGCTCAGCCCAGGCAGTGGAAGAAATACGACTTTCACTACGACAATGTGCTCTGGGCTCTGCTGACG CTGTTCACAGTGTCCACGGGAGAAGGCTGGCCCATGGTGCTGAAACACTCCGTGGATGCCACCTATGAG GAGCAGGGTCCAAGCCCTGGGTACCGCATGGAGCTGTCCATCTTCTACGTGGTCTACTTTGTGGTCTTT CCCTTCTTCTTCGTCAACATCTTTGTGGCTTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTG ATGTCTGAATGCAGCCTGGAGAAGAACGAGAGGGCTTGCATTGACTTCGCCATCAGCGCCAAACCCCTG ACACGGTACATGCCCCAAAACCGGCAGTCGTTCCAGTATAAGACGTGGACATTTGTGGTCTCCCCGCCC TTTGAATACTTCATCATGGCCATGATAGCCCTCAACACTGTGGTGCTGATGATGAAGTTCTATGATGCA CCCTATGAGTACGAGCTGATGCTGAAATGCCTGAACATCGTGTTCACATCCATGTTCTCCATGGAATGC GTGCTGAAGATCATCGCCTTTGGGGTGCTGAACTATTTCAGAGATGCCTGGAATGTCTTTGACTTTGTC ACTGTGTTGGGAAGTATTACTGATATTTTAGTAACAGAGATTGCGGAAACGAACAATTTCATCAACCTC AGCTTCCTCCGCCTCTTTCGAGCTGCGCGGCTGATCAAGCTGCTCCGCCAGGGCTACACCATCCGCATC CTGCTGTGGACCTTTGTCCAGTCCTTCAAGGCCCTGCCCTACGTGTGTCTGCTCATTGCCATGCTGTTC TTCATCTACGCCATCATCGGCATGCAGGTGTTTGGGAATATTGCCCTGGATGATGACACCAGCATCAAC CGCCACAACAACTTCCGGACGTTTTTGCAAGCCCTGATGCTGCTGTTCAGGAGCGCCACGGGGGAGGCC TGGCACGAGATCATGCTGTCCTGCCTGAGCAACCAGGCCTGTGATGAGCAGGCCAATGCCACCGAGTGT GGAAGTGACTTTGCCTACTTCTACTTCGTCTCCTTCATCTTCCTGTGCTCCTTTCTGATGTTGAACCTC TTTGTGGCTGTGATCATGGACAATTTTGAGTACCTCACGCGGGACTCTTCCATCCTAGGTCCTCACCAC TTGGATGAGTTCATCCGGGTCTGGGCTGAATACGACCCGGCTGCGTGTGGGCGCATCAGTTACAATGAC ATGTTTGAGATGCTGAAACACATGTCCCCGCCTCTGGGGCTGGGGAAGAAATGCCCTGCTCGAGTTGCT TACAAGCGCCTGGTTCGCATGAACATGCCCATCTCCAACGAGGACATGACTGTTCACTTCACGTCCACG CTGATGGCCCTCATCCGGACGGCACTGGAGATCAAGCTGGCCCCAGCTGGGACAAAGCAGCATCAGTGT GACGCGGAGTTGAGGAAGGAGATTTCCGTTGTGTGGGCCAATCTGCCCCAGAAGACTTTGGACTTGCTG GTACCACCCCATAAGCCTGATGAGATGACAGTGGGGAAGGTTTATGCAGCTCTGATGATATTCGACTTC TACAAGCAGAACAAAACCACCAGAGACCAGATGCAGCAGGCTCCTGGAGGCCTCTCCCAGATGGGTCCT GTGTCCCTGTTCCACCCTCTGAAGGCCACCCTGGAGCAGACACAGCCGGCTGTGCTCCGAGGAGCCCGG GTTTTCCTTCGACAGAAGAGTTCCACCTCCCTCAGCAATGGCGGGGCCATACAAAACCAAGAGAGTGGC ATCAAAGAGTCTGTCTCCTGGGGCACTCAAAGGACCCAGGATGCACCCCATGAGGCCAGGCCACCCCTG GAGCGTGGCCACTCCACAGAGATCCCTGTGGGGCGGTCAGGAGCACTGGCTGTGGACGTTCAGATGCAG AGCATAACCCGGAGGGGCCCTGATGGGGAGCCCCAGCCTGGGCTGGAGAGCCAGGGTCGAGCGGCCTCC ATGCCCCGCCTTGCGGCCGAGACTCAGCCCGTCACAGATGCCAGCCCCATGAAGCGCTCCATCTCCACG CTGGCCCAGCGGCCCCGTGGGACTCATCTTTGCAGCACCACCCCGGACCGCCCACCCCCTAGCCAGGCG TCGTCGCACCACCACCACCACCGCTGCCACCGCCGCAGGGACAGGAAGCAGAGGTCCCTGGAGAAGGGG CCCAGCCTGTCTGCCGATATGGATGGCGCACCAAGCAGTGCTGTGGGGCCGGGGCTGCCCCCGGGAGAG GGGCCTACAGGCTGCCGGCGGGAACGAGAGCGCCGGCAGGAGCGGGGCCGGTCCCAGGAGCGGAGGCAG CCCTCATCCTCCTCCTCGGAGAAGCAGCGCTTCTACTCCTGCGACCGCTTTGGGGGCCGTGAGCCCCCG AAGCCCAAGCCCTCCCTCAGCAGCCACCCAACGTCGCCAACAGCTGGCCAGGAGCCGGGACCCCACCCA CAGGGCAGTGGTTCCGTGAATGGGAGCCCCTTGCTGTCAACATCTGGTGCTAGCACCCCCGGCCGCGGT GGGCGGAGGCAGCTCCCCCAGACGCCCCTGACTCCCCGCCCCAGCATCACCTACAAGACGGCCAACTCC TCACCCATCCACTTCGCCGGGGCTCAGACCAGCCTCCCTGCCTTCTCCCCAGGCCGGCTCAGCCGTGGG CTTTCCGAACACAACGCCCTGCTGCAGAGAGACCCCCTCAGCCAGCCCCTGGCCCCTGGCTCTCGAATT GGCTCTGACCCTTACCTGGGGCAGCGTCTGGACAGTGAGGCCTCTGTCCACGCCCTGCCTGAGGACACT CTCACTTTCGAGGAGGCTGTGGCCACCAACTCGGGCCGCTCCTCCAGGACTTCCTACGTGTCCTCCCTG ACCTCCCAGTCTCACCCTCTCCGCCGCGTGCCCAACGGTTACCACTGCACCCTGGGACTCAGCTCGGGT GGCCGAGCACGGCACAGCTACCACCACCCTGACCAAGACCACTGGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC217170 Blue=ORF Red=Cloning site Green=Tag(s) MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCF TVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFI GIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIP SLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARL CEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSF FMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLD VLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQS FYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFG VIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIV VFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFI VLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQ NSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARG PVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAA GPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGP REAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTV TVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQ AEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPV RTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGK DINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYC TDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYE EQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPL TRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMEC VLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRI LLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEA WHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHH LDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTST LMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDF YKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESG IKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAAS MPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKG PSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPP KPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANS SPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDT LTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000718
ORF Size	7017 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000718.4
RefSeq Size	9805 bp
RefSeq ORF	7020 bp
Locus ID	774
UniProt ID	Q00975
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Calcium signaling pathway, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
MW	262.5 kDa
Gene Summary	The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.