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MC5R (NM_005913) Human Tagged ORF Clone
CAT＃: RC214760
- TrueORF®
MC5R (Myc-DDK-tagged)-Human melanocortin 5 receptor (MC5R)
ORF Plasmid: tGFP
"NM_005913" in other vectors (6)
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CNY 3,600.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214760 representing NM_005913
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCCTCATTTCACCTGCATTTCTTGGATCTCAACCTGAATGCCACAGAGGGCAACCTTTCAGGAC CCAATGTCAAAAACAAGTCTTCACCATGTGAAGACATGGGCATTGCTGTGGAGGTGTTTCTCACTCTGGG TGTCATCAGCCTCTTGGAGAACATCTTGGTCATAGGGGCCATAGTGAAGAACAAAAACCTGCACTCCCCC ATGTACTTCTTCGTGTGCAGCCTGGCAGTGGCGGACATGCTGGTGAGCATGTCCAGTGCCTGGGAGACCA TCACCATCTACCTACTCAACAACAAGCACCTAGTGATAGCAGACGCCTTTGTGCGCCACATTGACAATGT GTTTGACTCCATGATCTGCATTTCCGTGGTGGCATCCATGTGCAGCTTACTGGCCATTGCAGTGGATAGG TACGTCACCATCTTCTACGCCCTGCGCTACCACCACATCATGACGGCGAGGCGCTCAGGGGCCATCATCG CCGGCATCTGGGTTTTCTGCACGGGCTGCGGCATTGTCTTCATCCTGTACTCAGAATCCACCTACGTCAT CCTGTGCCTCATCTCCATGTTCTTCGCTATGCTGTTCCTCCTGGTGTCTCTGTACATACACATGTTCCTC CTGGCGCGGACTCACGTCAAGCGGATCGCGGCTCTGCCCGGGGCCAGCTCTGCGCGGCAGAGGACCAGCA TGCAGGGCGCGGTCACCGTCACCATGCTGCTGGGCGTGTTTACCGTGTGCTGGGCCCCGTTCTTCCTTCA TCTCACTTTAATGCTTTCTTGCCCTCAGAACCTCTACTGCTCTCGCTTCATGTCTCACTTCAATATGTAC CTCATACTCATCATGTGTAATTCCGTGATGGACCCTCTCATATATGCCTTCCGCAGCCAAGAGATGCGGA AGACCTTTAAGGAGATTATTTGCTGCCGTGGTTTCAGGATCGCCTGCAGCTTTCCCAGAAGGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214760 representing NM_005913
Red=Cloning site Green=Tags(s) MNSSFHLHFLDLNLNATEGNLSGPNVKNKSSPCEDMGIAVEVFLTLGVISLLENILVIGAIVKNKNLHSP MYFFVCSLAVADMLVSMSSAWETITIYLLNNKHLVIADAFVRHIDNVFDSMICISVVASMCSLLAIAVDR YVTIFYALRYHHIMTARRSGAIIAGIWVFCTGCGIVFILYSESTYVILCLISMFFAMLFLLVSLYIHMFL LARTHVKRIAALPGASSARQRTSMQGAVTVTMLLGVFTVCWAPFFLHLTLMLSCPQNLYCSRFMSHFNMY LILIMCNSVMDPLIYAFRSQEMRKTFKEIICCRGFRIACSFPRRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005913 |
| ORF Size | 975 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005913.3 |
| RefSeq Size | 978 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 4161 |
| UniProt ID | P33032 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 36.4 kDa |
| Gene Summary | This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation. [provided by RefSeq, Jun 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis
,Montero-Melendez, T;Nagano, A;Chelala, C;Filer, A;Buckley, CD;Perretti, M;,
Nat Commun
,PubMed ID 32029712
[MC5R]
|
|
Ligand Bias and Its Association With Pro-resolving Actions of Melanocortin Drugs
,Patruno, S;Garrido-Mesa, J;Romano, M;Perretti, M;Montero-Melendez, T;,
Front Pharmacol
,PubMed ID 30154720
[MC5R]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214760L1 | Lenti ORF clone of Human melanocortin 5 receptor (MC5R), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC214760L2 | Lenti ORF clone of Human melanocortin 5 receptor (MC5R), mGFP tagged |
CNY 5,890.00 |
|
| RC214760L3 | Lenti ORF clone of Human melanocortin 5 receptor (MC5R), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC214760L4 | Lenti ORF clone of Human melanocortin 5 receptor (MC5R), mGFP tagged |
CNY 6,000.00 |
|
| RG214760 | MC5R (tGFP-tagged) - Human melanocortin 5 receptor (MC5R) |
CNY 5,200.00 |
|
| SC116446 | MC5R (untagged)-Human melanocortin 5 receptor (MC5R) |
CNY 3,600.00 |
