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CHL1 (NM_006614) Human Tagged ORF Clone
CAT＃: RC214492
- TrueORF®
CHL1 (Myc-DDK-tagged)-Human cell adhesion molecule with homology to L1CAM (close homolog of L1) (CHL1)
ORF Plasmid: tGFP
"NM_006614" in other vectors (4)
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CNY 8,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CALL; L1CAM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214492 representing NM_006614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCTTTTACTTGGAAGAGGACTAATCGTATATCTAATGTTCCTCCTGTTAAAATTCTCAAAAG CAATTGAAATACCATCTTCAGTTCAACAGGTTCCAACAATCATAAAACAGTCAAAAGTCCAAGTTGCCTT TCCCTTCGATGAGTATTTTCAAATTGAATGTGAAGCTAAAGGAAATCCAGAACCAACATTTTCGTGGACT AAGGATGGCAACCCTTTTTATTTCACTGACCATCGGATAATTCCATCGAACAATTCAGGAACATTCAGGA TCCCAAACGAGGGGCACATATCTCACTTTCAAGGGAAATACCGCTGCTTTGCTTCAAATAAACTGGGAAT CGCTATGTCAGAAGAAATAGAATTTATAGTTCCAAGTGTTCCAAAATTCCCAAAAGAAAAAATTGACCCT CTTGAAGTGGAGGAGGGAGATCCAATTGTCCTCCCATGCAATCCTCCCAAAGGCCTCCCACCTTTACACA TTTATTGGATGAATATTGAATTAGAACACATCGAACAAGATGAAAGAGTATACATGAGCCAAAAGGGAGA TCTATACTTCGCAAACGTGGAAGAAAAGGACAGTCGCAATGACTACTGTTGCTTTGCTGCATTTCCAAGA TTAAGGACTATTGTACAGAAAATGCCAATGAAACTAACAGTTAACAGTTTAAAGCATGCTAATGACTCAA GTTCATCCACAGAAATTGGTTCCAAGGCAAATTCCATCAAGCAAAGAAAACCCAAACTGCTGTTGCCTCC CACTGAGAGTGGCAGTGAGTCTTCAATTACCATCCTCAAAGGGGAAATCTTGCTGCTTGAGTGTTTTGCT GAAGGCTTGCCAACTCCACAGGTTGATTGGAACAAAATTGGTGGTGACTTACCAAAGGGGAGAGAAACAA AAGAAAATTATGGCAAGACTTTGAAGATAGAGAATGTCTCCTACCAGGACAAAGGAAATTATCGCTGCAC AGCCAGCAATTTCTTGGGAACAGCCACTCACGATTTTCACGTTATAGTAGAAGAGCCTCCTCGCTGGACA AAGAAGCCTCAGAGTGCTGTGTATAGCACCGGAAGCAATGGCATCTTGTTATGTGAGGCTGAAGGAGAAC CTCAACCCACAATCAAGTGGAGAGTCAATGGCTCCCCAGTTGACAATCATCCATTTGCTGGTGATGTTGT CTTCCCCAGGGAAATCAGTTTTACCAACCTTCAACCAAATCATACTGCTGTGTACCAGTGTGAAGCCTCA AATGTCCATGGAACTATCCTTGCCAATGCCAATATTGATGTTGTGGATGTCCGTCCATTGATACAAACCA AAGATGGAGAAAATTACGCTACAGTGGTTGGGTACAGTGCTTTCTTACATTGCGAGTTCTTTGCTTCACC TGAGGCAGTCGTGTCCTGGCAGAAGGTGGAAGAAGTGAAACCCCTGGAGGGCAGGCGGTATCATATCTAT GAAAATGGCACATTGCAGATCAACAGAACCACCGAAGAAGATGCTGGGTCTTACTCATGTTGGGTAGAAA ATGCTATAGGAAAAACTGCAGTCACAGCCAATTTGGATATTAGAAATGCTACAAAACTTAGAGTTTCTCC TAAGAATCCTCGTATCCCCAAATTGCATATGCTTGAATTACATTGTGAAAGCAAATGTGACTCACATTTG AAACACAGTTTGAAGTTGTCCTGGAGTAAAGATGGAGAAGCCTTTGAAATTAATGGCACAGAAGATGGCA