SLC36A1 (NM_078483) Human Tagged ORF Clone

CAT#: RC214295

SLC36A1 (Myc-DDK-tagged)-Human solute carrier family 36 (proton/amino acid symporter), member 1 (SLC36A1)

ORF Plasmid: DDK tGFP



  "NM_078483" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal SLC36A1 Antibody (N-term)
    • 400 ul

CNY 6,160.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms Dct1; LYAAT1; PAT1; TRAMD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214295 representing NM_078483
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACGCAGAGACTTCGGAATGAAGACTACCACGACTACAGCTCCACGGACGTGAGCCCTGAGGAGA
GCCCGTCGGAAGGCCTCAACAACCTCTCCTCCCCGGGCTCCTACCAGCGCTTTGGTCAAAGCAATAGCAC
AACATGGTTCCAGACCTTGATCCACCTGTTAAAAGGCAACATTGGCACAGGACTCCTGGGACTCCCTCTG
GCGGTGAAAAATGCAGGCATCGTGATGGGTCCCATCAGCCTGCTGATCATAGGCATCGTGGCCGTGCACT
GCATGGGTATCCTGGTGAAATGTGCTCACCACTTCTGCCGCAGGCTGAATAAATCCTTTGTGGATTATGG
TGATACTGTGATGTATGGACTAGAATCCAGCCCCTGCTCCTGGCTCCGGAACCACGCACACTGGGGAAGA
CGTGTTGTGGACTTCTTCCTGATTGTCACCCAGCTGGGATTCTGCTGTGTCTATTTTGTGTTTCTGGCTG
ACAACTTTAAACAGGTGATAGAAGCGGCCAATGGGACCACCAATAACTGCCACAACAATGAGACGGTGAT
TCTGACGCCTACCATGGACTCGCGACTCTACATGCTCTCCTTCCTGCCCTTCCTGGTGCTGCTGGTTTTC
ATCAGGAACCTCCGAGCCCTGTCCATCTTCTCCCTGTTGGCCAACATCACCATGCTGGTCAGCTTGGTCA
TGATCTACCAGTTCATTGTTCAGAGGATCCCAGACCCCAGCCACCTCCCCTTGGTGGCCCCTTGGAAGAC
CTACCCTCTCTTCTTTGGCACAGCGATTTTTTCATTTGAAGGCATTGGAATGGTTCTGCCCCTGGAAAAC
AAAATGAAGGATCCTCGGAAGTTCCCACTCATCCTGTACCTGGGCATGGTCATCGTCACCATCCTCTACA
TCAGCCTGGGGTGTCTGGGGTACCTGCAATTTGGAGCTAATATCCAAGGCAGCATAACCCTCAACCTGCC
CAACTGCTGGTTGTACCAGTCAGTTAAGCTGCTGTACTCCATCGGGATCTTTTTCACCTACGCACTCCAG
TTCTACGTCCCGGCTGAGATCATCATCCCCTTCTTTGTGTCCCGAGCGCCCGAGCACTGTGAGTTAGTGG
TGGACCTGTTTGTGCGCACAGTGCTGGTCTGCCTGACATGCATCTTGGCCATCCTCATCCCCCGCCTGGA
CCTGGTCATCTCCCTGGTGGGCTCCGTGAGCAGCAGCGCCCTGGCCCTCATCATCCCACCGCTCCTGGAG
GTCACCACCTTCTACTCAGAGGGCATGAGCCCCCTCACCATCTTTAAGGACGCCCTGATCAGCATCCTGG
GCTTCGTGGGCTTTGTGGTGGGGACCTATGAGGCTCTCTATGAGCTGATCCAGCCAAGCAATGCTCCCAT
CTTCATCAATTCCACCTGTGCCTTCATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214295 representing NM_078483
Red=Cloning site Green=Tags(s)

MSTQRLRNEDYHDYSSTDVSPEESPSEGLNNLSSPGSYQRFGQSNSTTWFQTLIHLLKGNIGTGLLGLPL
AVKNAGIVMGPISLLIIGIVAVHCMGILVKCAHHFCRRLNKSFVDYGDTVMYGLESSPCSWLRNHAHWGR
RVVDFFLIVTQLGFCCVYFVFLADNFKQVIEAANGTTNNCHNNETVILTPTMDSRLYMLSFLPFLVLLVF
IRNLRALSIFSLLANITMLVSLVMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFSFEGIGMVLPLEN
KMKDPRKFPLILYLGMVIVTILYISLGCLGYLQFGANIQGSITLNLPNCWLYQSVKLLYSIGIFFTYALQ
FYVPAEIIIPFFVSRAPEHCELVVDLFVRTVLVCLTCILAILIPRLDLVISLVGSVSSSALALIIPPLLE
VTTFYSEGMSPLTIFKDALISILGFVGFVVGTYEALYELIQPSNAPIFINSTCAFI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_078483
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_078483.4
RefSeq Size 5821 bp
RefSeq ORF 1431 bp
Locus ID 206358
UniProt ID Q7Z2H8
Domains Aa_trans
Protein Families Transmembrane
MW 52.9 kDa
Gene Summary This gene encodes a member of the eukaryote-specific amino acid/auxin permease (AAAP) 1 transporter family. The encoded protein functions as a proton-dependent, small amino acid transporter. This gene is clustered with related family members on chromosome 5q33.1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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