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COPA (NM_004371) Human Tagged ORF Clone
CAT＃: RC214930
- TrueORF®
COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2
ORF Plasmid: tGFP
"NM_004371" in other vectors (4)
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CNY 12,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AILJK; alpha-COP; HEP-COP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214930 representing NM_004371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAACCAAATTCGAGACCAAGAGCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAAAGACCTTGGA TCCTGACTAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACTCTCATTGACAAGTT TGATGAACATGATGGTCCAGTGCGAGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGA GATGACTATAAGATTAAGGTTTGGAATTACAAGCTTCGGCGCTGTCTTTTCACATTGCTTGGGCACTTAG ATTATATTCGCACCACGTTTTTTCATCATGAATATCCCTGGATTCTGAGTGCCTCCGATGATCAGACCAT CCGAGTGTGGAACTGGCAATCTAGAACCTGTGTTTGTGTGTTAACAGGGCACAACCATTATGTGATGTGT GCTCAGTTCCACCCCACAGAAGACTTGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGCGTTTGGGATA TTTCTGGTCTGAGGAAAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGATGTGAGAGGAATAACTGGGGT TGATCTATTTGGAACTACAGATGCAGTGGTGAAGCATGTACTAGAGGGTCACGATCGTGGAGTAAACTGG GCTGCCTTCCACCCCACTATGCCCCTTATTGTATCTGGGGCAGATGATCGTCAAGTGAAGATCTGGCGCA TGAATGAATCAAAGGCATGGGAGGTTGATACCTGCCGGGGCCATTACAACAATGTATCTTGTGCCGTCTT CCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG CGGACTGGGGTTCAGACTTTCCGCAGAGACCATGATCGTTTCTGGGTCCTAGCTGCTCACCCTAACCTTA ACCTCTTTGCAGCAGGCCATGATGGTGGTATGATTGTGTTTAAGCTGGAACGGGAACGGCCAGCCTATGC TGTTCATGGCAATATGCTACACTATGTCAAGGACCGATTCTTACGACAGCTGGATTTCAACAGCTCCAAA GATGTAGCTGTGATGCAGTTGCGGAGTGGTTCCAAGTTTCCAGTATTCAATATGTCATACAATCCAGCAG AAAATGCAGTCCTGCTTTGTACAAGAGCTAGCAATCTAGAGAATAGTACCTATGACCTGTACACCATCCC TAAAGATGCTGACTCCCAGAATCCTGATGCGCCTGAAGGGAAACGATCCTCAGGCCTGACAGCCGTTTGG GTCGCTCGAAATCGGTTTGCTGTCCTAGATCGGATGCATTCGCTTCTGATCAAGAATCTGAAGAATGAGA TCACCAAAAAGGTACAGGTGCCCAACTGTGATGAGATCTTCTATGCTGGCACAGGCAATCTCCTGCTTCG AGATGCGGACTCTATCACACTCTTTGACGTACAGCAGAAGCGGACTCTGGCATCTGTGAAGATTTCTAAA GTGAAATACGTTATCTGGTCAGCAGACATGTCACATGTAGCACTACTAGCCAAACACGCCATTGTGATCT GTAACCGCAAACTGGATGCTTTATGTAACATTCATGAGAACATTCGTGTCAAGAGTGGGGCCTGGGATGA GAGTGGGGTATTTATCTATACCACAAGCAACCACATCAAATATGCTGTCACCACTGGGGACCACGGGATC ATTCGAACTCTGGATTTACCCATCTATGTCACACGGGTGAAGGGCAACAATGTATACTGCCTAGACAGGG AGTGTCGTCCCCGGGTACTCACCATTGATCCCACTGAGTTCAAATTCAAGCTGGCCCTGATCAACAGAAA ATATGATGAGGTACTGCACATGGTGAGGAATGCCAAACTAGTTGGCCAGTCTATTATTGCTTATCTCCAG AAGAAGGGCTATCCTGAAGTGGCACTGCATTTTGTCAAGGATGAGAAAACTCGCTTTAGTCTGGCACTGG AGTGTGGAAACATTGAGATTGCTCTGGAAGCAGCCAAAGCACTGGATGACAAGAACTGCTGGGAAAAGCT GGGAGAAGTGGCCCTGCTGCAGGGGAACCACCAGATTGTGGAAATGTGCTATCAGCGTACCAAAAACTTT GACAAACTTTCCTTCCTGTATCTTATCACTGGCAACTTAGAAAAACTTCGCAAGATGATGAAGATTGCTG AGATCAGAAAGGACATGAGTGGCCACTATCAGAATGCCCTATACCTGGGTGATGTGTCAGAGCGTGTGCG GATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTCACAGCTGCTACCCATGGCTTAGATGAAGAA GCTGAGAGCCTAAAGGAGACATTTGACCCAGAGAAGGAGACAATCCCAGACATTGACCCTAATGCCAAGC TGCTCCAGCCACCTGCACCTATCATGCCATTGGATACCAATTGGCCTTTATTGACTGTATCCAAAGGATT TTTTGAAGGCACCATTGCCAGCAAAGGGAAGGGAGGAGCACTGGCTGCTGACATTGACATTGACACTGTT