GGATAATTATTGATGGAGCTAATTTGACCATATCTAATGTAACTTTAGAGGACCAAGGTATTTACTGCTG TTCAGCTCATACTGCTCTAGACAGTGCTGCCGATATAACTCAAGTAACTGTTCTTGATGTTCCGGATCCA CCAGAAAACCTTCACTTGTCTGAAAGACAGAACAGGAGTGTTCGGCTGACCTGGGAAGCTGGAGCTGACC ACAACAGCAATATTAGCGAGTATATTGTTGAATTTGAAGGAAACAAAGAAGAGCCTGGAAGGTGGGAGGA ACTGACCAGAGTCCAAGGAAAGAAAACCACAGTTATCTTACCTTTGGCTCCATTTGTGAGATACCAGTTC AGGGTCATAGCCGTGAACGAAGTAGGGAGAAGTCAGCCTAGCCAGCCGTCAGACCATCATGAAACACCAC CAGCAGCTCCAGATAGGAATCCACAAAACATAAGGGTTCAAGCCTCTCAACCCAAGGAAATGATTATAAA GTGGGAGCCTTTGAAATCCATGGAGCAGAATGGACCAGGCCTAGAGTACAGAGTGACCTGGAAGCCACAG GGAGCCCCAGTGGAGTGGGAAGAAGAAACAGTCACAAACCACACATTGCGGGTGATGACGCCTGCTGTCT ATGCCCCTTATGATGTCAAGGTCCAGGCTATCAATCAACTAGGATCTGGGCCTGACCCTCAGTCAGTGAC TCTCTATTCTGGAGAAGACTATCCTGATACAGCTCCAGTGATCCATGGGGTGGACGTTATAAACAGTACA TTAGTTAAAGTTACCTGGTCAACAGTTCCAAAGGACAGAGTACATGGACGTCTGAAAGGCTATCAGATAA ATTGGTGGAAAACAAAAAGTCTGTTGGATGGAAGAACACATCCCAAAGAAGTGAACATTCTAAGATTTTC AGGACAAAGAAACTCTGGAATGGTTCCTTCCTTAGATGCCTTTAGTGAATTTCATTTAACAGTCTTAGCC TATAACTCTAAAGGAGCTGGTCCTGAAAGTGAGCCTTATATATTTCAAACACCAGAAGGAGTACCTGAAC AGCCAACTTTTCTAAAGGTCATCAAAGTTGATAAAGACACTGCCACTTTATCTTGGGGACTACCTAAGAA ATTAAATGGAAACTTAACTGGCTATCTTTTGCAATATCAGATAATAAATGACACCTACGAGATTGGAGAA TTAAATGATATTAACATTACAACTCCATCAAAGCCCAGCTGGCACCTCTCAAACCTGAATGCAACTACCA AGTACAAATTCTACTTGAGGGCTTGCACTTCACAGGGCTGTGGAAAACCGATCACGGAGGAAAGCTCCAC CTTAGGAGAAGGGAGTAAAGGTATCGGGAAGATATCAGGAGTAAATCTTACTCAAAAGACTCACCCAATA GAGGTATTTGAGCCGGGAGCTGAACATATAGTTCGCCTAATGACTAAGAATTGGGGCGATAATGATAGCA TTTTTCAAGATGTAATTGAGACAAGAGGGAGAGAATATGCTGGTTTATATGATGACATCTCCACTCAAGG CTGGTTTATTGGACTGATGTGTGCGATTGCTCTTCTCACACTACTATTATTAACTGTTTGCTTTGTGAAG AGGAATAGAGGTGGAAAGTACTCAGTTAAAGAAAAGGAAGATTTGCATCCAGACCCAGAAATTCAGTCAG TAAAAGATGAAACCTTTGGTGAATACAGTGACAGTGATGAAAAGCCTCTCAAAGGAAGCCTTCGGTCCCT TAATAGGGATATGCAGCCTACTGAAAGTGCTGACAGCTTAGTCGAATACGGAGAGGGAGACCATGGTCTC TTCAGTGAAGATGGATCATTTATTGGTGCCTACGCTGGATCTAAGGAGAAGGGATCTGTTGAAAGCAATG GAAGTTCTACAGCAACTTTTCCCCTTCGGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214492 representing NM_006614
