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KIF5B (NM_004521) Human Tagged ORF Clone
CAT＃: RC216778
- TrueORF®
KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B)
ORF Plasmid: tGFP
"NM_004521" in other vectors (6)
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CNY 9,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-61; KINH; KNS; KNS1; UKHC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216778 representing NM_004521
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCTGGCCGAGTGCAACATCAAAGTGATGTGTCGCTTCAGACCTCTCAACGAGTCTGAAGTGA ACCGCGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCGCGTCCAAGCCTTATGC ATTTGATCGGGTGTTCCAGTCAAGCACATCTCAAGAGCAAGTGTATAATGACTGTGCAAAGAAGATTGTT AAAGATGTACTTGAAGGATATAATGGAACAATATTTGCATATGGACAAACATCCTCTGGGAAGACACACA CAATGGAGGGTAAACTTCATGATCCAGAAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAA TTATATTTACTCCATGGATGAAAATTTGGAATTTCATATTAAGGTTTCATATTTTGAAATATATTTGGAT AAGATAAGGGACCTGTTAGATGTTTCAAAGACCAACCTTTCAGTTCATGAAGACAAAAACCGAGTTCCCT ATGTAAAGGGGTGCACAGAGCGTTTTGTATGTAGTCCAGATGAAGTTATGGATACCATAGATGAAGGAAA ATCCAACAGACATGTAGCAGTTACAAATATGAATGAACATAGCTCTAGGAGTCACAGTATATTTCTTATT AATGTCAAACAAGAGAACACACAAACGGAACAAAAGCTGAGTGGAAAACTTTATCTGGTTGATTTAGCTG GTAGTGAAAAGGTTAGTAAAACTGGAGCTGAAGGTGCTGTGCTGGATGAAGCTAAAAACATCAACAAGTC ACTTTCTGCTCTTGGAAATGTTATTTCTGCTTTGGCTGAGGGTAGTACATATGTTCCATATCGAGATAGT AAAATGACAAGAATCCTTCAAGATTCATTAGGTGGCAACTGTAGAACCACTATTGTAATTTGCTGCTCTC CATCATCATACAATGAGTCTGAAACAAAATCTACACTCTTATTTGGCCAAAGGGCCAAAACAATTAAGAA CACAGTTTGTGTCAATGTGGAGTTAACTGCAGAACAGTGGAAAAAGAAGTATGAAAAAGAAAAAGAAAAA AATAAGATCCTGCGGAACACTATTCAGTGGCTTGAAAATGAGCTCAACAGATGGCGTAATGGGGAGACGG TGCCTATTGATGAACAGTTTGACAAAGAGAAAGCCAACTTGGAAGCTTTCACAGTGGATAAAGATATTAC TCTTACCAATGATAAACCAGCAACCGCAATTGGAGTTATAGGAAATTTTACTGATGCTGAAAGAAGAAAG TGTGAAGAAGAAATTGCTAAATTATACAAACAGCTTGATGACAAGGATGAAGAAATTAACCAGCAAAGTC AACTGGTAGAGAAACTGAAGACGCAAATGTTGGATCAGGAGGAGCTTTTGGCATCTACCAGAAGGGATCA AGACAATATGCAAGCTGAGCTGAATCGCCTTCAAGCAGAAAATGATGCCTCTAAAGAAGAAGTGAAAGAA GTTTTACAGGCCCTAGAAGAACTTGCTGTCAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACTA AGGAATATGAATTGCTTAGTGATGAATTGAATCAGAAATCGGCAACTTTAGCGAGTATAGATGCTGAGCT TCAGAAACTTAAGGAAATGACCAACCACCAGAAAAAACGAGCAGCTGAGATGATGGCATCTTTACTAAAA GACCTTGCAGAAATAGGAATTGCTGTGGGAAATAATGATGTAAAGCAGCCTGAGGGAACTGGCATGATAG ATGAAGAGTTCACTGTTGCAAGACTCTACATTAGCAAAATGAAGTCAGAAGTAAAAACCATGGTGAAACG TTGCAAGCAGTTAGAAAGCACACAAACTGAGAGCAACAAAAAAATGGAAGAAAATGAAAAGGAGTTAGCA GCATGTCAGCTTCGTATCTCTCAACATGAAGCCAAAATCAAGTCATTGACTGAATACCTTCAAAATGTGG AACAAAAGAAAAGACAGTTGGAGGAATCTGTCGATGCCCTCAGTGAAGAACTAGTCCAGCTTCGAGCACA AGAGAAAGTCCATGAAATGGAAAAGGAGCACTTAAATAAGGTTCAGACTGCAAATGAAGTTAAGCAAGCT GTTGAACAGCAGATCCAGAGCCATAGAGAAACTCATCAAAAACAGATCAGTAGTTTGAGAGATGAAGTAG AAGCAAAAGCAAAACTTATTACTGATCTTCAAGACCAAAACCAGAAAATGATGTTAGAGCAGGAACGTCT AAGAGTAGAACATGAGAAGTTGAAAGCCACAGATCAGGAAAAGAGCAGAAAACTACATGAACTTACGGTT ATGCAAGATAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAAGAGACAGTGGCAAAAGAACTTC AGACTTTACACAACCTGCGCAAACTCTTTGTTCAGGACCTGGCTACAAGAGTTAAAAAGAGTGCTGAGAT TGATTCTGATGACACCGGAGGCAGCGCTGCTCAGAAGCAAAAAATCTCCTTTCTTGAAAATAATCTTGAA CAGCTCACTAAAGTGCACAAACAGTTGGTACGTGATAATGCAGATCTCCGCTGTGAACTTCCTAAGTTGG AAAAGCGACTTCGAGCTACAGCTGAGAGAGTGAAAGCTTTGGAATCAGCACTGAAAGAAGCTAAAGAAAA TGCATCTCGTGATCGCAAACGCTATCAGCAAGAAGTAGATCGCATAAAGGAAGCAGTCAGGTCAAAGAAT ATGGCCAGAAGAGGGCATTCTGCACAGATTGCTAAACCTATTCGTCCCGGGCAACATCCAGCAGCTTCTC CAACTCACCCAAGTGCAATTCGTGGAGGAGGTGCATTTGTTCAGAACAGCCAGCCAGTGGCAGTGCGAGG TGGAGGAGGCAAACAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216778 representing NM_004521
Red=Cloning site Green=Tags(s) MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIV KDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLD KIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLI NVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDS KMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEK NKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRK CEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKE VLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLK DLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELA ACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQA VEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTV MQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLE QLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKN MARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004521 |
| ORF Size | 2889 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004521.3 |
| RefSeq Size | 3688 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 3799 |
| UniProt ID | P33176 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 109.5 kDa |
| Gene Summary | Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS
,null,
Communications Biology
,PubMed ID 37386082
[KIF5B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216778L1 | Lenti-ORF clone of KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B) |
CNY 11,500.00 |
|
| RC216778L2 | Lenti-ORF clone of KIF5B (mGFP-tagged)-Human kinesin family member 5B (KIF5B) |
CNY 12,976.00 |
|
| RC216778L3 | Lenti-ORF clone of KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B) |
CNY 11,500.00 |
|
| RC216778L4 | Lenti-ORF clone of KIF5B (mGFP-tagged)-Human kinesin family member 5B (KIF5B) |
CNY 11,500.00 |
|
| RG216778 | KIF5B (tGFP-tagged) - Human kinesin family member 5B (KIF5B) |
CNY 12,176.00 |
|
| SC117316 | KIF5B (untagged)-Human kinesin family member 5B (KIF5B) |
CNY 10,592.00 |
