ZNF286 (ZNF286A) (NM_020652) Human Tagged ORF Clone

CAT#: RC216382

ZNF286A (Myc-DDK-tagged)-Human zinc finger protein 286A (ZNF286A), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_020652" in other vectors (4)

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CNY 3,880.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ZNF286A mouse monoclonal antibody, clone OTI7D2 (formerly 7D2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ZNF286
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216382 representing NM_020652
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAGATTTGGCTGAAATGCCTGAGAAAGGAGCTCTGTCTTCCCAGGATTCTCCCCATTTCCAAG
AGAAGAGCACAGAAGAGGGAGAAGTGGCTGCTCTGCGCCTCACGGCCAGATCCCAGGAAACAGTGACATT
CAAGGATGTGGCCATGGACTTTACACCAGAGGAGTGGGGGAAGCTGGATCCTGCACAAAGGGATGTGATG
CTGGAGAACTATAGGAACCTAGTCTCACTTTGGCTTCCAGTTTCCAAACCTGAGAGCTACAACTTGGAGA
ATGGAAAAGAACCATTGAAGCTTGAGAGAAAAGCCCCCAAAAGCAGCTATTCAGACATGGAGACTAGACC
ACAGAGCAAGGATTCAACTTCAGTGCAAGATTTTTCCAAAGCAGAATCATGCAAAGTTGCAATAATAGAC
AGACTGACACGGAATAGTGTCTATGACTCTAACTTGGAAGCAGCCCTTGAATGTGAAAATTGGTTAGAGA
ATCAGCAAGGAAATCAGGAGAGACATTTGAGAGAAATGTTCACTCACATGAATTCACTCTCTGAGGAAAC
AGACCATAAGCATGATGTATACTGGAAAAGCTTCAATCAGAAATCTGTCCTTATCACTGAAGACAGAGTT
CCCAAAGGATCTTATGCCTTCCATACACTTGAAAAAAGCTTGAAACAAAAATCAAACTTAATGAAAAAGC
AGAGAACTTATAAAGAGAAAAAACCTCATAAATGTAATGATTGTGGTGAACTCTTCACCTACCATTCAGT
GCTTATTCGACACCAGAGAGTCCATACTGGAGAGAAACCCTATACCTGCAATGAATGTGGGAAATCTTTT
AGCCACAGAGCTAATTTAACTAAACACCAGAGAACTCATACTAGAATTCTCTTTGAATGCAGTGAATGCA
AGAAAACCTTCACAGAAAGCTCATCCCTTGCAACACATCAGAGAATTCACGTTGGAGAGAGACCTTATGA
ATGCAATGAATGTGGGAAAGGTTTTAATCGAAGTACACATCTTGTGCAGCATCAGTTGATTCATACTGGA
GTGAAGCCTTATGAATGTAATGAATGTGATAAAGCTTTTATTCATTCATCAGCACTCATTAAACATCAAA
GAACTCATACTGGAGAGAAACCTTATAAATGTCAAGAATGTGGGAAAGCCTTTAGCCATTGCTCATCCCT
AACTAAGCATCAGAGAGTTCATACTGGAGAAAAGCCATATGAATGCAGTGAATGTGGAAAAACTTTTAGT
CAGAGCACACATCTTGTTCAACATCAGAGAATTCACACTGGAGAGAAACCCTATGAGTGTAATGAATGTG
GGAAAACCTTCAGCCGGAGCTCCAATTTTGCTAAACATCAAAGAATTCATATTGGAAAGAAACCGTACAA
ATGTAGCGAGTGTGGAAAAGCCTTCATTCATTCATCAGCTCTTATTCAACATCAGAGAACTCATACCGGA
GAGAAACCCTTTAGATGTAATGAGTGTGGGAAAAGCTTTAAGTGCAGTTCATCTCTCATCAGACATCAAA
GAGTTCACACTGAAGAGCAACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216382 representing NM_020652
Red=Cloning site Green=Tags(s)

METDLAEMPEKGALSSQDSPHFQEKSTEEGEVAALRLTARSQETVTFKDVAMDFTPEEWGKLDPAQRDVM
LENYRNLVSLWLPVSKPESYNLENGKEPLKLERKAPKSSYSDMETRPQSKDSTSVQDFSKAESCKVAIID
RLTRNSVYDSNLEAALECENWLENQQGNQERHLREMFTHMNSLSEETDHKHDVYWKSFNQKSVLITEDRV
PKGSYAFHTLEKSLKQKSNLMKKQRTYKEKKPHKCNDCGELFTYHSVLIRHQRVHTGEKPYTCNECGKSF
SHRANLTKHQRTHTRILFECSECKKTFTESSSLATHQRIHVGERPYECNECGKGFNRSTHLVQHQLIHTG
VKPYECNECDKAFIHSSALIKHQRTHTGEKPYKCQECGKAFSHCSSLTKHQRVHTGEKPYECSECGKTFS
QSTHLVQHQRIHTGEKPYECNECGKTFSRSSNFAKHQRIHIGKKPYKCSECGKAFIHSSALIQHQRTHTG
EKPFRCNECGKSFKCSSSLIRHQRVHTEEQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020652
ORF Size 1563 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020652.3
RefSeq Size 2515 bp
RefSeq ORF 1566 bp
Locus ID 57335
UniProt ID Q9HBT8
Domains KRAB, zf-C2H2
Protein Families Transcription Factors
MW 60 kDa
Gene Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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