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UBE2NL (NM_001012989) Human Tagged ORF Clone
CAT＃: RC215865
UBE2NL (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2N-like (UBE2NL)
ORF Plasmid: tGFP
"NM_001012989" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Li174 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215865 representing NM_001012989
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAGCCAGTTCCTGGCATCA AAGCAGAACCAGATGAAAGCAACGCCCGTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCC CTTTGAGGGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGCAGCCCCTAAAGTA CGTTTCATGACCAAAATTTATCATCCAAATGTAGACAAGTTGGAAAGAATAAGTTTAGATATTTTGAAAG ATAAGTGGTCCCCAGCCCTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCCCAA TCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATTGAAACA GCTAGAGCATGGACTAGGCTATATGCCATGAATAGTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215865 representing NM_001012989
Red=Cloning site Green=Tags(s) MAELPHRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKV RFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQAIET ARAWTRLYAMNSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012989 |
| ORF Size | 459 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012989.2, NP_001013007.1 |
| RefSeq Size | 1190 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 389898 |
| UniProt ID | Q5JXB2 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 17.2 kDa |
| Gene Summary | This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence. [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215865L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2N-like (UBE2NL), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC215865L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2N-like (UBE2NL), mGFP tagged |
CNY 5,890.00 |
|
| RG215865 | UBE2NL (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2N-like (UBE2NL) |
CNY 4,370.00 |
|
| SC301756 | UBE2NL (untagged)-Human ubiquitin-conjugating enzyme E2N-like (UBE2NL) |
CNY 3,990.00 |
