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CHD3 (NM_001005273) Human Tagged ORF Clone
CAT＃: RC216755
CHD3 (Myc-DDK-tagged)-Human chromodomain helicase DNA binding protein 3 (CHD3), transcript variant 1
ORF Plasmid: tGFP
"NM_001005273" in other vectors (3)
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CNY 13,440.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216755 representing NM_001005273
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCGGCAGACACTGTGATCCTGTGGGCAAGAAGTAAAAATGACCAGCTGAGGATTTCTTTTCCTC CAGGACTGTGTTGGGGTGACAGGATGCCTGATAAGGATGACATTCGGCTGCTGCCGTCAGCATTGGGTGT GAAGAAGAGAAAACGAGGACCCAAGAAGCAGAAGGAGAACAAGCCAGGAAAACCCCGAAAACGCAAGAAG CGTGACAGTGAGGAGGAATTTGGTTCTGAGCGAGATGAGTACCGGGAGAAGTCAGAGAGTGGGGGCAGTG AATATGGAACCGGACCGGGTCGGAAACGAAGAAGGAAGCACCGAGAAAAAAAGGAGAAGAAGACAAAGCG GCGGAAAAAGGGGGAGGGAGATGGGGGGCAAAAGCAAGTGGAACAGAAGTCATCAGCAACTCTGCTTCTG ACCTGGGGCCTGGAGGATGTGGAGCATGTGTTCTCTGAGGAGGATTACCACACGCTCACCAACTACAAAG CCTTCAGCCAGTTCATGAGGCCCCTAATTGCTAAGAAGAATCCTAAGATCCCAATGTCTAAGATGATGAC CATCCTTGGGGCCAAATGGAGAGAGTTCAGTGCCAACAACCCCTTCAAGGGGTCAGCAGCTGCTGTGGCG GCGGCAGCGGCAGCAGCAGCAGCAGCTGTAGCTGAGCAGGTGTCAGCTGCTGTCTCGTCGGCCACCCCCA TAGCACCCTCCGGACCCCCCGCCCTTCCACCACCCCCTGCTGCTGATATCCAGCCCCCACCCATCCGAAG AGCCAAAACCAAAGAGGGCAAAGGTCCAGGCCATAAGAGGCGGAGTAAGAGCCCCCGAGTGCCTGATGGA CGCAAGAAGCTTCGGGGAAAGAAAATGGCACCACTCAAAATAAAACTAGGGCTTCTGGGTGGCAAGAGGA AGAAAGGAGGCTCGTATGTTTTTCAGAGCGACGAAGGTCCTGAACCAGAGGCTGAGGAATCAGACCTGGA CAGTGGCAGTGTCCACAGTGCCTCAGGCCGGCCTGATGGCCCTGTCCGCACCAAGAAACTAAAGAGAGGC CGGCCAGGAAGGAAGAAGAAGAAGGTCCTGGGCTGTCCTGCAGTGGCCGGGGAGGAGGAGGTTGATGGCT ACGAGACGGATCACCAGGATTACTGTGAGGTGTGCCAGCAGGGTGGGGAAATTATTCTGTGTGACACCTG CCCTCGTGCCTACCACCTCGTCTGCCTTGATCCTGAGCTTGACCGGGCTCCAGAGGGCAAATGGAGCTGC CCTCACTGTGAGAAGGAGGGGGTCCAGTGGGAGGCCAAGGAGGAAGAAGAAGAATACGAAGAGGAGGGAG AGGAAGAAGGGGAGAAGGAGGAGGAGGATGATCACATGGAGTACTGCCGCGTATGCAAGGACGGCGGGGA GCTCCTGTGCTGTGACGCGTGCATCTCCTCCTACCACATTCATTGTCTAAACCCTCCCCTGCCTGACATT CCCAATGGTGAATGGCTGTGTCCCCGATGCACATGCCCCGTGCTGAAGGGTCGAGTGCAGAAGATCCTAC ATTGGCGGTGGGGGGAGCCACCTGTAGCAGTGCCAGCCCCTCAACAGGCAGATGGAAATCCAGATGTCCC ACCCCCCCGTCCTCTTCAAGGCAGATCAGAGCGAGAGTTCTTTGTCAAGTGGGTAGGACTATCCTACTGG CACTGCTCCTGGGCCAAGGAGCTTCAGCTGGAAATCTTCCATTTGGTTATGTATCGAAACTACCAGCGGA AGAATGACATGGATGAGCCCCCACCCCTGGACTATGGCTCCGGCGAGGATGATGGGAAGAGCGACAAGCG TAAAGTGAAAGACCCGCACTATGCTGAGATGGAGGAGAAGTACTATCGTTTTGGCATCAAGCCAGAGTGG