GGTACAGAGGGCTGGGGAGAGGATGCAGAGCTGCAGTTGGATGAAGATGGGTTTGTGGAGGCTACAGAAG GTTTGGGGGATGATGCTCTTGGCAAGGGACAGGAAGAAGGAGGTGGCTGGGATGTAGAAGAAGATCTGGA GCTCCCTCCTGAGCTGGATATATCCCCTGGGGCAGCTGGTGGGGCTGAAGATGGTTTCTTTGTGCCCCCA ACCAAGGGAACAAGTCCAACTCAGATCTGGTGTAATAACTCTCAGCTTCCAGTTGATCACATCCTGGCAG GCTCTTTCGAAACAGCCATGCGGCTCCTTCATGACCAAGTAGGGGTAATCCAGTTTGGCCCCTACAAGCA ACTGTTCCTACAGACATACGCCCGAGGCCGCACAACCTATCAGGCTCTGCCCTGCCTACCCTCCATGTAT GGCTATCCTAATCGCAACTGGAAGGATGCAGGGCTGAAGAATGGTGTACCAGCTGTGGGCCTGAAGCTTA ATGACCTCATCCAACGGTTGCAGCTGTGCTACCAGCTCACCACAGTTGGCAAATTTGAGGAGGCTGTGGA AAAATTCCGTTCCATCCTTCTCAGTGTGCCACTTCTTGTTGTGGACAATAAACAAGAGATTGCAGAGGCC CAGCAGCTCATCACCATTTGCCGTGAGTACATTGTGGGTTTGTCCGTGGAGACAGAAAGGAAGAAGCTGC CCAAAGAGACTCTAGAACAGCAGAAGCGCATCTGTGAGATGGCAGCCTATTTCACCCACTCAAACCTGCA GCCTGTGCACATGATCCTGGTGCTGCGTACAGCCCTCAATCTGTTCTTCAAGCTCAAGAACTTCAAGACA GCTGCCACCTTTGCTCGGCGCCTACTAGAACTCGGGCCCAAGCCTGAGGTGGCCCAACAGACCCGAAAAA TCCTGTCTGCCTGTGAGAAGAATCCCACAGATGCCTACCAGCTCAATTATGACATGCACAACCCCTTTGA CATTTGTGCTGCATCATATCGGCCCATCTACCGTGGAAAGCCAGTAGAAAAGTGTCCACTCAGTGGGGCC TGCTATTCCCCTGAGTTCAAAGGTCAAATCTGCAGGGTCACCACAGTGACAGAGATTGGCAAAGATGTGA TTGGTTTAAGGATCAGTCCTCTGCAGTTTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214930 representing NM_004371
Red=Cloning site Green=Tags(s) MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGG DDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMC AQFHPTEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNW AAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSK RTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSK DVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVW VARNRFAVLDRMHSLLIKNLKNEITKKVQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISK VKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGI IRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQ KKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNF DKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLTAATHGLDEE AESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGTIASKGKGGALAADIDIDTV GTEGWGEDAELQLDEDGFVEATEGLGDDALGKGQEEGGGWDVEEDLELPPELDISPGAAGGAEDGFFVPP TKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMY GYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEA QQLITICREYIVGLSVETERKKLPKETLEQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKT AATFARRLLELGPKPEVAQQTRKILSACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGA CYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004371 |
| ORF Size | 3672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004371.4 |
| RefSeq Size | 5639 bp |
| RefSeq ORF | 3675 bp |
| Locus ID | 1314 |
| UniProt ID | P53621 |
| Domains | WD40, Coatomer_WDAD |
| MW | 138.3 kDa |
| Gene Summary | In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214930L3 | Lenti-ORF clone of COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2 |
CNY 14,160.00 |
|
| RC214930L4 | Lenti-ORF clone of COPA (mGFP-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2 |
CNY 14,160.00 |
|
| RG214930 | COPA (tGFP-tagged) - Human coatomer protein complex, subunit alpha (COPA), transcript variant 2 |
CNY 13,490.00 |
|
| SC126655 | COPA (untagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2 |
CNY 8,528.00 |
