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KCNT2 (NM_198503) Human Tagged ORF Clone
CAT＃: RC216225
KCNT2 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 2 (KCNT2)
ORF Plasmid: tGFP
"NM_198503" in other vectors (6)
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CNY 11,856.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EIEE57; KCa4.2; SLICK; SLO2.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216225 representing NM_198503
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGATTTGGAGAGCGAAGTGCCCCCTCTGCCTCCCAGGTACAGGTTTCGAGATTTGCTGCTAGGGG ACCAAGGATGGCAAAACGACGACAGGGTACAAGTTGAATTCTATATGAATGAAAATACATTTAAAGAAAG ACTAAAATTATTTTTCATAAAAAACCAGAGATCAAGTCTAAGGATACGCCTGTTCAATTTTTCTCTCAAA TTACTAAGCTGCTTATTATACATAATCCGAGTACTACTAGAAAACCCTTCACAAGGAAATGAATGGTCTC ATATCTTTTGGGTGAACAGAAGTCTACCTTTGTGGGGCTTACAGGTTTCAGTGGCATTGATAAGTCTGTT TGAAACAATATTACTTGGTTATCTTAGTTATAAGGGAAACATCTGGGAACAGATTTTACGAATACCCTTC ATCTTGGAAATAATTAATGCAGTTCCCTTCATTATCTCAATATTCTGGCCTTCCTTAAGGAATCTATTTG TCCCAGTCTTTCTGAACTGTTGGCTTGCCAAACATGCCTTGGAAAATATGATTAATGATCTACACAGAGC CATTCAGCGTACACAGTCTGCAATGTTTAATCAAGTTTTGATTTTAATATCTACATTACTATGCCTTATC TTCACCTGCATTTGTGGGATCCAACATCTGGAACGAATAGGAAAGAAGCTGAATCTCTTTGACTCCCTTT ATTTCTGCATTGTGACGTTTTCTACTGTGGGCTTCGGGGATGTCACTCCTGAAACATGGTCCTCCAAGCT TTTTGTAGTTGCTATGATTTGTGTTGCTCTTGTGGTTCTACCCATACAGTTTGAACAGCTGGCTTATTTG TGGATGGAGAGACAAAAGTCAGGAGGAAACTATAGTCGACATAGAGCTCAAACTGAAAAGCATGTCGTCC TGTGTGTCAGCTCACTGAAGATTGATTTACTTATGGATTTTTTAAATGAATTCTATGCTCATCCTAGGCT CCAGGATTATTATGTGGTGATTTTGTGTCCTACTGAAATGGATGTACAGGTTCGAAGGGTACTGCAGATT CCAATGTGGTCCCAACGAGTTATCTACCTTCAAGGTTCAGCCCTTAAAGATCAAGACCTATTGAGAGCAA AGATGGATGACGCTGAGGCCTGTTTTATTCTCAGTAGCCGTTGTGAAGTGGATAGGACATCATCTGATCA CCAAACAATTTTGAGAGCATGGGCTGTGAAAGATTTTGCTCCAAATTGTCCTTTGTATGTCCAGATATTA AAGCCTGAAAATAAATTTCACATCAAATTTGCTGATCATGTTGTTTGTGAAGAAGAGTTTAAATACGCCA TGTTAGCTTTAAACTGTATATGCCCAGCAACATCTACACTTATTACACTACTGGTTCATACCTCTAGAGG GCAAGAAGGCCAGCAATCGCCAGAACAATGGCAGAAGATGTACGGTAGATGCTCCGGGAATGAAGTCTAC CACATTGTTTTGGAAGAAAGTACATTTTTTGCTGAATATGAAGGAAAGAGTTTTACATATGCCTCTTTCC ATGCACACAAAAAGTTTGGCGTCTGCTTGATTGGTGTTAGGAGGGAGGATAATAAAAACATTTTGCTGAA TCCAGGTCCTCGATACATTATGAATTCTACAGACATATGCTTTTATATTAATATTACCAAAGAAGAGAAT TCAGCATTTAAAAACCAAGACCAGCAGAGAAAAAGCAATGTGTCCAGGTCGTTTTATCATGGACCTTCCA GATTACCTGTACATAGCATAATTGCCAGCATGGGTACTGTGGCTATAGACTTGCAAGATACAAGCTGTAG ATCAGCAAGTGGCCCTACCCTGTCTCTTCCTACAGAGGGAAGCAAAGAAATAAGAAGACCTAGCATTGCT CCTGTTTTAGAGGTTGCAGATACATCATCGATTCAAACATGTGATCTTCTAAGTGACCAATCAGAAGATG AAACTACACCAGATGAAGAAATGTCTTCAAACTTAGAGTATGCTAAAGGTTACCCACCTTATTCTCCATA TATAGGAAGTTCACCCACTTTTTGTCATCTCCTTCATGAAAAAGTACCATTTTGCTGCTTAAGATTAGAC AAGAGTTGCCAACATAACTACTATGAGGATGCAAAAGCCTATGGATTCAAAAATAAACTAATTATAGTTG CAGCTGAAACAGCTGGAAATGGATTATATAACTTTATTGTTCCTCTCAGGGCATATTATAGACCAAAGAA AGAACTTAATCCCATAGTACTGCTATTGGATAACCCGCCAGATATGCATTTTCTGGATGCAATCTGTTGG TTTCCAATGGTTTACTACATGGTGGGCTCTATTGACAACCTAGATGACTTACTCAGGTGTGGAGTGACTT TTGCTGCTAATATGGTGGTTGTGGATAAAGAGAGCACCATGAGTGCCGAGGAAGACTACATGGCAGATGC CAAAACCATTGTGAACGTGCAGACACTCTTCAGGTTGTTTTCCAGTCTCAGTATTATCACAGAGCTAACT CACCCCGCCAACATGAGATTCATGCAATTCAGAGCCAAAGACTGTTACTCTCTTGCTCTTTCAAAACTGG AAAAGAAAGAACGGGAGAGAGGCTCTAACTTGGCCTTTATGTTTCGACTGCCTTTTGCTGCTGGGAGGGT GTTTAGCATCAGTATGTTGGACACTCTGCTGTATCAGTCATTTGTGAAGGATTATATGATTTCTATCACG AGACTTCTGTTGGGACTGGACACTACACCAGGATCTGGGTTTCTTTGTTCTATGAAAATCACTGCAGATG