ATGACCGTCCACCGCATCATCAACCACAGTGTGGATAAAAAGGGGAATTACCACTATCTAGTAAAATGGA GGGACTTACCATATGACCAGTCCACGTGGGAGGAAGATGAAATGAATATCCCTGAATACGAAGAACATAA GCAAAGCTACTGGAGACACCGAGAACTAATTATGGGGGAAGACCCTGCCCAGCCCCGCAAGTATAAGAAG AAGAAGAAGGAGCTACAGGGTGATGGGCCTCCCAGTTCTCCCACTAATGATCCTACCGTGAAATATGAGA CTCAGCCACGGTTTATCACAGCCACTGGAGGCACCCTGCACATGTATCAGTTGGAAGGGCTGAACTGGCT ACGCTTCTCCTGGGCCCAGGGCACTGACACCATTCTAGCTGATGAGATGGGGCTAGGCAAGACCATACAA ACCATCGTCTTCCTCTACTCACTCTACAAGGAGGGCCACACAAAAGGTCCCTTCCTGGTGAGTGCCCCAC TCTCTACCATCATTAACTGGGAGCGGGAGTTCCAGATGTGGGCACCCAAATTCTATGTGGTGACATACAC GGGTGACAAGGACAGCCGGGCCATCATTCGTGAGAATGAATTCTCCTTTGAGGACAATGCCATCAAAGGG GGCAAGAAAGCTTTTAAGATGAAGAGGGAGGCACAGGTGAAGTTCCATGTTCTCCTGACATCGTATGAGC TGATCACCATTGATCAGGCAGCACTTGGTTCCATCCGCTGGGCCTGTCTTGTGGTAGATGAGGCCCATCG ACTCAAGAACAACCAGTCCAAGTTTTTCAGGGTTCTCAATGGTTACAAGATAGATCATAAGTTGCTGCTG ACAGGAACCCCATTGCAGAATAATCTGGAGGAGCTCTTCCATCTCCTGAACTTCCTCACCCCAGAGAGAT TTAACAACTTGGAGGGCTTCCTGGAGGAGTTTGCTGACATATCCAAAGAGGACCAGATCAAGAAACTGCA TGATTTGCTGGGGCCACACATGCTGCGGAGACTCAAGGCAGATGTCTTTAAGAACATGCCAGCCAAGACA GAGCTCATCGTTCGGGTGGAGCTAAGCCCCATGCAGAAGAAATACTACAAATACATCCTGACTCGAAATT TTGAGGCCTTGAATTCACGAGGTGGTGGGAACCAGGTGTCGCTGCTTAATATCATGATGGATCTTAAGAA GTGCTGCAACCATCCATACCTTTTTCCCGTGGCTGCTATGGAGTCCCCCAAACTCCCCAGTGGGGCTTAT GAGGGTGGGGCACTTATTAAGTCGTCTGGGAAGCTCATGCTGCTCCAGAAGATGCTGCGAAAGCTGAAGG AGCAAGGACACCGAGTGCTCATCTTCTCGCAGATGACCAAAATGTTAGACTTGCTTGAGGACTTCTTAGA CTATGAAGGCTACAAGTATGAGCGCATCGATGGTGGTATCACGGGTGCCCTGAGGCAGGAGGCCATCGAT CGGTTTAATGCTCCTGGGGCCCAACAATTCTGCTTCCTCCTGTCCACCCGAGCTGGGGGCCTGGGCATCA ATCTGGCCACTGCTGACACTGTCATCATCTTTGATTCTGACTGGAACCCCCATAATGACATCCAGGCCTT TAGCCGGGCTCATCGGATTGGCCAGGCCAACAAAGTGATGATTTACCGGTTTGTGACTCGCGCGTCAGTG GAAGAGCGAATCACACAAGTGGCCAAGAGAAAGATGATGCTGACACACCTGGTTGTGCGGCCTGGGCTGG GCTCCAAGGCAGGCTCCATGTCCAAGCAGGAGCTTGACGACATTCTCAAATTTGGCACTGAAGAGCTATT CAAGGATGAAAACGAGGGGGAGAACAAGGAGGAGGACAGCAGTGTGATTCATTATGACAATGAGGCCATC GCTCGGCTGTTGGACCGGAACCAGGATGCAACTGAGGACACTGACGTGCAGAACATGAATGAGTATCTCA GCTCCTTCAAGGTGGCACAGTACGTCGTGCGGGAAGAAGACAAGATTGAGGAAATTGAGCGAGAGATCAT CAAGCAGGAGGAGAATGTGGACCCTGACTACTGGGAGAAGCTGCTGAGGCATCACTATGAGCAACAGCAG GAAGACCTAGCCCGGAATCTAGGCAAGGGCAAGCGGGTTCGCAAGCAAGTTAACTACAATGATGCTGCTC AGGAAGACCAAGACAACCAGTCAGAGTACTCGGTGGGTTCAGAGGAGGAGGATGAAGACTTCGATGAACG TCCTGAAGGGCGTAGACAGTCAAAGAGGCAGCTCCGGAATGAGAAAGATAAGCCACTGCCTCCACTGCTG GCCCGAGTCGGGGGCAACATTGAGGTGCTGGGCTTCAACACCCGTCAGCGGAAGGCTTTCCTCAATGCTG