PDE8B (NM_001029854) Human Tagged ORF Clone
CAT#: RC214347
- TrueORF®
PDE8B (Myc-DDK-tagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 2
ORF Plasmid: tGFP
"NM_001029854" in other vectors (4)
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CNY 8,840.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ADSD; PPNAD3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214347 representing NM_001029854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGGACTCGGACGAGTCCA GCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGAC CGACGCCGCCGACGCCATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCC CGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCCGCCGCGACCACCAGCAGGGGCCGGA GGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTC TGCGGAGGTGCGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAG GAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAATATTGCTCGGA CTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGACAAAC TCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCACACGGTGATC CTCGCAGTGGTTTCGCGAGTATCGGATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCT TCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACATGG GGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAA GCCATAGAAATAACAAGCGATGACCACGTGATTCAGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAAT CCGGGGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATTT TGTCTCGCTCAAGAAACTGTGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTCA GGAGACAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTGA AATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCCATGGCGAGGAT CCACTCCATGACCATCGAGGCTCCCATCACAAAGGTTATAAATATAATCAATGCAGCCCAAGAAAACAGC CCAGTCACAGTAGCGGAAGCCTTGGACAGAGTTCTAGAGATTTTACGGACCACAGAACTGTACTCCCCTC AGCTGGGTACCAAAGATGAAGATCCCCACACCAGTGATCTTGTTGGAGGCCTGATGACTGACGGCTTGAG AAGACTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCA ATAACCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAACA TCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTCTTCTCTCGGTT TGGAGTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGATCGAAGCCAAC TACCACTCTTCCAATGCCTACCACAACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTC TTGGAAAGGAAAGAGTAAAGGGAAGCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGT CCATGACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGCTTGCTGTGCTC TACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACCA AATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATTATTGACATGGT TTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGTTTGTGAACAGCATCAACAAGCCAATG GCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCC TGATCAAACGCATGATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGA ATGGGCTGGGAGGATCTCTGAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTACCTGTG GTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACTACTTCA TAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACATTTGGCTGACAA CTACAAACACTGGAAGACACTAGATGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214347 representing NM_001029854
Red=Cloning site Green=Tags(s) MGCAPSIHVSQSGVIYCRDSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADAIPPSRASGPPSVARVRRA RTELGSGSSAGSAAPAATTSRGRRRHCCSSAEAETQTCYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAK EDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHTVI LAVVSRVSDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHE AIEITSDDHVIQEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDNNKQIHKIHRDS GDNSQTEPHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENS PVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMP ITINDVPPCISQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCSETTLRAWFQVIEAN YHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVL YNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPM AAEIEGSDCECNPAGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPV VMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001029854 |
ORF Size | 2514 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001029854.4 |
RefSeq Size | 4264 bp |
RefSeq ORF | 2517 bp |
Locus ID | 8622 |
UniProt ID | O95263 |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
MW | 93.7 kDa |
Gene Summary | The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214347L3 | Lenti-ORF clone of PDE8B (Myc-DDK-tagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 2 |
CNY 10,740.00 |
|
RC214347L4 | Lenti-ORF clone of PDE8B (mGFP-tagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 2 |
CNY 10,740.00 |
|
RG214347 | PDE8B (tGFP-tagged) - Human phosphodiesterase 8B (PDE8B), transcript variant 2 |
CNY 9,690.00 |
|
SC302441 | PDE8B (untagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 2 |
CNY 16,250.00 |