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ENAM (NM_031889) Human Tagged ORF Clone
CAT＃: RC215089
ENAM (Myc-DDK-tagged)-Human enamelin (ENAM)
ORF Plasmid: tGFP
"NM_031889" in other vectors (4)
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CNY 7,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADAI; AI1C; AIH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215089 representing NM_031889
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTGCTTCGGTGCAGGCTTGGAACCTCTTTTCCTAAACTAGATAACTTGGTACCAAAAGGCAAAA TGAAGATTCTCCTGGTCTTTCTAGGGCTTCTTGGTAATTCTGTTGCTATGCCAATGCACATGCCCCGAAT GCCTGGATTTAGCAGTAAAAGTGAGGAGATGATGCGGTATAATCAATTCAACTTTATGAACGGCCCACAT ATGGCACACCTGGGGCCCTTCTTTGGAAACGGTCTCCCTCAGCAATTTCCACAGTACCAGATGCCCATGT GGCCTCAGCCACCACCCAACACATGGCATCCACGGAAATCCTCAGCACCCAAACGTCATAACAAGACTGA TCAGACCCAAGAAACCCAGAAACCCAACCAGACTCAGTCAAAAAAGCCACCACAAAAGCGGCCTTTGAAG CAGCCATCACATAATCAACCTCAGCCCGAAGAGGAAGCTCAACCCCCTCAGGCATTCCCACCATTTGGAA ATGGGCTATTCCCCTATCAACAACCACCATGGCAAATTCCACAGAGGTTACCACCACCAGGTTATGGACG CCCACCAATCAGCAATGAAGAAGGGGGGAATCCTTACTTTGGATATTTTGGATATCATGGCTTTGGGGGT CGCCCTCCTTATTATTCAGAAGAAATGTTTGAACAAGATTTTGAAAAACCCAAAGAAGAAGATCCTCCTA AAGCAGAAAGTCCAGGCACAGAACCCACAGCTAATTCAACAGTCACTGAGACGAATTCTACCCAACCAAA TCCTAAAGGGAGTCAGGGAGGAAATGACACCAGCCCCACAGGAAACAGTACCCCAGGACTAAACACTGGG AACAACCCTCCAGCTCAAAATGGGATTGGCCCACTCCCTGCAGTCAACGCTTCAGGCCAGGGAGGGCCAG GAAGTCAAATCCCATGGAGACCAAGTCAGCCAAATATTCGTGAAAATCATCCATATCCTAATATAAGAAA TTTTCCTTCAGGAAGACAGTGGTATTTCACTGGTACTGTCATGGGGCACAGACAGAATAGGCCTTTTTAC AGAAATCAACAAGTTCAAAGGGGTCCTCGGTGGAACTTCTTTGCTTGGGAACGTAAACAAGTAGCTCGTC CAGGAAATCCAGTTTATCACAAAGCTTACCCTCCTACTTCAAGAGGCAATTATCCCAATTATGCAGGAAA TCCAGCAAATCTCAGAAGAAAGCCTCAGGGGCCAAATAAACACCCTGTAGGAACTACTGTTGCCCCACTG GGTCCCAAACCTGGCCCTGTTGTTCGCAATGAAAAAATCCAAAATCCAAAGGAGAAGCCCCTGGGTCCAA AAGAACAAATAATAGTTCCTACAAAGAATCCAACCAGCCCCTGGAGAAACTCTCAACAGTATGAAGTTAA TAAATCAAATTATAAACTGCCTCACTCTGAGGGTTATATGCCAGTCCCAAATTTTAATTCTGTTGATCAA CATGAAAACTCCTATTACCCAAGAGGAGATTCCAGAAAAGTCCCAAATTCTGATGGACAAACCCAAAGCC AGAATTTGCCCAAAGGGATTGTTTTAGGGTCAAGAAGGATGCCATATGAATCAGAAACTAATCAGTCAGA ATTAAAGCACAGCTCATATCAGCCTGCTGTATACCCTGAGGAAATCCCTTCTCCTGCAAAAGAACATTTT CCTGCTGGAAGAAATACTTGGGACCACCAAGAAATCTCTCCACCTTTTAAGGAAGATCCAGGGAGGCAAG AAGAACATTTACCCCATCCTTCCCATGGTTCTAGAGGAAGTGTTTTCTACCCTGAATATAACCCATATGA TCCCAGGGAAAACTCACCATACCTTAGAGGCAATACATGGGATGAGAGAGATGATTCTCCCAATACTATG GGGCAAAAAGAAAGTCCACTCTACCCCATAAATACCCCAGACCAGAAGGAGATAGTCCCTTATAATGAAG AGGACCCAGTTGATCCAACTGGAGATGAAGTCTTTCCTGGACAAAATAGATGGGGTGAAGAGTTGAGCTT CAAAGGAGGCCCAACAGTTAGGCACTATGAAGGTGAACAATATACCTCAAATCAGCCAAAGGAATATCTT CCCTATTCTTTAGATAATCCATCAAAACCAAGGGAGGATTTTTATTACAGTGAATTTTACCCATGGAGCC CGGATGAGAATTTTCCATCATATAATACAGCTTCTACTATGCCACCACCTATAGAGAGCAGGGGCTACTA CGTTAATAATGCCGCTGGACCAGAAGAAAGCACTCTATTTCCTTCACGGAATTCCTGGGACCACAGGATA CAAGCCCAAGGGCAGAGAGAAAGAAGGCCGTATTTTAACAGAAATATCTGGGATCAGGCAACACATTTAC