TGATGCGCTGGGGGATGCCACCACAGGATGCCTTCACCACACAGTGGCTGGTGCGGGACCTGAGGGGCAA GACTGAGAAGGAGTTTAAGGCCTATGTGTCTTTGTTCATGCGCCATCTGTGTGAGCCTGGGGCAGACGGC TCTGAAACCTTTGCCGATGGGGTCCCTCGGGAGGGACTGAGTCGCCAGCAGGTGTTGACCCGCATTGGAG TCATGTCTCTCGTCAAAAAGAAGGTGCAGGAGTTTGAGCACATCAATGGGCGTTGGTCAATGCCGGAACT GATGCCTGACCCCAGCGCCGATTCTAAGCGCTCCTCCAGAGCCTCCTCTCCTACCAAAACGTCTCCCACC ACTCCTGAGGCTTCTGCTACCAACAGTCCCTGCACCTCTAAACCTGCTACTCCAGCTCCAAGTGAGAAAG GAGAAGGCATAAGGACACCTCTTGAGAAGGAGGAAGCTGAAAACCAGGAGGAAAAGCCAGAGAAGAACAG CAGAATTGGGGAGAAGATGGAGACAGAGGCTGATGCCCCCAGCCCAGCCCCATCACTTGGGGAGCGGCTG GAGCCAAGGAAGATTCCTCTAGAGGATGAGGTGCCAGGGGTGCCTGGAGAGATGGAGCCTGAACCTGGGT ACCGTGGGGACAGAGAGAAGTCAGCCACAGAGTCGACGCCAGGAGAAAGGGGGGAGGAGAAGCCGTTGGA TGGACAGGAACACAGGGAGAGGCCGGAGGGGGAAACAGGGGATTTGGGCAAGAGAGAAGATGTAAAAGGT GACCGGGAGCTTCGACCAGGGCCTCGAGATGAGCCACGGTCCAATGGGCGACGAGAGGAAAAGACAGAGA AGCCCCGGTTCATGTTCAATATCGCCGATGGTGGCTTCACAGAGCTTCACACACTGTGGCAGAATGAGGA ACGGGCAGCTATTTCCTCGGGGAAACTCAATGAGATCTGGCACAGAAGACATGACTATTGGCTTCTGGCT GGGATTGTCCTCCATGGCTATGCACGGTGGCAGGACATCCAGAATGATGCTCAATTTGCCATTATCAACG AGCCATTTAAAACTGAAGCCAATAAGGGGAACTTTCTGGAGATGAAAAATAAGTTCCTGGCCCGGAGGTT CAAGCTCCTGGAGCAGGCGCTGGTGATTGAGGAGCAGCTGCGGCGGGCGGCCTACCTGAACCTGTCGCAG GAGCCGGCGCACCCCGCCATGGCCCTCCACGCCCGCTTCGCCGAGGCCGAGTGCCTGGCCGAGAGCCACC AGCACCTCTCCAAGGAGTCGCTGGCGGGGAACAAGCCGGCCAACGCCGTCCTGCACAAGGTTCTGAACCA GCTGGAGGAGTTGCTGAGCGACATGAAGGCGGACGTGACCCGCCTGCCAGCCACGCTGTCCCGAATACCC CCCATCGCAGCCCGCCTTCAGATGTCCGAGCGCAGCATCCTCAGCCGGCTGGCCAGCAAGGGCACGGAGC CTCACCCCACACCGGCCTACCCGCCGGGTCCCTACGCTACACCTCCGGGGTACGGGGCGGCCTTCAGCGC CGCACCCGTAGGGGCCCTGGCCGCCGCAGGCGCCAATTACAGCCAGATGCCTGCAGGGTCCTTCATCACA GCCGCCACCAACGGCCCTCCAGTGCTTGTGAAGAAGGAGAAGGAAATGGTGGGGGCATTGGTGTCAGACG GGCTGGATCGGAAGGAGCCCCGAGCCGGGGAGGTGATCTGTATAGACGAC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RC216755 representing NM_001005273
Red=Cloning site Green=Tags(s) MKAADTVILWARSKNDQLRISFPPGLCWGDRMPDKDDIRLLPSALGVKKRKRGPKKQKENKPGKPRKRKK RDSEEEFGSERDEYREKSESGGSEYGTGPGRKRRRKHREKKEKKTKRRKKGEGDGGQKQVEQKSSATLLL TWGLEDVEHVFSEEDYHTLTNYKAFSQFMRPLIAKKNPKIPMSKMMTILGAKWREFSANNPFKGSAAAVA AAAAAAAAAVAEQVSAAVSSATPIAPSGPPALPPPPAADIQPPPIRRAKTKEGKGPGHKRRSKSPRVPDG RKKLRGKKMAPLKIKLGLLGGKRKKGGSYVFQSDEGPEPEAEESDLDSGSVHSASGRPDGPVRTKKLKRG RPGRKKKKVLGCPAVAGEEEVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELDRAPEGKWSC PHCEKEGVQWEAKEEEEEYEEEGEEEGEKEEEDDHMEYCRVCKDGGELLCCDACISSYHIHCLNPPLPDI