ACTTATGGATCAGAACTTATGCCAGACTTTATCAGAAGTTGTGTTCTTCTACTGGAGATGTTCCCATTGG AATCTACAGGACTGAGTCTCAGAAACTTACTACATCTGAGTCTCAAATATCTATCAGTGTAGAAGAGTGG GAAGACACCAAAGACTCCAAAGAACAAGGGCACCACCGCAGCAACCACCGCAACTCAACATCCAGTGACC AGTCGGACCATCCCTTGCTGCGGAGAAAAAGCATGCAGTGGGCCCGAAGACTGAGCAGAAAAGGCCCAAA ACACTCTGGTAAAACAGCTGAAAAAATAACCCAGCAGCGACTGAACCTCTACAGGAGGTCAGAAAGACAA GAGCTTGCTGAACTTGTGAAAAATAGAATGAAACACTTGGGTCTTTCTACAGTGGGATATGATGAAATGA ATGATCATCAAAGTACCCTCTCCTACATCCTGATTAACCCATCTCCAGATACCAGAATAGAGCTGAATGA TGTTGTATACTTAATTCGACCAGATCCACTGGCCTACCTTCCAAACAGTGAGCCCAGTCGAAGAAACAGC ATCTGCAATGTCACTGGTCAAGATTCTCGGGAGGAAACTCAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216225 representing NM_198503
Red=Cloning site Green=Tags(s) MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLK LLSCLLYIIRVLLENPSQGNEWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRIPF ILEIINAVPFIISIFWPSLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLI FTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYL WMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQI PMWSQRVIYLQGSALKDQDLLRAKMDDAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQIL KPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKMYGRCSGNEVY HIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLIGVRREDNKNILLNPGPRYIMNSTDICFYINITKEEN SAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRSASGPTLSLPTEGSKEIRRPSIA PVLEVADTSSIQTCDLLSDQSEDETTPDEEMSSNLEYAKGYPPYSPYIGSSPTFCHLLHEKVPFCCLRLD KSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICW FPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELT HPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISIT RLLLGLDTTPGSGFLCSMKITADDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQISISVEEW EDTKDSKEQGHHRSNHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQ ELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRIELNDVVYLIRPDPLAYLPNSEPSRRNS ICNVTGQDSREETQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198503 |
| ORF Size | 3405 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198503.2, NP_940905.2 |
| RefSeq Size | 5909 bp |
| RefSeq ORF | 3408 bp |
| Locus ID | 343450 |
| UniProt ID | Q6UVM3 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 130.3 kDa |
| Gene Summary | Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. Activated by high intracellular sodium and chloride levels (PubMed:14684870, PubMed:16687497, PubMed:29069600). Channel activity is inhibited by ATP and by inhalation anesthetics, such as isoflurane (PubMed:16687497) (By similarity). Inhibited upon stimulation of G-protein coupled receptors, such as CHRM1 and GRM1 (PubMed:16687497).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216225L1 | Lenti ORF clone of Human potassium channel, subfamily T, member 2 (KCNT2), Myc-DDK-tagged |
CNY 14,256.00 |
|
| RC216225L2 | Lenti ORF clone of Human potassium channel, subfamily T, member 2 (KCNT2), mGFP tagged |
CNY 13,210.00 |
|
| RC216225L3 | Lenti ORF clone of Human potassium channel, subfamily T, member 2 (KCNT2), Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC216225L4 | Lenti ORF clone of Human potassium channel, subfamily T, member 2 (KCNT2), mGFP tagged |
CNY 13,210.00 |
|
| RG216225 | KCNT2 (tGFP-tagged) - Human potassium channel, subfamily T, member 2 (KCNT2) |
CNY 13,456.00 |
|
| SC121661 | KCNT2 (untagged)-Human potassium channel, subfamily T, member 2 (KCNT2) |
CNY 7,912.00 |