AAAAAGCCCCAGCTAGGCCACCAGACCAGAAAGGTAACCAGCCCTATTACAGTAACACCCCAGCTGGGCT TCAGAAAAATCCAATATGGCATGAAGGTGAGAATTTGAACTATGGCATGCAAATAACTAGGATGAATTCT CCAGAGAGAGAACATTCATCTTTCCCTAACTTCATCCCACCAAGTTACCCATCAGGTCAAAAAGAAGCAC ATTTATTTCACCTAAGCCAGAGAGGCTCTTGCTGTGCTGGTAGCTCCACAGGGCCCAAGGACAATCCACT AGCTCTACAAGACTACACTCCATCCTATGGTCTTGCACCTGGGGAGAACCAAGACACCAGTCCTCTGTAT ACAGACGGTAGTCATACCAAGCAGACAAGAGATATCATCTCCCCAACAAGCATCCTACCAGGCCAAAGAA ACAGCTCAGAGAAGAGGGAAAGCCAAAACCCTTTTAGAGATGATGTGTCCACGCTGAGGAGGAACACACC ATGTTCTATAAAGAATCAACTGGGCCAAAAGGAAATTATGCCCTTTCCTGAAGCCAGTTCCCTTCAATCA AAGAATACACCTTGTCTCAAAAATGATCTTGGAGGAGATGGGAACAACATTCTGGAACAAGTTTTTGAAG ACAACCAGCTCAATGAAAGAACTGTTGACCTTACTCCTGAGCAGCTTGTTATTGGTACACCTGATGAAGG CTCCAATCCAGAAGGCATCCAAAGTCAAGTCCAAGAAAATGAGAGTGAGAGGCAACAGCAAAGACCATCT AACATTCTGCATTTGCCATGCTTTGGCTCCAAATTAGCAAAGCATCACTCTTCCACCACCGGAACTCCAT CTAGCGATGGAAGGCAAAGCCCATTTGATGGGGATTCAATTACGCCTACTGAAAATCCTAACACATTGGT TGAGTTAGCTACTGAGGAACAATTTAAGAGTATAAATGTAGACCCACTTGATGCAGATGAACACAGTCCA TTTGAATTCCTTCAAAGAGGGACCAATGTACAGGACCAGGTACAAGACTGCTTACTACTTCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215089 representing NM_031889
Red=Cloning site Green=Tags(s) MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPH MAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLK QPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGG RPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTG NNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFY RNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPL GPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQ HENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHF PAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTM GQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYL PYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRI QAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNS PEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLY TDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQS KNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPS NILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSP FEFLQRGTNVQDQVQDCLLLQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031889 |
| ORF Size | 3426 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031889.3 |
| RefSeq Size | 5674 bp |
| RefSeq ORF | 3429 bp |
| Locus ID | 10117 |
| UniProt ID | Q9NRM1 |
| Protein Families | Secreted Protein |
| MW | 128.8 kDa |
| Gene Summary | Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C.[provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215089L3 | Lenti ORF clone of Human enamelin (ENAM), Myc-DDK-tagged |
CNY 13,300.00 |
|
| RC215089L4 | Lenti ORF clone of Human enamelin (ENAM), mGFP tagged |
CNY 13,300.00 |
|
| RG215089 | ENAM (tGFP-tagged) - Human enamelin (ENAM) |
CNY 12,540.00 |
|
| SC305355 | ENAM (untagged)-Human enamelin (ENAM) |
CNY 7,960.00 |
