Spt6 (SUPT6H) (NM_003170) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	emb-5; SPT6; SPT6H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214306 representing NM_003170 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGATTTTGTGGAAAGCGAGGCTGAGGAGTCAGAGGAAGAATACAATGATGAAGGCGAGGTGGTAC CCCGAGTCACCAAGAAATTTGTGGAAGAGGAGGATGATGATGAGGAGGAGGAGGAGGAGAACCTAGATGA TCAGGATGAGCAAGGCAACTTGAAAGGCTTTATCAATGACGATGATGATGAAGATGAAGGGGAGGAGGAT GAGGGCAGTGACTCTGGTGATTCAGAAGATGATGTTGGCCACAAGAAGAGAAAACGCACCTCTTTTGATG ACCGCCTGGAGGATGATGATTTTGACCTCATTGAGGAGAATTTGGGTGTCAAAGTCAAAAGAGGACAAAA GTACCGGCGTGTCAAAAAAATGTCAGATGACGAGGACGATGACGAGGAGGAATATGGCAAGGAGGAACAT GAAAAAGAAGCTATTGCGGAAGAAATCTTCCAGGATGGGGAAGGGGAAGAAGGGCAGGAGGCCATGGAGG CCCCCATGGCTCCTCCAGAGGAGGAGGAAGAAGATGATGAGGAGTCAGATATTGACGACTTCATTGTGGA TGATGATGGACAGCCTCTGAAAAAACCTAAGTGGCGGAAAAAGCTTCCTGGATACACAGACGCGGCCCTG CAAGAAGCCCAGGAAATCTTCGGTGTGGACTTTGACTATGATGAATTTGAGAAATACAATGAGTATGATG AAGAACTGGAGGAAGAGTATGAGTATGAGGATGATGAGGCTGAGGGTGAAATCCGAGTGCGCCCCAAGAA GACCACCAAGAAGCGTGTGAGCCGTAGGAGCATCTTTGAAATGTATGAGCCCAGTGAGCTAGAAAGCAGC CACCTCACAGATCAGGACAATGAAATCCGAGCCACTGACCTGCCTGAGAGGTTCCAGCTCCGCTCCATCC CAGTCAAGGGGGCTGAAGATGATGAACTAGAAGAAGAAGCTGACTGGATCTACAGGAATGCTTTTGCCAC ACCAACCATTTCTCTCCAGGAAAGCTGTGATTACCTAGACCGAGGGCAGCCAGCCAGCAGCTTCAGTCGG AAAGGGCCCAGCACAATTCAGAAGATCAAAGAGGCCCTGGGCTTCATGCGAAATCAGCATTTTGAGGTGC CTTTTATTGCCTTCTATCGAAAGGAGTATGTGGAGCCTGAGTTGCACATCAATGACCTATGGAGAGTCTG GCAGTGGGATGAAAAGTGGACCCAGCTGCGGATCCGTAAAGAGAACCTAACACGGCTGTTTGAGAAGATG CAGGCTTATCAGTATGAACAGATCTCTGCTGACCCTGACAAACCTCTTGCTGATGGCATCCGGGCTCTGG ACACCACTGACATGGAGAGGCTCAAGGATGTCCAATCAATGGATGAGCTGAAAGATGTCTACAACCATTT TCTTCTTTATTATGGCCGAGACATCCCTAAGATGCAGAACGCCGCCAAAGCTAGCCGCAAGAAGCTGAAG CGTGTCAGGGAAGAGGGAGATGAAGAAGGTGAAGGTGACGAGGCAGAAGATGAGGAGCAGAGGGGGCCTG AGCTCAAGCAAGCCTCTCGCCGAGACATGTACACCATCTGCCAGAGTGCTGGGCTAGATGGCCTGGCCAA AAAGTTTGGGCTTACTCCCGAGCAGTTTGGGGAGAACCTGCGGGATAGCTACCAGCGGCACGAGACAGAG CAGTTTCCCGCGGAGCCCTTGGAGCTGGCCAAGGATTACGTTTGCAGCCAGTTCCCTACTCCAGAAGCTG TGCTAGAAGGCGCCCGCTACATGGTAGCCCTGCAGATTGCCCGTGAGCCCCTTGTCCGGCAGGTGCTGAG GCAAACCTTCCAAGAGAGAGCCAAGTTAAATATAACCCCCACCAAGAAAGGTAGAAAGGATGTGGATGAG GCCCACTATGCCTATTCCTTCAAGTATTTAAAGAACAAGCCTGTTAAGGAACTGAGAGATGACCAGTTTC TCAAGATATGCCTGGCTGAAGACGAAGGGCTCCTCACCACTGACATCAGCATAGATTTGAAGGGAGTGGA AGGCTATGGCAACGACCAGACATATTTTGAGGAGATAAAACAGTTTTACTACCGAGATGAGTTCAGCCAC CAGGTGCAGGAGTGGAACCGGCAGCGCACCATGGCCATCGAACGGGCTTTACAGCAGTTCCTCTATGTGC AGATGGCCAAAGAACTCAAGAACAAGCTGCTGGCTGAAGCCAAGGAATATGTCATAAAGGCCTGTAGTCG