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PAPSS1 (NM_005443) Human Tagged ORF Clone
CAT＃: RC217023
PAPSS1 (Myc-DDK-tagged)-Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1)
ORF Plasmid: tGFP
"NM_005443" in other vectors (4)
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CNY 6,968.00
CNY 7,315.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATPSK1; PAPSS; SK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217023 representing NM_005443
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATCCCCGGGAGCCTGTGCAAGAAAGTCAAACTGAGCAATAACGCGCAGAACTGGGGAATGCAGA GAGCAACCAATGTCACCTACCAAGCCCATCATGTCAGCAGGAACAAGAGAGGTCAGGTGGTGGGGACCAG AGGTGGCTTTCGTGGTTGCACAGTTTGGCTAACAGGCTTGTCTGGAGCGGGAAAGACTACTGTGAGCATG GCCTTGGAGGAGTACCTGGTTTGTCATGGTATTCCATGCTACACTCTGGATGGTGACAATATTCGTCAAG GTCTCAATAAAAATCTTGGCTTTAGTCCTGAAGACAGAGAAGAGAATGTTCGACGCATCGCAGAAGTTGC TAAACTGTTTGCAGATGCTGGCTTAGTGTGCATCACAAGTTTCATATCACCTTACACTCAGGATCGCAAC AATGCAAGGCAAATTCATGAAGGTGCAAGTTTACCGTTTTTTGAAGTATTTGTTGATGCTCCTCTGCATG TTTGTGAACAGAGGGATGTCAAAGGACTCTACAAAAAAGCCCGGGCAGGAGAAATTAAAGGTTTCACTGG GATCGATTCTGAATATGAAAAGCCAGAGGCCCCTGAGTTGGTGCTGAAAACAGACTCCTGTGATGTAAAT GACTGTGTCCAGCAAGTTGTGGAACTTCTACAGGAACGGGATATTGTACCTGTGGATGCATCTTATGAAG TAAAAGAACTATATGTGCCAGAAAATAAACTTCATTTGGCAAAAACAGATGCGGAAACATTACCAGCACT GAAAATTAATAAAGTGGATATGCAGTGGGTGCAGGTTTTGGCAGAAGGTTGGGCAACCCCATTGAATGGC TTTATGAGAGAGAGGGAGTACTTGCAGTGCCTTCATTTTGATTGTCTTCTGGATGGAGGTGTCATTAACT TGTCAGTACCTATAGTTCTGACTGCGACTCATGAAGATAAAGAGAGGCTGGACGGCTGTACAGCATTTGC TCTGATGTATGAGGGCCGCCGTGTGGCCATTCTTCGCAATCCAGAGTTTTTTGAGCACAGGAAAGAGGAG CGCTGTGCCAGACAGTGGGGAACGACATGCAAGAACCACCCCTATATTAAGATGGTGATGGAACAAGGAG ATTGGCTGATTGGAGGAGATCTTCAAGTCTTGGATCGAGTTTATTGGAATGATGGTCTTGATCAGTATCG TCTTACTCCTACTGAGCTAAAGCAGAAATTTAAAGATATGAATGCTGATGCTGTCTTTGCATTTCAACTA CGCAACCCAGTGCACAATGGACATGCCCTGTTAATGCAGGATACCCATAAGCAACTTCTAGAGAGGGGCT ACCGGCGCCCTGTCCTCCTCCTCCACCCTCTGGGTGGCTGGACAAAGGATGACGATGTTCCTTTGATGTG GCGTATGAAGCAGCATGCTGCAGTGTTGGAGGAAGGAGTTCTGAATCCTGAGACGACAGTGGTGGCCATC TTCCCATCTCCCATGATGTATGCTGGACCAACTGAGGTCCAGTGGCATTGCAGAGCACGGATGGTTGCAG GAGCCAACTTTTACATTGTTGGACGAGACCCTGCTGGCATGCCTCATCCAGAAACAGGGAAGGATCTTTA TGAGCCAAGTCATGGTGCCAAAGTGCTGACGATGGCCCCTGGTTTAATCACTTTGGAAATAGTTCCCTTT CGAGTTGCAGCTTACAACAAGAAAAAGAAGCGTATGGACTACTATGACTCTGAACACCATGAAGACTTTG AATTTATTTCAGGAACACGAATGCGCAAACTTGCTCGAGAAGGCCAGAAACCACCTGAAGGTTTCATGGC TCCCAAGGCTTGGACCGTGCTGACAGAATACTACAAATCCTTGGAGAAAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217023 representing NM_005443
Red=Cloning site Green=Tags(s) MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSM ALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRN NARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVN DCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNG FMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEE RCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQL RNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAI FPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPF RVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005443 |
| ORF Size | 1872 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005443.5 |
| RefSeq Size | 2558 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 9061 |
| UniProt ID | O43252 |
| Domains | ATP-sulfurylase, APS_kinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Purine metabolism, Selenoamino acid metabolism, Sulfur metabolism |
| MW | 70.7 kDa |
| Gene Summary | Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217023L3 | Lenti ORF clone of Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217023L4 | Lenti ORF clone of Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mGFP tagged |
CNY 5,890.00 |
|
| RG217023 | PAPSS1 (tGFP-tagged) - Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1) |
CNY 8,568.00 |
|
| SC314249 | PAPSS1 (untagged)-Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1) |
CNY 6,976.00 |
