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ZNF207 (NM_001098507) Human Tagged ORF Clone
CAT＃: RC217111
- TrueORF®
ZNF207 (Myc-DDK-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 3
ORF Plasmid: tGFP
"NM_001098507" in other vectors (6)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BuGZ; hBuGZ |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217111 representing NM_001098507
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGCAAGAAGAAGAAGCAGCTGAAGCCGTGGTGCTGGTATTGTAATAGAGATTTTGATGATGAGA AGATCCTTATTCAGCACCAAAAAGCAAAGCATTTTAAATGCCATATATGTCACAAGAAATTGTATACAGG ACCTGGCTTAGCTATTCATTGCATGCAGGTACATAAAGAAACAATAGATGCCGTACCAAATGCAATACCT GGAAGAACAGACATAGAGTTGGAAATATATGGTATGGAAGGTATTCCAGAAAAAGACATGGATGAAAGAC GACGACTTCTTGAACAGAAAACACAAGAAAGTCAAAAAAAGAAGCAACAAGATGATTCTGATGAATATGA TGATGACGACTCTGCAGCCTCAACTTCATTTCAGCCACAGCCTGTTCAACCTCAGCAAGGTTATATTCCT CCAATGGCACAGCCAGGACTGCCACCAGTACCAGGAGCACCAGGAATGCCTCCAGGCATACCTCCATTAA TGCCAGGTGTTCCTCCTCTGATGCCAGGAATGCCACCAGTTATGCCAGGCATGCCACCTGGATTGCATCA TCAGAGAAAATACACCCAGTCATTTTGCGGTGAAAACATAATGATGCCAATGGGTGGAATGATGCCACCT GGACCAGGAATACCACCTCTGATGCCTGGAATGCCACCAGGTATGCCCCCACCTGTTCCACGTCCTGGAA TTCCTCCAATGACTCAAGCACAGGCTGTTTCAGCGCCAGGTATTCTTAATAGACCACCTGCACCAACAGC AACTGTACCTGCCCCACAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAGATGGGGACACCT GTCACAAGCTCAAGTACAGCTTCATCCAATTCAGAAAGTCTGTCTGCATCTTCTAAAGCTCTGTTTCCTA GCACAGCACAAGCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTCAAACCCTTAAATAGTACCCC TGCAACAACTACAGAACCCCCAAAGCCTACATTCCCTGCTTATACACAGTCTACAGCTTCAACAACTAGT ACAACAAATAGTACTGCAGCTAAACCAGCGGCTTCAATAACAAGTAAGCCTGCTACACTTACAACAACTA GTGCAACCAGTAAGTTGATCCATCCAGATGAGGATATATCCCTGGAAGAGAGAAGGGCACAGTTACCTAA GTATCAACGTAATCTTCCTCGGCCAGGACAGGCCCCCATCGGTAATCCACCAGTTGGACCAATTGGAGGT ATGATGCCACCACAGCCAGGCATCCCACAGCAACAAGGAATGAGACCCCCAATGCCACCTCATGGTCAGT ATGGTGGTCATCATCAAGGCATGCCAGGATACCTTCCTGGTGCTATGCCCCCGTATGGGCAGGGACCGCC AATGGTGCCCCCTTACCAGGGTGGGCCTCCTCGACCTCCGATGGGAATGAGACCTCCTGTAATGTCGCAA GGTGGCCGTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217111 representing NM_001098507
Red=Cloning site Green=Tags(s) MGRKKKKQLKPWCWYCNRDFDDEKILIQHQKAKHFKCHICHKKLYTGPGLAIHCMQVHKETIDAVPNAIP GRTDIELEIYGMEGIPEKDMDERRRLLEQKTQESQKKKQQDDSDEYDDDDSAASTSFQPQPVQPQQGYIP PMAQPGLPPVPGAPGMPPGIPPLMPGVPPLMPGMPPVMPGMPPGLHHQRKYTQSFCGENIMMPMGGMMPP GPGIPPLMPGMPPGMPPPVPRPGIPPMTQAQAVSAPGILNRPPAPTATVPAPQPPVTKPLFPSAGQMGTP VTSSSTASSNSESLSASSKALFPSTAQAQAAVQGPVGTDFKPLNSTPATTTEPPKPTFPAYTQSTASTTS TTNSTAAKPAASITSKPATLTTTSATSKLIHPDEDISLEERRAQLPKYQRNLPRPGQAPIGNPPVGPIGG MMPPQPGIPQQQGMRPPMPPHGQYGGHHQGMPGYLPGAMPPYGQGPPMVPPYQGGPPRPPMGMRPPVMSQ GGRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098507 |
| ORF Size | 1482 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001098507.2 |
| RefSeq Size | 2333 bp |
| RefSeq ORF | 1485 bp |
| Locus ID | 7756 |
| UniProt ID | O43670 |
| Protein Families | Transcription Factors |
| MW | 52.5 kDa |
| Gene Summary | Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217111L1 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 3, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC217111L2 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 3, mGFP tagged |
CNY 6,180.00 |
|
| RC217111L3 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 3, Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC217111L4 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 3, mGFP tagged |
CNY 6,180.00 |
|
| RG217111 | ZNF207 (tGFP-tagged) - Human zinc finger protein 207 (ZNF207), transcript variant 3 |
CNY 4,750.00 |
|
| SC316390 | ZNF207 (untagged)-Human zinc finger protein 207 (ZNF207), transcript variant 3 |
CNY 7,890.00 |
