KCNV2 (NM_133497) Human Tagged ORF Clone

CAT#: RC216308

  • TrueORF®

KCNV2 (Myc-DDK-tagged)-Human potassium channel, subfamily V, member 2 (KCNV2)

ORF Plasmid: DDK tGFP



  "NM_133497" in other vectors (4)

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CNY 4,056.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-OR4K3 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms Kv8.2; KV11.1; RCD3B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCAAACAGAGTGAGAGGAGACGGTCCTGGAGCTACAGGCCCTGGAACACGACGGAGAATGAGGGCA
GCCAACACCGCAGGAGCATTTGCTCCCTGGGTGCCCGTTCCGGCTCCCAGGCCAGCATCCACGGCTGGAC
AGAGGGCAACTATAACTACTACATCGAGGAAGACGAAGACGGCGAGGAGGAGGACCAGTGGAAGGACGAC
CTGGCAGAAGAGGACCAGCAGGCAGGGGAGGTCACCACCGCCAAGCCCGAGGGCCCCAGCGACCCTCCGG
CCCTGCTGTCCACGCTGAATGTGAACGTGGGTGGCCACAGCTACCAGCTGGACTACTGCGAGCTGGCCGG
CTTCCCCAAGACGCGCCTAGGTCGCCTGGCCACCTCCACCAGCCGCAGCCGCCAGCTAAGCCTGTGCGAC
GACTACGAGGAGCAGACAGACGAATACTTCTTCGACCGCGACCCGGCCGTCTTCCAGCTGGTCTACAATT
TCTACCTGTCCGGGGTGCTGCTGGTGCTCGACGGGCTGTGTCCGCGCCGCTTCCTGGAGGAGCTGGGCTA
CTGGGGCGTGCGGCTCAAGTACACGCCACGCTGCTGCCGCATCTGCTTCGAGGAGCGGCGCGACGAGCTG
AGCGAACGGCTCAAGATCCAGCACGAGCTGCGCGCGCAGGCGCAGGTCGAGGAGGCGGAGGAACTCTTCC
GCGACATGCGCTTCTACGGCCCGCAGCGGCGCCGCCTCTGGAACCTCATGGAGAAGCCGTTCTCCTCGGT
GGCCGCCAAGGCCATCGGGGTGGCCTCCAGCACCTTCGTGCTCGTCTCCGTGGTGGCGCTGGCGCTCAAC
ACCGTGGAGGAGATGCAGCAGCACTCGGGGCAGGGCGAGGGCGGCCCAGACCTGCGGCCCATCCTGGAGC
ACGTGGAGATGCTGTGCATGGGCTTCTTCACGCTCGAGTACCTGCTGCGCCTAGCCTCCACGCCCGACCT
GAGGCGCTTCGCGCGCAGCGCCCTCAACCTGGTGGACCTGGTGGCCATCCTGCCGCTCTACCTTCAGCTG
CTGCTCGAGTGCTTCACGGGCGAGGGCCACCAACGCGGCCAGACGGTGGGCAGCGTGGGTAAGGTGGGTC
AGGTGTTGCGCGTCATGCGCCTCATGCGCATCTTCCGCATCCTCAAGCTGGCGCGCCACTCCACCGGACT
GCGTGCCTTCGGCTTCACGCTGCGCCAGTGCTACCAGCAGGTGGGCTGCCTGCTGCTCTTCATCGCCATG
GGCATCTTCACTTTCTCTGCGGCTGTCTACTCTGTGGAGCACGATGTGCCCAGCACCAACTTCACTACCA
TCCCCCACTCCTGGTGGTGGGCCGCGGTGAGCATCTCCACCGTGGGCTACGGAGATATGTACCCAGAGAC
CCACCTGGGCAGGTTTTTTGCCTTCCTCTGCATTGCTTTTGGGATCATTCTCAACGGGATGCCCATTTCC
ATCCTCTACAACAAGTTTTCTGATTACTACAGCAAGCTGAAGGCTTATGAGTATACCACCATACGCAGGG
AGAGGGGAGAGGTGAACTTCATGCAGAGAGCCAGAAAGAAGATAGCTGAGTGTTTGCTTGGAAGCAACCC
ACAGCTCACCCCAAGACAAGAGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216308 protein sequence
Red=Cloning site Green=Tags(s)

MLKQSERRRSWSYRPWNTTENEGSQHRRSICSLGARSGSQASIHGWTEGNYNYYIEEDEDGEEEDQWKDD
LAEEDQQAGEVTTAKPEGPSDPPALLSTLNVNVGGHSYQLDYCELAGFPKTRLGRLATSTSRSRQLSLCD
DYEEQTDEYFFDRDPAVFQLVYNFYLSGVLLVLDGLCPRRFLEELGYWGVRLKYTPRCCRICFEERRDEL
SERLKIQHELRAQAQVEEAEELFRDMRFYGPQRRRLWNLMEKPFSSVAAKAIGVASSTFVLVSVVALALN
TVEEMQQHSGQGEGGPDLRPILEHVEMLCMGFFTLEYLLRLASTPDLRRFARSALNLVDLVAILPLYLQL
LLECFTGEGHQRGQTVGSVGKVGQVLRVMRLMRIFRILKLARHSTGLRAFGFTLRQCYQQVGCLLLFIAM
GIFTFSAAVYSVEHDVPSTNFTTIPHSWWWAAVSISTVGYGDMYPETHLGRFFAFLCIAFGIILNGMPIS
ILYNKFSDYYSKLKAYEYTTIRRERGEVNFMQRARKKIAECLLGSNPQLTPRQEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133497
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133497.4
RefSeq Size 2174 bp
RefSeq ORF 1638 bp
Locus ID 169522
UniProt ID Q8TDN2
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 62.5 kDa
Gene Summary Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium voltage-gated channel subfamily V. This member is identified as a 'silent subunit', and it does not form homomultimers, but forms heteromultimers with several other subfamily members. Through obligatory heteromerization, it exerts a function-altering effect on other potassium channel subunits. This protein is strongly expressed in pancreas and has a weaker expression in several other tissues. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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