GLT6D1 (NM_182974) Human Tagged ORF Clone

CAT#: RC214761

  • TrueORF®

GLT6D1 (Myc-DDK-tagged)-Human glycosyltransferase 6 domain containing 1 (GLT6D1)

ORF Plasmid: DDK tGFP



  "NM_182974" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal Anti-GLT6D1 Antibody
    • 50 ug

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GLTDC1; GT6M7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214761 representing NM_182974
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTCTAAAAGAATGCTGTTATTGGTTTTATTTGCTTTTTCACTGATGTTGGTTGAGCGTTATTTCA
GGAATCACCAAGTAGAAGAACTTCGGCTCTCAGACTGGTTTCATCCTAGAAAACGCCCTGATGTTATAAC
GAAAACAGACTGGCTCGCTCCTGTCCTATGGGAAGGGACTTTCGACAGGCGGGTCCTGGAAAAACATTAC
AGAAGGCGGAATATCACTGTGGGCCTGGCCGTCTTTGCTACTGGCAGGTTTGCAGAGGAGTACCTGAGGC
CGTTCCTACACTCCGCAAATAAGCACTTCATGACAGGCTACCGAGTGATCTTCTACATCATGGTGGACGC
CTTCTTCAAGCTGCCTGACATAGAGCCCAGTCCTCTTCGAACGTTCAAAGCATTTAAAGTGGGCACCGAG
AGGTGGTGGCTCGATGGCCCCCTGGTGCATGTGAAGAGCCTGGGTGAACACATCGCCAGTCACATCCAGG
ACGAGGTGGACTTCCTCTTCAGCATGGCTGCCAACCAGGTCTTCCAGAATGAGTTCGGGGTGGAGACCCT
GGGCCCGTTGGTGGCCCAGCTCCACGCCTGGTGGTATTTCAGAAACACCAAGAACTTCCCTTATGAGAGG
AGGCCGACCTCAGCAGCTTGCATCCCGTTTGGACAGGGAGATTTCTATTATGGCAACTTGATGGTTGGTG
GCACACCCCATAATATTTTAGACTTCATCAAAGAATATCTGAACGGAGTTATTCATGACATCAAAAATGG
ACTCAATAGCACTTATGAAAAGCACCTTAACAAATATTTTTACCTCAATAAACCCACTAAGCTGTTATCA
CCAGCATACAGCTGGGATCTTGCATTTTCTCCTCCTCCACAGATCCAATACGTCAAGGTCGCACATGATT
CCCAGAGGAAATTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214761 representing NM_182974
Red=Cloning site Green=Tags(s)

MNSKRMLLLVLFAFSLMLVERYFRNHQVEELRLSDWFHPRKRPDVITKTDWLAPVLWEGTFDRRVLEKHY
RRRNITVGLAVFATGRFAEEYLRPFLHSANKHFMTGYRVIFYIMVDAFFKLPDIEPSPLRTFKAFKVGTE
RWWLDGPLVHVKSLGEHIASHIQDEVDFLFSMAANQVFQNEFGVETLGPLVAQLHAWWYFRNTKNFPYER
RPTSAACIPFGQGDFYYGNLMVGGTPHNILDFIKEYLNGVIHDIKNGLNSTYEKHLNKYFYLNKPTKLLS
PAYSWDLAFSPPPQIQYVKVAHDSQRKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_182974
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182974.1, NP_892019.1
RefSeq Size 1526 bp
RefSeq ORF 831 bp
Locus ID 360203
UniProt ID Q7Z4J2
MW 32.4 kDa
Gene Summary The GT6 glycosyltransferases gene family, which includes the ABO blood group (ABO; MIM 110300) and GLT6D1, shows a complex evolution pattern, with multiple events of gain and loss in different mammal species. In humans, the ABO gene is considered the sole functional member, although the O allele is null and is fixed in certain populations (summary by Casals et al. (2009) [PubMed 19218399]).[supplied by OMIM, Jan 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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