AAAGCTCTACAATTGGTTGAGAGTGGCACCCTACCGACCAGATCAGCAGGTGGAAGAAGATGACGACTTT ATGGACGAGAACCAAGGGAAGGGCATTCGAGTCCTCGGCATTGCTTTCTCCTCTGCCAGAGATCACCCTG TGTTCTGCGCCCTGGTCAATGGTGAAGGAGAAGTGACAGACTTCCTTCGACTGCCCCATTTTACCAAACG GCGAACTGCATGGAGAGAGGAAGAGCGGGAAAAGAAGGCTCAAGACATTGAAACGCTAAAGAAATTTCTC CTGAATAAGAAGCCTCATGTAGTGACAGTTGCAGGAGAGAACAGGGACGCCCAGATGTTGATTGAAGATG TGAAGCGCATTGTACATGAGCTGGACCAGGGCCAGCAGCTGTCATCTATTGGGGTAGAGCTGGTTGACAA CGAGTTGGCCATTCTCTATATGAACAGCAAGAAGTCAGAGGCAGAGTTCCGGGATTATCCTCCAGTGCTG AGACAGGCCGTCTCCCTGGCCCGGCGCATCCAGGACCCTCTGATTGAATTTGCCCAGGTGTGCAGTTCCG ATGAAGACATCCTGTGTCTCAAGTTTCACCCCTTGCAGGAGCATGTGGTGAAAGAGGAGCTGCTCAACGC CTTGTACTGTGAATTTATCAACCGAGTCAATGAGGTCGGGGTCGATGTCAACCGTGCCATTGCCCACCCT TACAGCCAGGCCTTGATCCAGTATGTTTGTGGCCTGGGACCTCGGAAAGGGACCCACCTCCTGAAGATCC TGAAGCAGAACAACACCCGGCTCGAGAGCCGGACCCAGCTGGTCACCATGTGCCACATGGGTCCCAAAGT CTTCATGAATTGTGCTGGCTTCCTCAAGATCGACACGGCCTCCCTGGGGGACAGCACTGACTCATATATT GAAGTCCTTGATGGTTCCCGTGTCCACCCTGAGACTTATGAGTGGGCTAGGAAGATGGCAGTGGATGCCC TGGAATACGATGAATCAGCCGAGGATGCCAATCCTGCAGGAGCCCTTGAAGAAATCTTGGAAAACCCAGA GCGACTGAAAGACCTGGACCTTGATGCCTTTGCAGAAGAGCTGGAGAGGCAGGGCTATGGTGACAAACAC ATCACACTCTATGACATCCGGGCAGAGCTGAGCTGTCGATATAAGGACCTCCGGACAGCCTACCGCTCTC CCAACACAGAGGAGATCTTCAATATGTTAACCAAAGAAACACCAGAGACCTTCTACATTGGAAAGCTCAT CATCTGCAATGTCACTGGCATTGCCCACAGGCGTCCCCAGGGTGAGAGCTATGACCAGGCGATCCGCAAT GATGAGACAGGGCTGTGGCAGTGCCCCTTCTGTCAGCAGGACAATTTCCCTGAACTAAGCGAGGTGTGGA ACCACTTTGACAGCGGTTCGTGCCCAGGCCAGGCCATCGGTGTCAAAACACGGCTAGACAATGGTGTCAC CGGCTTCATCCCCACCAAATTCCTCAGTGACAAAGTGGTAAAGCGGCCAGAAGAACGAGTGAAGGTGGGA ATGACTGTTCACTGCCGCATCATGAAGATTGACATTGAGAAGTTCAGTGCAGACCTGACCTGCCGCACCT CAGACCTCATGGACAGGAACAATGAGTGGAAGCTGCCCAAAGACACCTACTATGACTTTGATGCTGAAGC TGCAGACCACAAGCAGGAGGAGGACATGAAGCGGAAGCAGCAGCGGACCACATACATCAAGAGAGTGATC GCACACCCATCCTTCCATAATATCAATTTCAAGCAAGCAGAAAAGATGATGGAGACCATGGACCAGGGTG ATGTGATTATCCGACCAAGCAGCAAGGGCGAGAACCACCTGACAGTGACCTGGAAAGTCAGTGATGGCAT CTACCAGCATGTGGATGTGCGGGAGGAGGGCAAGGAAAATGCCTTCAGCCTGGGAGCCACTCTGTGGATC AACAGTGAGGAATTCGAAGATTTGGATGAGATTGTTGCTCGCTATGTCCAGCCCATGGCATCCTTTGCCC GGGACCTTCTGAATCACAAGTATTATCAGGACTGCAGCGGTGGGGACCGCAAGAAATTAGAGGAGCTGCT CATCAAAACTAAGAAGGAGAAGCCCACCTTCATCCCTTATTTCATCTGTGCCTGCAAGGAACTGCCCGGC AAGTTCCTACTGGGATACCAGCCCCGGGGTAAACCCAGGATAGAATATGTAACGGTGACTCCAGAGGGAT TCCGGTACCGGGGCCAGATCTTCCCAACCGTGAATGGACTGTTTAGATGGTTTAAGGATCACTACCAGGA TCCTGTACCAGGCATCACCCCTAGCAGCAGCAGCAGGACCCGGACACCTGCCTCTATCAATGCTACCCCA GCCAACATCAACCTTGCAGATCTGACACGGGCTGTGAATGCCCTGCCTCAGAACATGACTTCACAGATGT