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Myosin IIIB (MYO3B) (NM_001083615) Human Tagged ORF Clone
CAT＃: RC216078
MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 1
ORF Plasmid: tGFP
"NM_001083615" in other vectors (4)
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CNY 9,152.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216078 representing NM_001083615
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCA CAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAA GAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCA GAATACAACATTTTGCAGTTCCTTCCTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAG CGGATCACTGTGTAGGGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCT TGTCAAAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGCC CTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAATAACATTCTTC TGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAACTCACCAGTACACGTCTGCG GAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCCTGAGGTCATTGCCTGTGAGCAGCAGTATGAC TCTTCCTATGACGCTCGCTGTGACGTCTGGTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACC CTCCCCTCTTTGACATGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACT TCATCCAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTGAAAGG CGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAAGTTCTGTTTCTGC AAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTTGCTAAAACCAGGCATGAGAG GATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAAAAATACTGCCTTGAGGATGATTTGGTCAAC CTAGAGGTTCTGGATGAGGATACAATTATCCATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACA CATATGTTGGAGACATCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTC CAGACTTTATCATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTGCTTAC CAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTGGCTCTGGGAAGACAG AAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGGCCAATAATCAGACCTTGAGAGAGAA AATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAACTCATGCACTGCCATCAATGACAACTCGAGC CGTTTTGGAAAATATCTGGAAATGATGTTTACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAAT ATCTCCTGGAAAAATCCAGAGTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATAT TTATGCTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTAGGTAC ATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACAGAAGACAATTCGAAG CAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGGTGCACTCAGTGTACAGAATTTTGGC TGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATTTCCTCTCAACATCAGACTGATAAAAGTGAG GTGCCCAATGCTGAAGCTTTGCAAAATGCTGCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGG CCCTCACCTCCCACTGTGTGGTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGC GGACGTTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCATTAAT ACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGGGGATCTTGGATATCT TTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCATAAACATCGCCAATGAGCAAATCCA GTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAGATGGAATATCAGAATGAAGGCATTGATGCTGTA CCCGTGGAATATGAGGACAACCGCCCGCTCTTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCAC TTTTGGATGAGGAAAGTCGGTTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCT ACGATGCAAATACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAG GTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCGATGTGGTTGTGGTCC TGAGAACGTCAGAAAACAAGCTTCTTCAGCAGCTCTTCTCAATCCCTCTGACCAAAACAGGTAATTTGGC CCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTTCTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAG GTGGACACTCTGGAGGTGATACGGCATCCGGAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTT ACTTCCGGTATTCTCTGATGGACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCAT TAAACCCAATGATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACA GGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTTTTGAAGAATTTGTGA AAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTGCTAGCAAAGAGAGCTGTGTGGCTAT CTTGGAAAAGTCCAGATTAGATCACTGGGTACTGGGAAAAACAAAGGTTTTTCTCAAATATTACCATGTT GAGCAATTAAATTTGCTGCTTCGAGAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGT GGCTTGGAGCCAGGAGATACAAAAGGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGG GGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAGCAGGTACGAGGGGAAGTGCCGAGGTT CAAGACTGCAGCGAGCCTGGTGACCATAAAGTTCTCAGGGGCTCTGTACATCGTAGGAGCCATTCACAAG CAGAATCCAACAATGGCCGTACACAGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAAAATGGGCATTC ACAAGCCCAGAGTTCTCCAAAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACTCGGTTTCTGGG ACTGATTTGCTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCTCTGGGGAGCCC CTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGATGTCAGCAGCC CAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAGAATATCAAGGG AGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTACTTGATGGGGAAGATGAATATTACAAATCTC TGTCACCAGTGGACTGTATCCCTGAGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCTTCATCCTCAAA AGGAGACTCTTTTGCTCAACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216078 representing NM_001083615
Red=Cloning site Green=Tags(s) MKHLYGLFHYNPMMLGLESLPDPTDTWEIIETIGKGTYGKVYKVTNKRDGSLAAVKILDPVSDMDEEIEA EYNILQFLPNHPNVVKFYGMFYKADHCVGGQLWLVLELCNGGSVTELVKGLLRCGQRLDEAMISYILYGA LLGLQHLHNNRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYD SSYDARCDVWSLGITAIELGDGDPPLFDMHPVKTLFKIPRNPPPTLLHPEKWCEEFNHFISQCLIKDFER RPSVTHLLDHPFIKGVHGKVLFLQKQLAKVLQDQKHQNPVAKTRHERMHTRRPYHVEDAEKYCLEDDLVN LEVLDEDTIIHQLQKRYADLLIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVKRASNPPHIFASADAAY QCMVTLSKDQCIVISGESGSGKTESAHLIVQHLTFLGKANNQTLREKILQVNSLVEAFGNSCTAINDNSS RFGKYLEMMFTPTGVVMGARISEYLLEKSRVIKQAAREKNFHIFYYIYAGLHHQKKLSDFRLPEEKPPRY IADETGRVMHDITSKESYRRQFEAIQHCFRIIGFTDKEVHSVYRILAGILNIGNIEFAAISSQHQTDKSE VPNAEALQNAASVLCISPEELQEALTSHCVVTRGETIIRANTVDRAADVRDAMSKALYGRLFSWIVNRIN TLLQPDENICSAGGGMNVGILDIFGFENFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYQNEGIDAV PVEYEDNRPLLDMFLQKPLGLLALLDEESRFPQATDQTLVDKFEDNLRCKYFWRPKGVELCFGIQHYAGK VLYDASGVLEKNRDTLPADVVVVLRTSENKLLQQLFSIPLTKTGNLAQTRARITVASSSLPPHFSAGKAK VDTLEVIRHPEETTNMKRQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNDDREALQFSRERVLAQLRST GILETVSIRRQGYSHRILFEEFVKRYYYLAFTAHQTPLASKESCVAILEKSRLDHWVLGKTKVFLKYYHV EQLNLLLREVIGRVVVLQAYTKGWLGARRYKRVREKREKGAIAIQSGDTSNQSSGPHSPVAAGTRGSAEV QDCSEPGDHKVLRGSVHRRSHSQAESNNGRTQTSSNSPAVTEKNGHSQAQSSPKGCDIFAGHANKHSVSG TDLLSSRICHPAPDQQGLSLWGAPQKPGSENGLAQKHRTPRRRCQQPKMLSSPEDTMYYNQLNGTLEYQG SKRKPRKLGQIKVLDGEDEYYKSLSPVDCIPEENNSAHPSFFSSSSKGDSFAQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083615 |
| ORF Size | 3942 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083615.4 |
| RefSeq Size | 5448 bp |
| RefSeq ORF | 3945 bp |
| Locus ID | 140469 |
| UniProt ID | Q8WXR4 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 148.4 kDa |
| Gene Summary | This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216078L3 | Lenti ORF clone of Human myosin IIIB (MYO3B), transcript variant 1, Myc-DDK-tagged |
CNY 15,010.00 |
|
| RC216078L4 | Lenti ORF clone of Human myosin IIIB (MYO3B), transcript variant 1, mGFP tagged |
CNY 15,010.00 |
|
| RG216078 | MYO3B (tGFP-tagged) - Human myosin IIIB (MYO3B), transcript variant 1 |
CNY 14,440.00 |
|
| SC316082 | MYO3B (untagged)-Human myosin IIIB (MYO3B), transcript variant 1 |
CNY 25,370.00 |
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