Deoxyribonuclease I like 1 (DNASE1L1) (NM_001009932) Human Tagged ORF Clone
CAT#: RC216956
DNASE1L1 (Myc-DDK-tagged)-Human deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 2
ORF Plasmid: tGFP
"NM_001009932" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DNAS1L1; DNASEX; DNL1L; G4.8; XIB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216956 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTACCCAACTGCACTCCTCTTCCTCATCCTGGCCAATGGGGCCCAGGCCTTTCGCATCTGCGCCT TCAATGCCCAGCGGCTGACACTGGCCAAGGTGGCCAGGGAGCAGGTGATGGACACCTTAGTTCGGATACT GGCTCGCTGTGACATCATGGTGCTGCAGGAGGTGGTGGACTCTTCCGGCAGCGCCATCCCCCTCCTGCTT CGAGAACTCAATCGATTTGATGGCTCTGGGCCCTACAGCACCCTGAGCAGCCCCCAGCTGGGGCGCAGCA CCTACATGGAGACGTATGTGTACTTCTATCGGTCACACAAAACACAGGTCCTGAGTTCCTACGTGTACAA CGATGAGGATGACGTCTTTGCCCGGGAGCCATTTGTGGCCCAGTTCTCTTTGCCCAGCAATGTCCTTCCC AGCCTGGTGTTGGTCCCGCTGCACACCACTCCTAAGGCCGTAGAGAAGGAGCTGAACGCCCTCTACGATG TGTTTCTGGAGGTCTCCCAGCACTGGCAGAGCAAGGACGTGATCCTGCTTGGGGACTTCAATGCTGACTG CGCTTCACTGACCAAAAAGCGCCTGGACAAGCTGGAGCTGCGGACTGAGCCAGGCTTCCACTGGGTGATT GCCGATGGGGAGGACACCACAGTGCGGGCCAGCACCCACTGCACCTATGACCGCGTCGTGCTGCACGGGG AGCGCTGCCGGAGTCTGCTGCACACTGCGGCTGCCTTTGACTTCCCCACGAGCTTCCAGCTCACCGAGGA GGAGGCCCTCAACATCAGTGACCACTACCCCGTGGAGGTGGAGCTGAAGCTGAGCCAGGCACACAGCGTC CAGCCTCTCAGCCTCACTGTTCTGTTGCTGCTATCACTCCTGTCCCCTCAGCTGTGCCCTGCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216956 protein sequence
Red=Cloning site Green=Tags(s) MHYPTALLFLILANGAQAFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLL RELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLP SLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVI ADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSV QPLSLTVLLLLSLLSPQLCPAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001009932 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001009932.2, NP_001009932.1 |
RefSeq Size | 3008 bp |
RefSeq ORF | 909 bp |
Locus ID | 1774 |
UniProt ID | P49184 |
MW | 33.9 kDa |
Gene Summary | This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed. [provided by RefSeq, Jan 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216956L3 | Lenti ORF clone of Human deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216956L4 | Lenti ORF clone of Human deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG216956 | DNASE1L1 (tGFP-tagged) - Human deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 2 |
CNY 4,000.00 |
|
SC301446 | DNASE1L1 (untagged)-Human deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 2 |
CNY 6,270.00 |
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