Red=Cloning site Green=Tags(s) MEPLLLGRGLIVYLMFLLLKFSKAIEIPSSVQQVPTIIKQSKVQVAFPFDEYFQIECEAKGNPEPTFSWT KDGNPFYFTDHRIIPSNNSGTFRIPNEGHISHFQGKYRCFASNKLGIAMSEEIEFIVPSVPKFPKEKIDP LEVEEGDPIVLPCNPPKGLPPLHIYWMNIELEHIEQDERVYMSQKGDLYFANVEEKDSRNDYCCFAAFPR LRTIVQKMPMKLTVNSLKHANDSSSSTEIGSKANSIKQRKPKLLLPPTESGSESSITILKGEILLLECFA EGLPTPQVDWNKIGGDLPKGRETKENYGKTLKIENVSYQDKGNYRCTASNFLGTATHDFHVIVEEPPRWT KKPQSAVYSTGSNGILLCEAEGEPQPTIKWRVNGSPVDNHPFAGDVVFPREISFTNLQPNHTAVYQCEAS NVHGTILANANIDVVDVRPLIQTKDGENYATVVGYSAFLHCEFFASPEAVVSWQKVEEVKPLEGRRYHIY ENGTLQINRTTEEDAGSYSCWVENAIGKTAVTANLDIRNATKLRVSPKNPRIPKLHMLELHCESKCDSHL KHSLKLSWSKDGEAFEINGTEDGRIIIDGANLTISNVTLEDQGIYCCSAHTALDSAADITQVTVLDVPDP PENLHLSERQNRSVRLTWEAGADHNSNISEYIVEFEGNKEEPGRWEELTRVQGKKTTVILPLAPFVRYQF RVIAVNEVGRSQPSQPSDHHETPPAAPDRNPQNIRVQASQPKEMIIKWEPLKSMEQNGPGLEYRVTWKPQ GAPVEWEEETVTNHTLRVMTPAVYAPYDVKVQAINQLGSGPDPQSVTLYSGEDYPDTAPVIHGVDVINST LVKVTWSTVPKDRVHGRLKGYQINWWKTKSLLDGRTHPKEVNILRFSGQRNSGMVPSLDAFSEFHLTVLA YNSKGAGPESEPYIFQTPEGVPEQPTFLKVIKVDKDTATLSWGLPKKLNGNLTGYLLQYQIINDTYEIGE LNDINITTPSKPSWHLSNLNATTKYKFYLRACTSQGCGKPITEESSTLGEGSKGIGKISGVNLTQKTHPI EVFEPGAEHIVRLMTKNWGDNDSIFQDVIETRGREYAGLYDDISTQGWFIGLMCAIALLTLLLLTVCFVK RNRGGKYSVKEKEDLHPDPEIQSVKDETFGEYSDSDEKPLKGSLRSLNRDMQPTESADSLVEYGEGDHGL FSEDGSFIGAYAGSKEKGSVESNGSSTATFPLRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006614 |
| ORF Size | 3672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006614.4 |
| RefSeq Size | 7650 bp |
| RefSeq ORF | 3675 bp |
| Locus ID | 10752 |
| UniProt ID | O00533 |
| Domains | ig, IGc2, IG, FN3 |
| Protein Families | Transmembrane |
| MW | 136.7 kDa |
| Gene Summary | The protein encoded by this gene is a member of the L1 gene family of neural cell adhesion molecules. It is a neural recognition molecule that may be involved in signal transduction pathways. The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome. This protein may also play a role in the growth of certain cancers. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214492L3 | Lenti-ORF clone of CHL1 (Myc-DDK-tagged)-Human cell adhesion molecule with homology to L1CAM (close homolog of L1) (CHL1) |
CNY 14,160.00 |
|
| RC214492L4 | Lenti-ORF clone of CHL1 (mGFP-tagged)-Human cell adhesion molecule with homology to L1CAM (close homolog of L1) (CHL1) |
CNY 14,160.00 |
|
| RG214492 | CHL1 (tGFP-tagged) - Human cell adhesion molecule with homology to L1CAM (close homolog of L1) (CHL1) |
CNY 13,490.00 |
|
| SC115983 | CHL1 (untagged)-Human cell adhesion molecule with homology to L1CAM (close homolog of L1) (CHL1) |
CNY 8,528.00 |