PNGEWLCPRCTCPVLKGRVQKILHWRWGEPPVAVPAPQQADGNPDVPPPRPLQGRSEREFFVKWVGLSYW HCSWAKELQLEIFHLVMYRNYQRKNDMDEPPPLDYGSGEDDGKSDKRKVKDPHYAEMEEKYYRFGIKPEW MTVHRIINHSVDKKGNYHYLVKWRDLPYDQSTWEEDEMNIPEYEEHKQSYWRHRELIMGEDPAQPRKYKK KKKELQGDGPPSSPTNDPTVKYETQPRFITATGGTLHMYQLEGLNWLRFSWAQGTDTILADEMGLGKTIQ TIVFLYSLYKEGHTKGPFLVSAPLSTIINWEREFQMWAPKFYVVTYTGDKDSRAIIRENEFSFEDNAIKG GKKAFKMKREAQVKFHVLLTSYELITIDQAALGSIRWACLVVDEAHRLKNNQSKFFRVLNGYKIDHKLLL TGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKT ELIVRVELSPMQKKYYKYILTRNFEALNSRGGGNQVSLLNIMMDLKKCCNHPYLFPVAAMESPKLPSGAY EGGALIKSSGKLMLLQKMLRKLKEQGHRVLIFSQMTKMLDLLEDFLDYEGYKYERIDGGITGALRQEAID RFNAPGAQQFCFLLSTRAGGLGINLATADTVIIFDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRASV EERITQVAKRKMMLTHLVVRPGLGSKAGSMSKQELDDILKFGTEELFKDENEGENKEEDSSVIHYDNEAI ARLLDRNQDATEDTDVQNMNEYLSSFKVAQYVVREEDKIEEIEREIIKQEENVDPDYWEKLLRHHYEQQQ EDLARNLGKGKRVRKQVNYNDAAQEDQDNQSEYSVGSEEEDEDFDERPEGRRQSKRQLRNEKDKPLPPLL ARVGGNIEVLGFNTRQRKAFLNAVMRWGMPPQDAFTTQWLVRDLRGKTEKEFKAYVSLFMRHLCEPGADG SETFADGVPREGLSRQQVLTRIGVMSLVKKKVQEFEHINGRWSMPELMPDPSADSKRSSRASSPTKTSPT TPEASATNSPCTSKPATPAPSEKGEGIRTPLEKEEAENQEEKPEKNSRIGEKMETEADAPSPAPSLGERL EPRKIPLEDEVPGVPGEMEPEPGYRGDREKSATESTPGERGEEKPLDGQEHRERPEGETGDLGKREDVKG DRELRPGPRDEPRSNGRREEKTEKPRFMFNIADGGFTELHTLWQNEERAAISSGKLNEIWHRRHDYWLLA GIVLHGYARWQDIQNDAQFAIINEPFKTEANKGNFLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNLSQ EPAHPAMALHARFAEAECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPATLSRIP PIAARLQMSERSILSRLASKGTEPHPTPAYPPGPYATPPGYGAAFSAAPVGALAAAGANYSQMPAGSFIT AATNGPPVLVKKEKEMVGALVSDGLDRKEPRAGEVICIDD TRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005273 |
| ORF Size | 6000 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005273.3 |
| RefSeq Size | 6831 bp |
| RefSeq ORF | 6003 bp |
| Locus ID | 1107 |
| UniProt ID | Q12873 |
| Protein Families | Druggable Genome |
| MW | 226.4 kDa |
| Gene Summary | This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216755L1 | Lenti ORF clone of Human chromodomain helicase DNA binding protein 3 (CHD3), transcript variant 1, Myc-DDK-tagged |
CNY 15,840.00 |
|
| RG216755 | CHD3 (tGFP-tagged) - Human chromodomain helicase DNA binding protein 3 (CHD3), transcript variant 1 |
CNY 15,040.00 |
|
| SC300878 | CHD3 (untagged)-Human chromodomain helicase DNA binding protein 3 (CHD3), transcript variant 1 |
CNY 13,208.00 |
