United States
Germany
Japan
United Kingdom
China
PPM1E (NM_014906) Human Tagged ORF Clone
CAT＃: RC214622
- TrueORF®
PPM1E (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E)
ORF Plasmid: tGFP
"NM_014906" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,528.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214622 representing NM_014906
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGCGAGTTTCGCG GACCGTGCGGCGGCGGCGAGCCGGAGCCGGAACCCGAACCCGAACCCGAACCCGAACCCGAGTCCGAGCC CGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAGGCTTCGGTAGAGGAACCCGGGGAGGAGGCG GCCACGGTAGCCGCGACGGAGGAGGGGGACCAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTG AGGGTGAGGAGGAGGAGGAGGGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCC GCCGCCCCAGCTGCCGCCTTTGCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAGGAGGTG GAGGGCGAAAGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTTTTGGCTGCTG CTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTGCACATTATATCCCAAAGGAAAC GGATGGCACAGAAGGGACTGTGGAGATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACAC GAGATTTGCTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAA TCCATGCCATCAAAAACATGCGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCT CTTCAACCTAGAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCT GCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCATGATCCTGCTG AGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAG ATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGTGGCCTGGGTGGGTGATTCC CAGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATG AAAAGCAGAGAATTGAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCT GTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCC ACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTG ATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACAGCAGCATGGTTGCCCACAA ATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTATTGTGGTATTCCTGAGGGAC ATGAACAAAGCTGTAAATGTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAG ATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACC AGCCGACCTAGGCTATGATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAA ATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTGCCC AGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCAAATCTTATTAATGAGTTAAT GATGGAGAAAAAATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGTTTCCCACTGCTTTC AATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAA ATGAACAGTTCAAATCCCCGGGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAA GTGGCACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATA GGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGC AAAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGATATTCCATGCCCAGA TCTTCCTTGGAGCTATAAAATAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214622 representing NM_014906
Red=Cloning site Green=Tags(s) MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPESEPEPEPELVEAEAAEASVEEPGEEA ATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEV EGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLH EICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDA AIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDS QVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSAS TVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRD MNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQ INVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAF NLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKI GTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014906 |
| ORF Size | 2265 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014906.5 |
| RefSeq Size | 6545 bp |
| RefSeq ORF | 2268 bp |
| Locus ID | 22843 |
| UniProt ID | Q8WY54 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 84.4 kDa |
| Gene Summary | This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214622L1 | Lenti-ORF clone of PPM1E (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 7,700.00 |
|
| RC214622L2 | Lenti-ORF clone of PPM1E (mGFP-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 7,700.00 |
|
| RC214622L3 | Lenti-ORF clone of PPM1E (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 7,700.00 |
|
| RC214622L4 | Lenti-ORF clone of PPM1E (mGFP-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 7,700.00 |
|
| RG214622 | PPM1E (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 6,370.00 |
|
| SC128041 | PPM1E (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E) |
CNY 5,536.00 |
