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ARHGAP10 (NM_024605) Human Tagged ORF Clone
CAT＃: RC216597
ARHGAP10 (Myc-DDK-tagged)-Human Rho GTPase activating protein 10 (ARHGAP10)
ORF Plasmid: tGFP
"NM_024605" in other vectors (7)
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CNY 8,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GRAF2; PS-GAP; PSGAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216597 representing NM_024605
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCAGCCCCTGGAGTTCAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAGAGGATCCGCG CTCACGAAGCGGAACTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATTAAGGACGGGAAGAACCTCAT CGCTGCGACGAAAAGTCTGTCAGTGGCCCAGCGGAAGTTTGCTCATTCACTCAGAGACTTTAAGTTTGAG TTTATCGGTGATGCTGTGACAGATGATGAACGATGCATAGATGCTTCCTTACGTGAATTTTCAAATTTTT TGAAGAATCTGGAGGAACAGAGAGAAATTATGGCATTAAGTGTAACTGAAACCCTGATTAAACCCTTGGA AAAATTCAGAAAAGAGCAACTTGGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACAGAAAAG AATTATAGTCTAATTGATAAACATTTGAATTTATCAGCAAAAAAGAAAGACTCACATTTACAAGAGGCAG ATATCCAAGTAGAGCAGAACCGGCAACACTTCTATGAACTGTCTCTCGAGTATGTGTGTAAGCTGCAGGA AATCCAAGAAAGAAAGAAGTTTGAGTTTGTGGAACCTATGCTGTCATTTTTTCAGGGGATGTTTACCTTC TATCATCAGGGCCATGAACTTGCCAAAGACTTCAATCACTACAAAATGGAACTACAGATCAACATTCAGA ATACACGGAATCGATTTGAAGGAACAAGGTCAGAAGTGGAAGAGCTCATGAACAAAATCAGACAGAATCC CAAGGACCACAAACGAGCAAGTCAGTTTACAGCCGAAGGCTACCTGTATGTCCAGGAAAAAAGGCCTGCT CCGTTTGGTTCCAGTTGGGTCAAACACTATTGCATGTATCGAAAAGCAGCAAAGAAGTTCAACATGATCC CATTTGAGCACAGATCTGGAGGGAAACTTGGGGACGGAGAGGTGTTCTTTTTGAAAGAATGTACCAAGAG GCATACTGACTCCATTGACAGAAGGTTTTGTTTTGACATAGAAGCTGCTGATCGGCCTGGCGTTTCCTTG ACCATGCAGGCATTTTCCGAAGAGGAAAGGAAGCAGTGGTTGGAAGCTCTGGGTGGAAAGGAAGCTCTGT CCCATAGTTTTAATACAGCCATCATCCCAAGACCAGAAGGAAATGCACAGTTGGATAAGATGGGGTTCAC AATTATCAGAAAATGCATCAGTGCCGTTGAAACACGAGGTATAAATGACCAAGGATTGTACAGAGTTGTG GGGGTGAGTTCAAAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAACATGCAATGAGGTGGACC TGGAGAATTCTGCAGATTGGGAAGTGAAGACAATAACAAGTGCCTTGAAACAGTATTTGAGGAGTCTTCC AGAGCCTCTCATGACCTATGAGTTACATGGAGATTTCATTGTTCCAGCCAAAAGCGGCAGCCCAGAATCT CGTGTTAATGCGATCCATTTCTTGGTACACAAACTGCCAGAGAAGAATAAAGAGATGTTGGATATTTTGG TGAAACACTTAACAAATGTTTCAAATCACTCCAAGCAGAACCTGATGACTGTGGCAAACTTAGGAGTGGT GTTTGGACCAACTCTGATGAGGCCACAGGAAGAAACTGTCGCTGCCCTCATGGACTTGAAGTTTCAGAAT ATTGTTGTGGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGGACGCCGCCCGATACTACATTCCCTG AGCCCACCTGCCTGTCAGCATCACCCCCAAATGCGCCACCAAGGCAGTCGAAGAGACAAGGCCAGAGAAC CAAGAGGCCCGTGGCCGTCTACAATCTTTGTCTGGAGCTGGAAGATGGTGACAATCCTTACCCTTCCAAG