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CYP11B2 (NM_000498) Human Tagged ORF Clone
CAT＃: RC215476
CYP11B2 (Myc-DDK-tagged)-Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_000498" in other vectors (6)
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CNY 5,616.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALDOS; CPN2; CYP11B; CYP11BL; CYPXIB2; P-450C18; P450aldo; P450C18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215476 representing NM_000498
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTCAGGGCAAAGGCAGAGGTGTGCGTGGCAGCGCCCTGGCTGTCCCTGCAAAGGGCACGGGCAC TGGGCACTAGAGCCGCTCGGGCCCCTAGGACGGTGCTGCCGTTTGAAGCCATGCCCCAGCATCCAGGCAA CAGGTGGCTGAGGCTGCTGCAGATCTGGAGGGAGCAGGGTTATGAGCACCTGCACCTGGAGATGCACCAG ACCTTCCAGGAGCTGGGGCCCATTTTCAGGTACAACTTGGGAGGACCACGCATGGTGTGTGTGATGCTGC CGGAGGATGTGGAGAAGCTGCAACAGGTGGACAGCCTGCATCCCTGCAGGATGATCCTGGAGCCCTGGGT GGCCTACAGACAACATCGTGGGCACAAATGTGGCGTGTTCTTGTTGAATGGGCCTGAATGGCGCTTCAAC CGATTGCGGCTGAACCCAGATGTGCTGTCGCCCAAGGCCGTGCAGAGGTTCCTCCCGATGGTGGATGCAG TGGCCAGGGACTTCTCCCAGGCCCTGAAGAAGAAGGTGCTGCAGAACGCCCGGGGGAGCCTGACCCTGGA CGTCCAGCCCAGCATCTTCCACTACACCATAGAAGCCAGCAACTTAGCTCTTTTTGGAGAGCGGCTGGGC CTGGTTGGCCACAGCCCCAGTTCTGCCAGCCTGAACTTCCTCCATGCCCTGGAGGTCATGTTCAAATCCA CCGTCCAGCTCATGTTCATGCCCAGGAGCCTGTCTCGCTGGATCAGCCCCAAGGTGTGGAAGGAGCACTT TGAGGCCTGGGACTGCATCTTCCAGTACGGTGACAACTGTATCCAGAAAATCTACCAGGAACTGGCCTTC AACCGCCCTCAACACTACACAGGCATCGTGGCGGAGCTCCTGTTGAAGGCGGAACTGTCACTAGAAGCCA TCAAGGCCAACTCTATGGAACTCACTGCAGGGAGCGTGGACACGACAGCGTTTCCCTTGCTGATGACGCT CTTTGAGCTGGCTCGGAACCCCGACGTGCAGCAGATCCTGCGCCAGGAGAGCCTGGCCGCCGCAGCCAGC ATCAGTGAACATCCCCAGAAGGCAACCACCGAGCTGCCCTTGCTGCGGGCGGCCCTCAAGGAGACCTTGC GGCTCTACCCTGTGGGTCTGTTTTTGGAGCGAGTGGTGAGCTCAGACTTGGTGCTTCAGAACTACCACAT CCCAGCTGGGACATTGGTACAGGTTTTCCTCTACTCGCTGGGTCGCAATGCCGCCTTGTTCCCGAGGCCT GAGCGGTATAATCCCCAGCGCTGGCTAGACATCAGGGGCTCCGGCAGGAACTTCCACCACGTGCCCTTTG GCTTTGGCATGCGCCAGTGCCTCGGGCGGCGCCTGGCAGAGGCAGAGATGCTGCTGCTGCTGCACCACGT GCTGAAGCACTTCCTGGTGGAGACACTAACTCAAGAGGACATAAAGATGGTCTACAGCTTCATATTGAGG CCTGGCACGTCCCCCCTCCTCACTTTCAGAGCGATTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215476 representing NM_000498
Red=Cloning site Green=Tags(s) MALRAKAEVCVAAPWLSLQRARALGTRAARAPRTVLPFEAMPQHPGNRWLRLLQIWREQGYEHLHLEMHQ TFQELGPIFRYNLGGPRMVCVMLPEDVEKLQQVDSLHPCRMILEPWVAYRQHRGHKCGVFLLNGPEWRFN RLRLNPDVLSPKAVQRFLPMVDAVARDFSQALKKKVLQNARGSLTLDVQPSIFHYTIEASNLALFGERLG LVGHSPSSASLNFLHALEVMFKSTVQLMFMPRSLSRWISPKVWKEHFEAWDCIFQYGDNCIQKIYQELAF NRPQHYTGIVAELLLKAELSLEAIKANSMELTAGSVDTTAFPLLMTLFELARNPDVQQILRQESLAAAAS ISEHPQKATTELPLLRAALKETLRLYPVGLFLERVVSSDLVLQNYHIPAGTLVQVFLYSLGRNAALFPRP ERYNPQRWLDIRGSGRNFHHVPFGFGMRQCLGRRLAEAEMLLLLHHVLKHFLVETLTQEDIKMVYSFILR PGTSPLLTFRAIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000498 |
| ORF Size | 1509 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000498.3 |
| RefSeq Size | 2936 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 1585 |
| UniProt ID | P19099 |
| Protein Families | Druggable Genome |
| Protein Pathways | C21-Steroid hormone metabolism, Metabolic pathways |
| MW | 57.56 kDa |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215476L1 | Lenti ORF clone of Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC215476L2 | Lenti ORF clone of Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,370.00 |
|
| RC215476L3 | Lenti ORF clone of Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC215476L4 | Lenti ORF clone of Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,016.00 |
|
| RG215476 | CYP11B2 (tGFP-tagged) - Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein |
CNY 7,216.00 |
|
| SC300084 | CYP11B2 (untagged)-Human cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein |
CNY 5,632.00 |
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