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alpha 2C Adrenergic Receptor (ADRA2C) (NM_000683) Human Tagged ORF Clone
CAT＃: RC216418
ADRA2C (Myc-DDK-tagged)-Human adrenergic, alpha-2C-, receptor (ADRA2C)
ORF Plasmid: tGFP
"NM_000683" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADRA2L2; ADRA2RL2; ADRARL2; ALPHA2CAR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216418 representing NM_000683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCCCGGCGCTGGCGGCGGCGCTGGCGGTGGCGGCAGCGGCGGGCCCCAATGCGAGCGGCGCGG GCGAGAGGGGCAGCGGCGGGGTTGCCAATGCCTCGGGGGCTTCCTGGGGGCCGCCGCGCGGCCAGTACTC GGCGGGCGCGGTGGCAGGGCTGGCTGCCGTGGTGGGCTTCCTCATCGTCTTCACCGTGGTGGGCAACGTG CTGGTGGTGATCGCCGTGCTGACCAGCCGGGCGCTGCGCGCGCCACAGAACCTCTTCCTGGTGTCGCTGG CCTCGGCCGACATCCTGGTGGCCACGCTGGTCATGCCCTTCTCGTTGGCCAACGAGCTCATGGCCTACTG GTACTTCGGGCAGGTGTGGTGCGGCGTGTACCTGGCGCTCGATGTGCTGTTTTGCACCTCGTCGATCGTG CATCTGTGTGCCATCAGCCTGGACCGCTACTGGTCGGTGACGCAGGCCGTCGAGTACAACCTGAAGCGCA CACCACGCCGCGTCAAGGCCACCATCGTGGCCGTGTGGCTCATCTCGGCCGTCATCTCCTTCCCGCCGCT GGTCTCGCTCTACCGCCAGCCCGACGGCGCCGCCTACCCGCAGTGCGGCCTCAACGACGAGACCTGGTAC ATCCTGTCCTCCTGCATCGGCTCCTTCTTCGCGCCCTGCCTCATCATGGGCCTGGTCTACGCGCGCATCT ACCGAGTGGCCAAGCTGCGCACGCGCACGCTCAGCGAGAAGCGCGCCCCCGTGGGCCCCGACGGTGCGTC CCCGACTACCGAAAACGGGCTGGGCGCGGCGGCAGGCGCAGGCGAGAACGGGCACTGCGCGCCCCCGCCC GCCGACGTGGAGCCGGACGAGAGCAGCGCAGCGGCCGAGAGGCGGCGGCGCCGGGGCGCGTTGCGGCGGG GCGGGCGGCGGCGAGCGGGCGCCGAGGGGGGCGCGGGCGGTGCGGACGGGCAGGGGGCGGGGCCGGGGGC GGCTGAGTCGGGGGCGCTGACCGCCTCCAGGTCCTCGGGGCCCGGTGGCCGCCTGTCGCGCGCCAGCTCG CGCTCCGTCGAGTTCTTCCTGTCGCGCCGGCGCCGGGCGCGCAGCAGCGTGTGCCGCCGCAAGGTGGCCC AGGCGCGCGAGAAGCGCTTCACCTTTGTGCTGGCTGTGGTCATGGGTGTGTTCGTGCTCTGCTGGTTCCC CTTCTTCTTCAGCTACAGCCTGTACGGCATCTGCCGCGAGGCCTGCCAGGTGCCCGGCCCGCTCTTCAAG TTCTTCTTCTGGATCGGCTACTGCAACAGCTCGCTCAACCCGGTCATCTACACGGTCTTCAACCAGGATT TCCGGCGATCCTTTAAGCACATCCTCTTCCGACGGAGGAGAAGGGGCTTCAGGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216418 representing NM_000683
Red=Cloning site Green=Tags(s) MASPALAAALAVAAAAGPNASGAGERGSGGVANASGASWGPPRGQYSAGAVAGLAAVVGFLIVFTVVGNV LVVIAVLTSRALRAPQNLFLVSLASADILVATLVMPFSLANELMAYWYFGQVWCGVYLALDVLFCTSSIV HLCAISLDRYWSVTQAVEYNLKRTPRRVKATIVAVWLISAVISFPPLVSLYRQPDGAAYPQCGLNDETWY ILSSCIGSFFAPCLIMGLVYARIYRVAKLRTRTLSEKRAPVGPDGASPTTENGLGAAAGAGENGHCAPPP ADVEPDESSAAAERRRRRGALRRGGRRRAGAEGGAGGADGQGAGPGAAESGALTASRSSGPGGRLSRASS RSVEFFLSRRRRARSSVCRRKVAQAREKRFTFVLAVVMGVFVLCWFPFFFSYSLYGICREACQVPGPLFK FFFWIGYCNSSLNPVIYTVFNQDFRRSFKHILFRRRRRGFRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000683 |
| ORF Size | 1386 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000683.3, NP_000674.2 |
| RefSeq Size | 1958 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 152 |
| UniProt ID | P18825 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 49.3 kDa |
| Gene Summary | Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216418L1 | Lenti ORF clone of Human adrenergic, alpha-2C-, receptor (ADRA2C), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC216418L2 | Lenti ORF clone of Human adrenergic, alpha-2C-, receptor (ADRA2C), mGFP tagged |
CNY 6,560.00 |
|
| RC216418L3 | Lenti ORF clone of Human adrenergic, alpha-2C-, receptor (ADRA2C), Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC216418L4 | Lenti ORF clone of Human adrenergic, alpha-2C-, receptor (ADRA2C), mGFP tagged |
CNY 6,560.00 |
|
| RG216418 | ADRA2C (tGFP-tagged) - Human adrenergic, alpha-2C-, receptor (ADRA2C) |
CNY 7,088.00 |
|
| SC124091 | ADRA2C (untagged)-Human adrenergic, alpha-2C-, receptor (ADRA2C) |
CNY 5,488.00 |
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