beta 1 Spectrin (SPTB) (NM_001024858) Human Tagged ORF Clone
CAT#: RC216157
- TrueORF®
SPTB (Myc-DDK-tagged)-Human spectrin, beta, erythrocytic (SPTB), transcript variant 1
ORF Plasmid: tGFP
"NM_001024858" in other vectors (2)
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CNY 15,368.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EL3; HS2; HSPTB1; SPH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216157 representing NM_001024858
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCGGCCACAGAGTTTGAAAATGTGGGCAACCAGCCACCTTACAGCAGGATCAATGCCCGCTGGG ACGCCCCAGACGACGAGCTGGATAATGACAACAGCTCAGCCAGGCTCTTTGAGAGGTCCCGGATAAAGGC CTTGGCAGATGAGCGGGAAGTTGTTCAGAAAAAGACCTTCACGAAATGGGTGAACTCGCACCTGGCTCGA GTGTCCTGCCGCATCACCGATCTCTACAAGGACCTGCGGGATGGGCGCATGCTCATCAAGCTGCTGGAGG TGCTCTCTGGAGAGATGCTGCCAAAGCCCACCAAGGGGAAGATGCGCATCCACTGCCTGGAGAATGTGGA CAAGGCTCTCCAGTTCCTCAAGGAGCAGCGTGTACACCTGGAGAACATGGGCTCCCACGACATTGTAGAT GGCAACCACCGCCTGGTCCTGGGCCTCATCTGGACCATCATCCTCCGCTTCCAGATTCAGGACATTGTGG TCCAAACTCAGGAAGGTCGTGAAACACGCTCAGCCAAGGATGCGTTGCTGTTGTGGTGTCAGATGAAGAC GGCAGGCTACCCTCATGTTAATGTCACCAACTTTACCTCCAGCTGGAAGGATGGCTTGGCCTTTAATGCC CTGATACACAAGCACCGGCCCGACCTGATCGACTTTGATAAGCTGAAGGACTCCAATGCCCGGCACAACC TGGAGCACGCATTCAATGTGGCTGAGCGCCAGCTGGGCATCATCCCGCTCCTCGACCCCGAAGATGTCTT TACGGAAAACCCTGATGAGAAATCCATCATCACCTATGTGGTGGCCTTTTACCACTACTTCTCCAAGATG AAGGTGCTGGCAGTGGAGGGCAAGCGTGTCGGCAAGGTTATTGACCATGCCATTGAGACTGAGAAGATGA TTGAAAAGTACAGCGGGCTAGCCTCGGACCTGCTCACCTGGATCGAGCAGACCATCACTGTCCTGAACAG CCGCAAGTTTGCCAACTCGCTGACGGGCGTCCAGCAGCAGCTGCAGGCCTTCAGCACCTACCGCACCGTG GAGAAGCCGCCCAAGTTTCAAGAGAAGGGGAATCTGGAAGTTCTACTTTTTACCATCCAGTCCCGGATGA GAGCCAACAATCAGAAAGTGTACACACCCCACGATGGGAAACTAGTGTCTGACATCAACAGGGCCTGGGA AAGCCTGGAGGAAGCTGAGTATCGGCGGGAGCTGGCCCTGAGAAATGAGCTCATTCGGCAGGAGAAGCTA GAGCAACTGGCCCGGCGCTTTGACCGGAAGGCCGCAATGAGAGAGACCTGGCTCAGTGAAAACCAGCGCC TCGTGGCCCAGGATAACTTTGGGTATGACCTGGCAGCTGTGGAGGCCGCCAAGAAGAAGCATGAGGCCAT CGAGACCGACACGGCTGCCTACGAGGAGCGGGTGAGAGCCCTGGAGGACCTGGCTCAGGAGCTGGAGAAA GAGAACTACCATGACCAGAAGCGCATCACGGCCCGCAAGGACAATATACTGCGCCTATGGAGCTACCTGC AGGAGCTGCTGCAGTCCCGGCGCCAGAGGCTCGAGACCACCCTGGCACTGCAGAAGCTCTTCCAGGACAT GCTGCACAGCATCGACTGGATGGATGAGATCAAGGCTCACCTCTTGTCTGCCGAGTTTGGGAAGCACTTG TTGGAGGTTGAAGACCTGCTACAGAAGCACAAGTTGATGGAAGCTGACATCGCCATCCAAGGGGACAAAG TGAAGGCCATCACCGCAGCCACCCTGAAGTTCACCGAGGGGAAAGGGTACCAGCCTTGTGACCCCCAGGT CATCCAGGACCGCATCAGCCACTTGGAGCAGTGCTTTGAGGAGCTGAGCAACATGGCAGCTGGGCGGAAG GCCCAACTGGAGCAGTCCAAACGACTCTGGAAGTTCTTCTGGGAGATGGATGAGGCTGAGAGCTGGATCA