United States
Germany
Japan
United Kingdom
China
TTC10 (IFT88) (NM_006531) Human Tagged ORF Clone
CAT＃: RC214282
- TrueORF®
IFT88 (Myc-DDK-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2
ORF Plasmid: tGFP
"NM_006531" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D13S1056E; DAF19; hTg737; TG737; TTC10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214282 representing NM_006531
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGATCTTTATTCCGGCTATAATGACTACA ATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTTTCAGCAAGCTGTGAGGACTAGTCATGG CAGAAGACCTCCAATAACTGCTAAAATATCAAGCACGGCAGTTACTAGACCTATAGCTACTGGATATGGG TCCAAGACATCTCTGGCATCATCAATAGGAAGACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGAC CCATGACAGCAGTGAGAGCAGCTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCCCTTAG TCAGTCAAGGGGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAAGATAGCCCAGAGGAAAAAATAAAGCAA TTAGAGAAGGAAGTAAATGAGTTGGTAGAAGAAAGCTGTATTGCCAATAGTTGTGGAGACTTAAAATTGG CCTTAGAAAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCTGGTGAGACAGCGAGAACAAGTTACAAC TCCAGAAAATATCAATTTGGATTTAACTTACTCAGTTCTTTTCAATTTGGCCAGTCAGTATTCAGTTAAT GAAATGTATGCCGAAGCACTTAACACTTATCAAGTTATAGTCAAAAATAAGATGTTTAGCAATGCAGGAA TATTGAAAATGAATATGGGAAATATCTATTTAAAGCAAAGAAATTATTCCAAAGCCATTAAATTCTACCG AATGGCATTAGACCAAGTTCCAAGTGTCAATAAGCAAATGAGGATTAAAATAATGCAGAATATTGGAGTT ACATTTATTCAGGCTGGTCAGTATTCAGATGCTATTAATTCATATGAGCACATAATGAGCATGGCACCAA ATCTGAAGGCAGGCTACAACCTAACTATCTGTTATTTTGCTATTGGAGACCGAGAAAAAATGAAGAAGGC ATTCCAAAAATTGATTACTGTTCCATTAGAAATTGATGAAGATAAATATATTTCACCAAGTGATGATCCT CATACTAACTTAGTAACTGAAGCTATAAAAAATGATCACCTCAGGCAAATGGAACGTGAAAGGAAAGCCA TGGCAGAAAAATATATTATGACATCTGCAAAACTCATTGCTCCTGTAATTGAAACATCTTTTGCTGCAGG TTATGATTGGTGCGTGGAAGTGGTGAAAGCTTCTCAATATGTAGAGCTAGCCAATGATCTGGAAATAAAC AAAGCAGTTACATACTTGAGACAAAAAGACTATAACCAAGCTGTAGAGATCTTAAAAGTGTTGGAAAAAA AGGACAGTAGAGTGAAAAGTGCAGCTGCAACCAATCTCTCAGCCCTGTATTATATGGGAAAGGATTTTGC ACAAGCCAGCAGCTATGCAGATATAGCTGTGAACTCTGATAGATATAATCCAGCAGCTCTTACTAATAAA GGGAATACAGTTTTTGCAAATGGTGATTATGAGAAGGCCGCTGAATTCTATAAAGAGGCTCTAAGAAATG ATTCTTCTTGTACTGAAGCACTTTATAATATTGGCCTTACCTATGAGAAACTAAATCGGCTAGATGAGGC TTTGGACTGTTTCCTGAAACTTCACGCAATCCTACGAAACAGTGCCGAAGTTCTTTACCAGATAGCAAAT ATATATGAATTAATGGAAAATCCCAGTCAAGCTATTGAATGGCTAATGCAGGTGGTCAGTGTTATTCCAA CCGATCCTCAAGTTTTATCTAAGCTAGGAGAATTATATGATCGTGAAGGAGATAAATCTCAAGCATTTCA