gamma Adducin (ADD3) (NM_019903) Human Tagged ORF Clone
CAT#: RC214407
- TrueORF®
ADD3 (Myc-DDK-tagged)-Human adducin 3 (gamma) (ADD3), transcript variant 2
ORF Plasmid: tGFP
"NM_019903" in other vectors (4)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ADDL; CPSQ3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214407 representing NM_019903
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGAT ATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACA AGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGAC TTGGAATGCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGA TTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCAC ACCTATCAATGACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTATATCTCAGTAA GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAAGCTACAGCCTC CAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGACCAGGGAAGTACCAATTTGAAAATTGACCAT ACAGGATTCAGTCCCCATGCTGCAATCTATTCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATA CCCTTGCAACAGCAGCTGTATCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCT GGGAGATGTTGCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAG AGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTGCAGGCCCTAGCAGGTGC AGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAGTATAAAGCTTTCACTTACACTGTAGCAGCG TCTGGTGGAGGAGGTGTGAATATGGGTTCCCATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGC TTATGAGGACTCTGGACAACTTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAA GCCTAGGCACAAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTGAACTCACCAA ATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACCAGTCCCCGAACCAAAATCAC GTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGAACACCTATCAAAATTGAAGATCCAAATCAG TTTGTTCCTTTAAACACAAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGAT ATGACTTGAAAACAGCAGGACCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTAC TATGCAATTTGAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGAAAACCATGAGCTGTTTT CCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAATGGCAAGGATGATATGCATGATGTTGA AGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAACCATAGAAAACATCGAGATTACTATT AAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTCCCCTTCAAAATCGCCATCCAAGAAAA AGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214407 representing NM_019903
Red=Cloning site Green=Tags(s) MSSDASQGVITTPPPPSMPHKERYFDRINENDPEYIRERNMSPDLRQDFNMMEQRKRVTQILQSPAFRED LECLIQEQMKKGHNPTGLLALQQIADYIMANSFSGFSSPPLSLGMVTPINDLPGADTSSYVKGEKLTRCK LASLYRLVDLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASNLVKVNIIGEVVDQGSTNLKIDH TGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLLLGDVAYYDYQGSLEEQEERIQLQK VLGPSCKVLVLRNHGVVALGETLEEAFHYIFNVQLACEIQVQALAGAGGVDNLHVLDFQKYKAFTYTVAA SGGGGVNMGSHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTV PLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQ FVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEG ELEEYKRTIERKQQGLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITI KSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019903 |
ORF Size | 2022 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019903.5 |
RefSeq Size | 4195 bp |
RefSeq ORF | 2025 bp |
Locus ID | 120 |
UniProt ID | Q9UEY8 |
Domains | Aldolase_II |
MW | 75.5 kDa |
Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008] |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214407L3 | Lenti-ORF clone of ADD3 (Myc-DDK-tagged)-Human adducin 3 (gamma) (ADD3), transcript variant 2 |
CNY 7,700.00 |
|
RC214407L4 | Lenti-ORF clone of ADD3 (mGFP-tagged)-Human adducin 3 (gamma) (ADD3), transcript variant 2 |
CNY 7,700.00 |
|
RG214407 | ADD3 (tGFP-tagged) - Human adducin 3 (gamma) (ADD3), transcript variant 2 |
CNY 6,370.00 |
|
SC127707 | ADD3 (untagged)-Human adducin 3 (gamma) (ADD3), transcript variant 2 |
CNY 13,110.00 |
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