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ODF1 (NM_024410) Human Tagged ORF Clone
CAT＃: RC215265
ODF1 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 1 (ODF1)
ORF Plasmid: tGFP
"NM_024410" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT133; HSPB10; ODF; ODF2; ODF27; ODFP; ODFPG; ODFPGA; ODFPGB; RT7; SODF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCACTGAGTTGTCTCTTGGACAGTGTCAGAAGGGACATAAAGAAGGTGGACAGAGAACTAAGGC AACTGAGATGCATCGACGAATTTAGCACACGGTGCCTGTGCGACTTGTATATGCACCCCTATTGCTGCTG TGACTTGCACCCATATCCGTACTGCTTGTGCTATTCCAAGCGATCACGCTCTTGCGGCCTGTGTGATCTC TACCCATGTTGCCTGTGTGATTATAAGCTTTACTGTCTGCGACCATCTCTCAGAAGTTTGGAGAGGAAAG CCATCAGAGCCATAGAAGATGAGAAGCGAGAGCTTGCCAAACTGAGAAGAACAACAAATAGAATTCTGGC TTCCTCCTGCTGTAGCAGTAACATTTTAGGATCGGTGAATGTATGCGGTTTTGAACCCGATCAAGTCAAA GTTCGAGTGAAGGATGGAAAGGTATGTGTGTCGGCTGAGCGGGAGAACAGGTACGACTGCCTTGGATCGA AAAAGTACAGCTACATGAACATCTGCAAAGAGTTCAGCTTGCCGCCCTGTGTGGATGAGAAGGATGTAAC ATACTCCTATGGGCTCGGCAGCTGTGTCAAGATCGAGTCTCCTTGCTACCCTTGCACTTCTCCTTGCAGC CCGTGCAGCCCGTGCAACCCCTGCAACCCCTGCAGCCCCTGCAACCCGTGCAGCCCATATGATCCTTGCA ACCCGTGTTATCCCTGTGGAAGCCGATTTTCCTGTAGGAAGATGATTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215265 protein sequence
Red=Cloning site Green=Tags(s) MAALSCLLDSVRRDIKKVDRELRQLRCIDEFSTRCLCDLYMHPYCCCDLHPYPYCLCYSKRSRSCGLCDL YPCCLCDYKLYCLRPSLRSLERKAIRAIEDEKRELAKLRRTTNRILASSCCSSNILGSVNVCGFEPDQVK VRVKDGKVCVSAERENRYDCLGSKKYSYMNICKEFSLPPCVDEKDVTYSYGLGSCVKIESPCYPCTSPCS PCSPCNPCNPCSPCNPCSPYDPCNPCYPCGSRFSCRKMIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024410 |
| ORF Size | 750 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024410.2, NP_077721.1 |
| RefSeq Size | 1007 bp |
| RefSeq ORF | 753 bp |
| Locus ID | 4956 |
| UniProt ID | Q14990 |
| MW | 28.4 kDa |
| Gene Summary | The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. The human outer dense fibers contains at least 10 major proteins and this gene encodes the main protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215265L3 | Lenti ORF clone of Human outer dense fiber of sperm tails 1 (ODF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215265L4 | Lenti ORF clone of Human outer dense fiber of sperm tails 1 (ODF1), mGFP tagged |
CNY 5,890.00 |
|
| RG215265 | ODF1 (tGFP-tagged) - Human outer dense fiber of sperm tails 1 (ODF1) |
CNY 4,000.00 |
|
| SC305112 | ODF1 (untagged)-Human outer dense fiber of sperm tails 1 (ODF1) |
CNY 3,990.00 |