AGGAGAAGGAGCAGATCTATTCTTCCCTGGACTATGGCAAAGACCTGACCAGTGTGCTCATCTTACAGCG CAAGCACAAGGCCTTTGAGGATGAGCTCCGTGGGCTGGATGCTCACCTGGAGCAGATCTTCCAGGAGGCT CATGGCATGGTTGCGCGCAAGCAGTTTGGGCACCCGCAGATCGAGGCCCGCATAAAGGAGGTGTCGGCAC AGTGGGACCAGCTGAAGGACCTGGCTGCCTTCTGCAAGAAGAACCTCCAGGATGCTGAGAACTTTTTCCA GTTCCAGGGCGATGCGGATGACCTGAAGGCTTGGCTGCAAGACGCCCACCGGCTGCTCTCTGGTGAAGAT GTGGGGCAGGACGAAGGGGCCACGCGGGCCCTGGGGAAAAAGCACAAGGACTTCCTGGAGGAGCTGGAGG AGAGCCGTGGGGTGATGGAGCACCTGGAGCAGCAGGCCCAGGGATTCCCCGAAGAGTTTCGGGATTCCCC AGATGTGACCCATCGGCTGCAGGCCCTGCGGGAGCTCTACCAACAGGTGGTGGCCCAGGCGGACCTGCGT CAGCAGAGGCTGCAGGAAGCCCTGGACCTGTACACGGTGTTCGGGGAGACAGACGCCTGTGAGCTGTGGA TGGGAGAGAAGGAGAAGTGGCTGGCCGAGATGGAAATGCCAGACACCCTGGAGGACCTGGAGGTCGTGCA GCACAGGTTCGACATCCTGGACCAGGAGATGAAGACCTTGATGACTCAGATTGATGGTGTGAACCTCGCT GCCAACAGCTTGGTAGAGAGTGGCCACCCACGCAGCAGGGAGGTGAAGCAGTACCAGGACCATCTGAACA CCAGGTGGCAGGCATTTCAGACCCTGGTGTCGGAGCGGCGGGAGGCTGTGGACTCAGCCCTCCGAGTGCA CAACTACTGCGTAGATTGCGAGGAGACCAGCAAGTGGATCACGGACAAGACAAAGGTAGTGGAGTCCACA AAAGACCTGGGGCGGGACCTGGCAGGTATCATCGCCATCCAGAGGAAGTTGTCAGGGCTGGAGCGTGACG TGGCCGCCATCCAGGCCCGTGTGGATGCCCTGGAGCGTGAGTCCCAGCAGCTGATGGACTCGCACCCTGA GCAGAAGGAGGATATTGGTCAGCGGCAAAAACACTTGGAGGAGCTGTGGCAGGGCCTGCAGCAATCCCTG CAGGGCCAGGAGGACTTGCTGGGGGAAGTCAGCCAGCTGCAGGCCTTCCTGCAGGATCTGGATGACTTCC AGGCCTGGCTCTCCATCACCCAGAAGGCTGTGGCCTCTGAGGACATGCCCGAATCCCTCCCAGAGGCTGA GCAGCTCCTGCAGCAGCATGCAGGTATCAAGGATGAGATTGACGGGCACCAAGACAGCTACCAGCGTGTT AAGGAGTCTGGGGAGAAAGTGATCCAAGGCCAGACGGACCCAGAGTATCTGCTTCTGGGCCAGCGGCTGG AGGGCCTGGATACTGGCTGGAATGCCCTGGGCAGGATGTGGGAGAGCCGCAGCCACACCCTCGCTCAGTG CCTTGGCTTCCAGGAGTTCCAGAAAGATGCCAAGCAGGCTGAAGCCATCCTCAGCAACCAGGAATACACT CTGGCTCACTTGGAGCCCCCAGACTCCCTGGAAGCTGCAGAGGCTGGGATCCGGAAGTTTGAGGATTTCT TGGGGTCTATGGAGAACAACCGGGATAAGGTCTTGAGTCCTGTGGACTCTGGAAACAAGCTGGTAGCTGA GGGAAACCTATACTCAGACAAGATCAAGGAGAAGGTGCAGCTGATTGAGGACAGGCACAGGAAGAACAAC GAGAAGGCCCAGGAGGCCTCTGTCCTACTGAGAGACAACCTGGAGCTACAGAACTTCCTCCAGAACTGCC AGGAGCTCACTCTCTGGATCAACGACAAGCTGCTGACATCTCAGGATGTCTCCTATGATGAAGCACGAAA CCTTCACAATAAATGGCTAAAGCACCAGGCGTTTGTGGCAGAGCTGGCTTCCCATGAAGGGTGGCTAGAG AACATCGATGCGGAAGGAAAGCAGCTGATGGATGAGAAGCCCCAGTTTACAGCCCTGGTGTCCCAAAAGC TGGAAGCCCTGCACCGGCTCTGGGACGAGCTGCAGGCCACCACAAAGGAGAAGACCCAGCACCTCTCGGC TGCCAGGAGCTCCGACCTGCGCTTGCAGACCCATGCTGACCTCAACAAGTGGATCAGCGCCATGGAGGAC CAGCTGCGGTCAGACGACCCGGGCAAGGACCTGACCAGTGTCAATCGGATGTTGGCTAAGCTGAAGCGAG TGGAGGACCAAGTGAATGTGCGAAAAGAGGAGCTGGGGGAGCTGTTTGCCCAGGTGCCTTCAATGGGAGA GGAGGGAGGAGATGCAGACTTGAGCATCGAGAAGCGGTTCCTGGACCTCCTGGAACCCCTAGGAAGGAGG AAGAAGCAGCTGGAATCATCCAGAGCCAAGCTGCAGATCAGCCGGGACTTAGAGGATGAGACGCTTTGGG