United States
Germany
Japan
United Kingdom
China
TRIM45 (NM_025188) Human Tagged ORF Clone
CAT＃: RC215738
TRIM45 (Myc-DDK-tagged)-Human tripartite motif containing 45 (TRIM45), transcript variant 1
ORF Plasmid: tGFP
"NM_025188" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,320.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RNF99 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215738 representing NM_025188
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAAACAGAAAACCGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCACTTGGGAACT CAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGGCTCTTGCCTTGTTTGCATAC AGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCAGTAGTGGACATCCGAGGGGGAGACTCTGAC ACAAGCTCTGAGGGGTCAATATTCCAGGAACTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTT GTCCTGTATGTGATGCTCAGGTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGC CGTGAATGATGTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAGGCTCATAGGC GGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAAGGCTACAGCCGGATTGGGAA GCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGGCTGTTCTGTGAGTTCTGTGACCGGCCCGTG TGCCAGGATTGTGTGGTGGGGGAGCATCGGGAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGC ATGGGGACTCTGTGTGGGAGCTCCTCAAAGGTACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGC TCAGATCCACATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAAGACATACGGG CCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTACTGGCAGACATGCGGACTGG AGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGACTTGGAGATCCTCATCACCAAGAGGGTGGTG GTAGAACGGCTCAGGAAGCTGAACAAAGTTCAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCT TCTGTCCTCAGGAGAAAGCAGGCCAGTGCCGTGGCTATGAAATTTATGGTACGATTAATACCAAAGAGGT TGATCCAGCCAAATGTGTCCTACAAGGAGAAGACCTCCACAGAGCCCGGGAGAAACAGACGGCCTCTTTC ACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAACGTTCAAGTTGCCGTTG TCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAGGATAACAAGGATGGGACATACTACAT TTCCTACACCCCCAAGGAACCTGGCGTCTATACTGTGTGGGTCTGCATCAAAGAACAGCATGTGCAGGGC TCGCCATTCACTGTGACGGTGAGGAGAAAGCACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCT GCTCCAGCGGGGGCCAGAAAACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTG TGGCCATGGACACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAGAAATCT GAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTGGCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215738 representing NM_025188
Red=Cloning site Green=Tags(s) MSENRKPLLGFVSKLTSGTALGNSGKTHCPLCLGLFKAPRLLPCLHTVCTTCLEQLEPFSVVDIRGGDSD TSSEGSIFQELKPRSLQSQIGILCPVCDAQVDLPMGGVKALTIDHLAVNDVMLESLRGEGQGLVCDLCND REVEKRCQTCKANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSRIGKPILCPVHPAEELRLFCEFCDRPV CQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVEALEEALAQIHIINSALQKRVEAVAADVRTF SEGYIKAIEEHRDKLLKQLEDIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVV VERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYGTINTKEVDPAKCVLQGEDLHRAREKQTASF TLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYISYTPKEPGVYTVWVCIKEQHVQG SPFTVTVRRKHRPHSGVFHCCTFCSSGGQKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKS ECTWTGGQSAPRSLLRTVAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025188 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025188.2, NP_079464.1 |
| RefSeq Size | 3584 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 80263 |
| UniProt ID | Q9H8W5 |
| Domains | zf-B_box, Filamin, RING, BBC |
| MW | 64.2 kDa |
| Gene Summary | This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215738L1 | Lenti ORF clone of Human tripartite motif containing 45 (TRIM45), transcript variant 1, Myc-DDK-tagged |
CNY 6,720.00 |
|
| RC215738L2 | Lenti ORF clone of Human tripartite motif containing 45 (TRIM45), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC215738L3 | Lenti ORF clone of Human tripartite motif containing 45 (TRIM45), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC215738L4 | Lenti ORF clone of Human tripartite motif containing 45 (TRIM45), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG215738 | TRIM45 (tGFP-tagged) - Human tripartite motif containing 45 (TRIM45), transcript variant 1 |
CNY 5,920.00 |
|
| SC110258 | TRIM45 (untagged)-Human tripartite motif containing 45 (TRIM45), transcript variant 1 |
CNY 4,328.00 |