TCAGTGCCATTGCTGCGGTGACAGGCCAAGGACAGAACCCTAATGCCACCCCAGCCCAGTGGGCCTCCAG CCAGTACGGCTATGGCGGCAGTGGAGGCGGCAGCAGTGCTTACCACGTATTCCCAACGCCAGCCCAGCAG CCAGTGGCCACACCACTAATGACCCCTAGCTACTCCTACACGACCCCAAGCCAGCCCATCACCACCCCTC AGTACCACCAGCTCCAGGCCAGCACCACCCCACAGTCGGCCCAGGCCCAGCCCCAGCCCTCTTCCAGCTC CCGGCAACGGCAGCAGCAGCCAAAGTCCAACAGCCATGCAGCCATCGACTGGGGAAAAATGGCGGAGCAG TGGCTGCAGGAAAAGGAGGCAGAACGGCGGAAACAGAAGCAGCGGCTGACACCTCGGCCCTCCCCCAGCC CCATGATCGAAAGCACCCCCATGTCCATTGCTGGCGATGCCACCCCACTCCTGGACGAGATGGATCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214306 representing NM_003170 Red=Cloning site Green=Tags(s) MSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEED EGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEH EKEAIAEEIFQDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAAL QEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESS HLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSR KGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKM QAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLK RVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETE QFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDE AHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSH QVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDF MDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFL LNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVL RQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHP YSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYI EVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKH ITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRN DETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVG MTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVI AHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWI NSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPG KFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATP ANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQ PVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQ WLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_003170
ORF Size	5178 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003170.5
RefSeq Size	5896 bp
RefSeq ORF	5181 bp
Locus ID	6830
UniProt ID	Q7KZ85
Domains	SH2, S1, YqgFc
Protein Families	Transcription Factors
MW	199.07 kDa
Gene Summary	Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.[UniProtKB/Swiss-Prot Function]

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