ATATTACTATGAGTCATATAGGTATTTTCCTTGTAATATTGAAGTCATTGAGTGGCTTGGAGCCTATTAC ATTGACACCCAATTTTGGGAAAAAGCTATTCAGTACTTTGAAAGAGCTTCTCTTATACAGCCTACACAAG TGAAATGGCAGCTGATGGTAGCTAGTTGTTTCAGAAGAAGTGGTAACTACCAAAAAGCATTAGATACTTA CAAAGATACTCACAGAAAATTTCCAGAAAATGTCGAATGTCTGCGTTTCTTAGTTCGTCTCTGCACAGAT CTTGGATTAAAAGATGCTCAAGAATATGCCAGAAAACTGAAGAGGTTGGAAAAAATGAAAGAAATAAGGG AACAGCGCATAAAGTCAGGCAGAGATGGCAGTGGGGGCTCCCGTGGCAAAAGAGAAGGAAGTGCTAGCGG TGATAGTGGCCAGAACTATAGTGCCAGTAGTAAAGGTGAACGACTAAGTGCCAGACTCAGAGCTTTACCT GGGACAAATGAACCTTATGAAAGTAGCAGTAACAAAGAAATAGATGCCTCCTATGTGGACCCACTTGGCC CTCAAATAGAACGACCAAAAACTGCAGCCAAGAAAAGGATCGATGAGGATGATTTTGCTGATGAAGAATT AGGAGATGATTTGCTTCCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214282 representing NM_006531
Red=Cloning site Green=Tags(s) MMQNVHLAPETDEDDLYSGYNDYNPIYDIEELENDAAFQQAVRTSHGRRPPITAKISSTAVTRPIATGYG SKTSLASSIGRPMTGAIQDGVTRPMTAVRAAGFTKAALRGSAFDPLSQSRGPASPLEAKKKDSPEEKIKQ LEKEVNELVEESCIANSCGDLKLALEKAKDAGRKERVLVRQREQVTTPENINLDLTYSVLFNLASQYSVN EMYAEALNTYQVIVKNKMFSNAGILKMNMGNIYLKQRNYSKAIKFYRMALDQVPSVNKQMRIKIMQNIGV TFIQAGQYSDAINSYEHIMSMAPNLKAGYNLTICYFAIGDREKMKKAFQKLITVPLEIDEDKYISPSDDP HTNLVTEAIKNDHLRQMERERKAMAEKYIMTSAKLIAPVIETSFAAGYDWCVEVVKASQYVELANDLEIN KAVTYLRQKDYNQAVEILKVLEKKDSRVKSAAATNLSALYYMGKDFAQASSYADIAVNSDRYNPAALTNK GNTVFANGDYEKAAEFYKEALRNDSSCTEALYNIGLTYEKLNRLDEALDCFLKLHAILRNSAEVLYQIAN IYELMENPSQAIEWLMQVVSVIPTDPQVLSKLGELYDREGDKSQAFQYYYESYRYFPCNIEVIEWLGAYY IDTQFWEKAIQYFERASLIQPTQVKWQLMVASCFRRSGNYQKALDTYKDTHRKFPENVECLRFLVRLCTD LGLKDAQEYARKLKRLEKMKEIREQRIKSGRDGSGGSRGKREGSASGDSGQNYSASSKGERLSARLRALP GTNEPYESSSNKEIDASYVDPLGPQIERPKTAAKKRIDEDDFADEELGDDLLPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006531 |
| ORF Size | 2472 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006531.5 |
| RefSeq Size | 2872 bp |
| RefSeq ORF | 2475 bp |
| Locus ID | 8100 |
| UniProt ID | Q13099 |
| Domains | TPR |
| MW | 93 kDa |
| Gene Summary | This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214282L3 | Lenti-ORF clone of IFT88 (Myc-DDK-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 7,700.00 |
|
| RC214282L4 | Lenti-ORF clone of IFT88 (mGFP-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 7,700.00 |
|
| RG214282 | IFT88 (tGFP-tagged) - Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 6,370.00 |
|
| SC109922 | IFT88 (untagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 6,040.00 |