TGGAGGAGAGGCTGCCTCTGGCCCAGTCAGCCGACTATGGCACTAATCTGCAAACTGTGCAACTGTTCAT GAAGAAGAACCAGACACTGCAGAATGAGATTCTGGGCCATACGCCGCGGGTTGAGGATGTGCTGCAGAGA GGGCAGCAGCTGGTGGAGGCGGCGGAGATCGACTGCCAGGACCTTGAGGAGCGCCTGGGGCACCTGCAGA GCTCCTGGGACAGGCTGCGGGAGGCAGCGGCCGGGAGGCTGCAGCGACTGAGGGACGCCAACGAGGCACA GCAGTACTACCTGGATGCAGACGAGGCTGAGGCCTGGATTGGCGAGCAGGAGCTCTACGTCATCTCCGAT GAGATCCCCAAGGATGAAGAGGGCGCCATTGTGATGCTGAAGCGACATTTGCGGCAGCAGCGTGCGGTGG AGGACTACGGCCGGAACATCAAGCAGCTGGCCAGCCGGGCCCAGGGCCTGCTGTCTGCAGGCCACCCTGA GGGGGAACAGATCATCAGACTTCAGGGGCAAGTGGACAAGCACTACGCAGGGCTGAAGGACGTGGCGGAA GAGCGCAAGCGCAAGCTGGAGAACATGTACCACCTGTTCCAGCTCAAGCGGGAGACCGACGACCTGGAGC AGTGGATTTCAGAAAAGGAGCTAGTGGCCTCTTCCCCGGAAATGGGGCAAGACTTTGACCACGTGACTCT TCTGCGGGACAAGTTCCGGGACTTTGCCCGGGAGACCGGGGCGATTGGGCAGGAGCGGGTGGACAATGTG AATGCCTTCATCGAGCGACTCATCGACGCGGGCCACAGCGAGGCGGCCACCATCGCCGAGTGGAAGGACG GGCTGAACGAGATGTGGGCAGACCTCCTGGAGCTCATTGACACGCGCATGCAGCTGCTGGCCGCCTCCTA TGACCTGCACCGCTACTTCTACACGGGTGCCGAGATCCTGGGCCTCATCGACGAGAAGCACCGCGAGCTG CCCGAGGACGTGGGGCTGGACGCCAGCACGGCCGAGTCCTTCCACCGGGTGCACACAGCCTTCGAGCGGG AGCTCCACCTGCTGGGTGTCCAGGTGCAGCAGTTCCAGGACGTGGCCACCCGTCTGCAGACAGCATATGC TGGGGAGAAGGCAGAGGCCATCCAGAACAAGGAGCAGGAGGTGTCTGCCGCGTGGCAGGCGCTGCTCGAT GCCTGTGCCGGGCGCCGGACCCAGCTAGTGGACACGGCGGATAAATTCCGCTTCTTCAGCATGGCCCGTG ACCTCCTCTCCTGGATGGAGAGCATCATCCGGCAGATCGAGACCCAGGAGAGGCCCAGGGATGTCTCCTC TGTGGAACTGCTCATGAAGTATCACCAGGGCATCAATGCAGAGATTGAAACCCGGAGCAAGAACTTCAGT GCCTGCCTGGAGCTTGGCGAGTCCCTGCTGCAGCGGCAGCACCAGGCCTCAGAGGAGATCCGCGAGAAAC TGCAGCAGGTGATGTCCAGGAGGAAAGAGATGAATGAGAAGTGGGAAGCCCGCTGGGAGCGGCTCCGCAT GTTGCTGGAGGTGTGCCAGTTCTCGAGGGATGCCTCTGTGGCTGAGGCGTGGCTGATTGCCCAGGAGCCC TACCTGGCCAGCGGGGACTTTGGACACACAGTGGACAGTGTGGAGAAGCTCATCAAGAGGCATGAGGCTT TTGAGAAGTCCACGGCCAGCTGGGCAGAGCGCTTTGCTGCCCTGGAGAAGCCCACCACGCTTGAGCTGAA AGAACGCCAGATTGCAGAGAGACCCGCAGAGGAGACTGGGCCTCAAGAGGAGGAAGGCGAGACAGCAGGG GAGGCTCCAGTTTCCCACCATGCGGCCACCGAGAGAACGTCCCCGGGGGAAGAAGAGGGAACGTGGCCTC AGAACCTGCAGCAGCCACCACCGCCGGGTCAGCACAAGGATGGGCAGAAATCCACTGGGGATGAGAGGCC CACCACGGAGCCCCTCTTTAAGGTCCTAGATACGCCTCTGAGCGAGGGTGATGAGCCCGCAACGCTGCCG GCCCCGCGGGACCATGGGCAGAGTGTGCAGATGGAAGGCTACCTGGGCCGCAAGCATGACCTGGAGGGGC CCAACAAGAAGGCTTCCAACAGGTCCTGGAACAACCTGTACTGTGTGCTCAGGAACAGTGAGCTAACCTT CTACAAGGATGCCAAGAACCTGGCCCTGGGGATGCCCTACCATGGGGAGGAACCCCTGGCCCTGAGACAT GCCATCTGTGAGATTGCTGCCAACTACAAGAAGAAGAAGCACGTCTTTAAGCTGAGGCTGAGTAATGGCA GCGAGTGGCTCTTCCATGGCAAGGATGAGGAGGAGATGCTGTCCTGGCTGCAGGGCGTGAGCACCGCCAT CAACGAGTCCCAGAGCATCCGCGTCAAGGCGCAGAGCCTGCCCCTGCCTTCCCTCTCCGGCCCCGACGCC AGCCTCGGCAAGAAAGACAAGGAGAAGAGATTCAGCTTCTTCCCCAAAAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216157 representing NM_001024858
Red=Cloning site Green=Tags(s) MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLAR VSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVD GNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNA LIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKM KVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTV EKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKL EQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEK ENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHL LEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRK AQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEA HGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGED VGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLR QQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLA ANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVEST KDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSL QGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRV KESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYT LAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNN EKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLE NIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMED QLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRR KKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQR GQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISD EIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAE ERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNV NAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHREL PEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLD ACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFS ACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEP YLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAG EAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLP APRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRH AICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDA SLGKKDKEKRFSFFPKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001024858 |
ORF Size | 6984 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024858.3 |
RefSeq Size | 10064 bp |
RefSeq ORF | 6987 bp |
Locus ID | 6710 |
UniProt ID | P11277 |
Protein Families | Druggable Genome |
MW | 267.6 kDa |
Gene Summary | This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
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