FUBP1 (NM_003902) Human Tagged ORF Clone

CAT#: RC215674

FUBP1 (Myc-DDK-tagged)-Human far upstream element (FUSE) binding protein 1 (FUBP1)

ORF Plasmid: DDK tGFP



  "NM_003902" in other vectors (6)

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CNY 4,792.00

CNY 5,700.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


FUBP1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FBP; FUBP; hDH V
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215674 representing NM_003902
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGCGGCGGTGGTG
GTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAGATTGCAGCAAAAATTGGAGG
TGATGCAGGGACATCACTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCTTTAGAAGATGGA
GATCAACCAGATGCTAAGAAAGTTGCTCCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATC
AGCAGCAAAGCAGATCTGTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGG
CAGAGGAGGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACAGT
GGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAGCAAAACGGTTAC
TGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATGGCGATGGACCGGGAAATGCAGT
TCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTAGTCATTGGAAAAGGGGGAGAAACTATTAAACAG
CTTCAGGAACGGGCTGGAGTTAAAATGGTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAAC
CTCTTAGGATTACAGGAGACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGA
TCAAGGCGGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGATGTC
CCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAAAAAATACAAAATG
ATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCCGAAAGGATAGCACAAATAACAGG
ACCTCCAGACCGATGTCAACATGCTGCAGAAATTATTACAGACCTTCTTCGAAGTGTTCAGGCTGGTAAT
CCTGGTGGACCTGGACCTGGTGGTCGAGGAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTG
GTGGACTACAGGAATTTAATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGA
AACCATAAAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAATGCA
GATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCTCGGCAACTCATAG
AAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGGCCCCATGGTGTCCCAGG
CCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACTCCAATGGGACCATACAACCCTGCACCTTATAAT
CCTGGACCACCAGGCCCGGCTCCTCATGGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCAT
ATCCACACTGGCAGCAGCAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTG
GGCTGCTTATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCACCA
ACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAGGTTGATTATACCA
AGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCTCCGACTGGGGCTCCTCCAGGTGG
TCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTATAGACAACAAGCAGCCTATTATGCCCAGACAAGT
CCCCAGGGAATGCCACAGCATCCTCCAGCACCTCAGGGCCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215674 representing NM_003902
Red=Cloning site Green=Tags(s)

MADYSTVPPPSSGSAGGGGGGGGGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDG
DQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDS
GGLPERSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQ
LQERAGVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDV
PIPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGN
PGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNA
DPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYN
PGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAP
TTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTS
PQGMPQHPPAPQGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003902
ORF Size 1932 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003902.5
RefSeq Size 2884 bp
RefSeq ORF 1935 bp
Locus ID 8880
UniProt ID Q96AE4
Domains KH
Protein Families Stem cell - Pluripotency, Transcription Factors
MW 67.4 kDa
Gene Summary The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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