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PPP2R3A (NM_181897) Human Tagged ORF Clone
CAT＃: RC215798
PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2
ORF Plasmid: tGFP
"NM_181897" in other vectors (6)
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CNY 3,936.00
CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PPP2R3; PR72; PR130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215798 representing NM_181897
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATCAAGGAAACATCTCTACGAAGGGACCCGGATTTAAGGGGAGAGCTAGCTTTCCTGGCAAGGG GCTGTGATTTTGTTCTCCCTTCACGGTTTAAGAAGCGGCTGAAGTCATTTCAGCAGACACAGATTCAAAA TAAACCAGAAAAGAAACCTGGAACACCACTCCCACCTCCAGCCACCTCTCCAAGTAGTCCCCGACCTCTC TCCCCGGTTCCCCATGTGAATAATGTTGTGAATGCGCCATTGTCCATAAACATTCCACGGTTCTACTTTC CTGAAGGACTCCCAGATACCTGTAGTAATCATGAACAAACTCTAAGCAGAATTGAAACTGCTTTCATGGA TATTGAAGAACAGAAAGCAGACATTTATGAAATGGGGAAAATTGCAAAGGTCTGTGGCTGTCCTCTCTAT TGGAAAGCCCCCATGTTCAGGGCTGCAGGGGGAGAGAAGACAGGATTTGTGACAGCACAGTCATTCATTG CCATGTGGAGAAAGTTGCTGAATAACCATCATGATGATGCCTCTAAATTCATCTGTCTTCTAGCAAAGCC CAACTGCAGCTCTCTAGAACAGGAGGATTTCATCCCTCTACTTCAGGATGTGGTGGATACCCACCCTGGT CTCACGTTCCTGAAAGATGCTCCAGAATTCCACTCCCGCTACATCACCACGGTTATTCAGAGAATATTCT ACACAGTCAACAGATCTTGGAGTGGAAAAATTACTTCGACAGAGATAAGAAAAAGCAACTTTTTGCAAAC CCTAGCACTTTTGGAAGAAGAGGAAGATATAAACCAAATTACAGATTACTTCTCCTATGAACATTTCTAT GTTATTTATTGTAAATTCTGGGAACTAGATACTGATCACGACCTCTACATCAGCCAGGCCGATCTGTCTC GATACAATGACCAGGCTTCATCAAGCAGGATTATTGAAAGGATATTCTCTGGTGCAGTAACAAGGGGAAA AACAATACAGAAAGAGGGAAGAATGAGCTATGCAGATTTTGTTTGGTTTTTGATCTCTGAAGAAGACAAA AGGAATCCTACCAGCATTGAGTATTGGTTCCGCTGCATGGATGTGGATGGAGACGGTGTACTCTCCATGT ATGAGCTGGAGTACTTCTATGAGGAGCAGTGTGAACGGATGGAAGCCATGGGAATTGAGCCCTTGCCATT CCATGATTTACTGTGCCAGATGCTTGACCTAGTGAAGCCAGCTGTTGATGGCAAAATAACTCTAAGAGAT CTGAAGAGGTGCAGAATGGCTCACATCTTCTATGACACTTTCTTTAATCTGGAGAAATACTTAGACCATG AACAGAGAGATCCCTTTGCGGTCCAGAAGGATGTTGAGAACGATGGGCCTGAGCCCTCAGACTGGGACCG GTTTGCCGCTGAGGAGTATGAGACGCTTGTTGCAGAGGAATCTGCCCAAGCACAATTCCAGGAAGGCTTT GAAGATTATGAAACAGATGAACCTGCCTCTCCCTCTGAATTTGGAAACAAAAGCAATAAAATATTAAGTG CAAGCCTTCCAGAGAAATGTGGAAAGCTTCAATCAGTGGATGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215798 representing NM_181897
Red=Cloning site Green=Tags(s) MMIKETSLRRDPDLRGELAFLARGCDFVLPSRFKKRLKSFQQTQIQNKPEKKPGTPLPPPATSPSSPRPL SPVPHVNNVVNAPLSINIPRFYFPEGLPDTCSNHEQTLSRIETAFMDIEEQKADIYEMGKIAKVCGCPLY WKAPMFRAAGGEKTGFVTAQSFIAMWRKLLNNHHDDASKFICLLAKPNCSSLEQEDFIPLLQDVVDTHPG LTFLKDAPEFHSRYITTVIQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFY VIYCKFWELDTDHDLYISQADLSRYNDQASSSRIIERIFSGAVTRGKTIQKEGRMSYADFVWFLISEEDK RNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPAVDGKITLRD LKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENDGPEPSDWDRFAAEEYETLVAEESAQAQFQEGF EDYETDEPASPSEFGNKSNKILSASLPEKCGKLQSVDEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181897 |
| ORF Size | 1587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181897.3 |
| RefSeq Size | 4664 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 5523 |
| UniProt ID | Q06190 |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 60.9 kDa |
| Gene Summary | This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215798L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, Myc-DDK-tagged |
CNY 6,336.00 |
|
| RC215798L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, mGFP tagged |
CNY 6,560.00 |
|
| RC215798L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC215798L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, mGFP tagged |
CNY 6,560.00 |
|
| RG215798 | PPP2R3A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2 |
CNY 5,536.00 |
|
| SC107434 | PPP2R3A (untagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2 |
CNY 3,944.00 |
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