GAGGACACCCCTACCAGCAGTCTGGACTCACTTTCCTCCCCGTCTCCCGTGACTACAGCTGTCCCTGGGC CTCCTGGACCAGACAAAAACCACCTTCTGGCAGATGGAGGGAGCTTTGGAGACTGGGCATCCACTATCCC AGGCCAGACCCGATCGTCTATGGTCCAGTGGCTTAACCCACAGTCTCCAACCACAACAAGCTCCAACTCA GCTGTGACACCTCTTTCACCCGGGTCGTCCCCTTTCCCCTTTTCTCCTCCTGCTACTGTAGCGGACAAGC CACCTGAAAGCATCCGCAGTCGGAAGGCTCGAGCCGTGTATCCGTGTGAAGCAGAACACAGCTCGGAATT ATCTTTTGAAATAGGAGCAATTTTTGAGGATGTACAAACCTCCAGGGAACCTGGCTGGCTAGAAGGGACT CTGAACGGCAAGAGGGGGCTGATTCCACAGAACTACGTCAAGCTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216597 representing NM_024605
Red=Cloning site Green=Tags(s) MGLQPLEFSDCYLDSPWFRERIRAHEAELERTNKFIKELIKDGKNLIAATKSLSVAQRKFAHSLRDFKFE FIGDAVTDDERCIDASLREFSNFLKNLEEQREIMALSVTETLIKPLEKFRKEQLGAVKEEKKKFDKETEK NYSLIDKHLNLSAKKKDSHLQEADIQVEQNRQHFYELSLEYVCKLQEIQERKKFEFVEPMLSFFQGMFTF YHQGHELAKDFNHYKMELQINIQNTRNRFEGTRSEVEELMNKIRQNPKDHKRASQFTAEGYLYVQEKRPA PFGSSWVKHYCMYRKAAKKFNMIPFEHRSGGKLGDGEVFFLKECTKRHTDSIDRRFCFDIEAADRPGVSL TMQAFSEEERKQWLEALGGKEALSHSFNTAIIPRPEGNAQLDKMGFTIIRKCISAVETRGINDQGLYRVV GVSSKVQRLLSMLMDVKTCNEVDLENSADWEVKTITSALKQYLRSLPEPLMTYELHGDFIVPAKSGSPES RVNAIHFLVHKLPEKNKEMLDILVKHLTNVSNHSKQNLMTVANLGVVFGPTLMRPQEETVAALMDLKFQN IVVEILIENHEKIFRTPPDTTFPEPTCLSASPPNAPPRQSKRQGQRTKRPVAVYNLCLELEDGDNPYPSK EDTPTSSLDSLSSPSPVTTAVPGPPGPDKNHLLADGGSFGDWASTIPGQTRSSMVQWLNPQSPTTTSSNS AVTPLSPGSSPFPFSPPATVADKPPESIRSRKARAVYPCEAEHSSELSFEIGAIFEDVQTSREPGWLEGT LNGKRGLIPQNYVKLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024605 |
| ORF Size | 2358 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024605.4 |
| RefSeq Size | 3073 bp |
| RefSeq ORF | 2361 bp |
| Locus ID | 79658 |
| UniProt ID | A1A4S6 |
| Domains | SH3 |
| MW | 89.2 kDa |
| Gene Summary | GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216597L1 | Lenti-ORF clone of ARHGAP10 (Myc-DDK-tagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 7,700.00 |
|
| RC216597L2 | Lenti-ORF clone of ARHGAP10 (mGFP-tagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 7,700.00 |
|
| RC216597L3 | Lenti-ORF clone of ARHGAP10 (Myc-DDK-tagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 7,700.00 |
|
| RC216597L4 | Lenti-ORF clone of ARHGAP10 (mGFP-tagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 7,700.00 |
|
| RG216597 | ARHGAP10 (tGFP-tagged) - Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 10,232.00 |
|
| SC112115 | ARHGAP10 (untagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 3,600.00 |
|
| SC318270 | ARHGAP10 (untagged)-Human Rho GTPase activating protein 10 (ARHGAP10) |
CNY 15,200.00 |
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