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COPA (NM_001098398) Human Tagged ORF Clone
CAT＃: RC214199
COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1
ORF Plasmid: tGFP
"NM_001098398" in other vectors (6)
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CNY 12,880.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AILJK; alpha-COP; HEP-COP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214199 representing NM_001098398
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAACCAAATTCGAGACCAAGAGCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAAAGACCTTGGA TCCTGACTAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACTCTCATTGACAAGTT TGATGAACATGATGGTCCAGTGCGAGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGA GATGACTATAAGATTAAGGTTTGGAATTACAAGCTTCGGCGCTGTCTTTTCACATTGCTTGGGCACTTAG ATTATATTCGCACCACGTTTTTTCATCATGAATATCCCTGGATTCTGAGTGCCTCCGATGATCAGACCAT CCGAGTGTGGAATTGGCAATCTAGAACCTGTGTTTGTGTGTTAACAGGGCACAACCATTATGTGATGTGT GCTCAGTTCCACCCCACAGAAGACTTGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGCGTTTGGGATA TTTCTGGTCTGAGGAAAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGATGTGAGAGGAATAACTGGGGT TGATCTATTTGGAACTACAGATGCAGTGGTGAAGCATGTACTAGAGGGTCACGATCGTGGAGTAAACTGG GCTGCCTTCCACCCCACTATGCCCCTTATTGTATCTGGGGCAGATGATCGTCAAGTGAAGATCTGGCGCA TGAATGAATCAAAGGCATGGGAGGTTGATACCTGCCGGGGCCATTACAACAATGTATCTTGTGCCGTCTT CCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG CGGACTGGGGTTCAGACTTTCCGCAGAGACCATGATCGTTTCTGGGTCCTAGCTGCTCACCCTAACCTTA ACCTCTTTGCAGCAGGCCATGATGGTGGTATGATTGTGTTTAAGCTGGAACGGGAACGGCCAGCCTATGC TGTTCATGGCAATATGCTACACTATGTCAAGGACCGATTCTTACGACAGCTGGATTTCAACAGCTCCAAA GATGTAGCTGTGATGCAGTTGCGGAGTGGTTCCAAGTTTCCAGTATTCAATATGTCATACAATCCAGCAG AAAATGCAGTCCTGCTTTGTACAAGAGCTAGCAATCTAGAGAATAGTACCTATGACCTGTACACCATCCC TAAAGATGCTGACTCCCAGAATCCTGATGCGCCTGAAGGGAAACGATCCTCAGGCCTGACAGCCGTTTGG GTCGCTCGAAATCGGTTTGCTGTCCTAGATCGGATGCATTCGCTTCTGATCAAGAATCTGAAGAATGAGA TCACCAAAAAGGTACAGGTGCCCAACTGTGATGAGATCTTCTATGCTGGCACAGGCAATCTCCTGCTTCG AGATGCGGACTCTATCACACTCTTTGACGTACAGCAGAAGCGGACTCTGGCATCTGTGAAGATTTCTAAA GTGAAATACGTTATCTGGTCAGCAGACATGTCACATGTAGCACTACTAGCCAAACACGAACACTCATGCC CTTTGCCTCTTACAGCCATTGTGATCTGTAACCGCAAACTGGATGCTTTATGTAACATTCATGAGAACAT TCGTGTCAAGAGTGGGGCCTGGGATGAGAGTGGGGTATTTATCTATACCACAAGCAACCACATCAAATAT GCTGTCACCACTGGGGACCACGGGATCATTCGAACTCTGGATTTACCCATCTATGTCACACGGGTGAAGG GCAACAATGTATACTGCCTAGACAGGGAGTGTCGTCCCCGGGTACTCACCATTGATCCCACTGAGTTCAA ATTCAAGCTGGCCCTGATCAACAGAAAATATGATGAGGTACTGCACATGGTGAGGAATGCCAAACTAGTT GGCCAGTCTATTATTGCTTATCTCCAGAAGAAGGGCTATCCTGAAGTGGCACTGCATTTTGTCAAGGATG AGAAAACTCGCTTTAGTCTGGCACTGGAGTGTGGAAACATTGAGATTGCTCTGGAAGCAGCCAAAGCACT GGATGACAAGAACTGCTGGGAAAAGCTGGGAGAAGTGGCCCTGCTGCAGGGGAACCACCAGATTGTGGAA ATGTGCTATCAGCGTACCAAAAACTTTGACAAACTTTCCTTCCTGTATCTTATCACTGGCAACTTAGAAA AACTTCGCAAGATGATGAAGATTGCTGAGATCAGAAAGGACATGAGTGGCCACTATCAGAATGCCCTATA CCTGGGTGATGTGTCAGAGCGTGTGCGGATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTCACA GCTGCTACCCATGGCTTAGATGAAGAAGCTGAGAGCCTAAAGGAGACATTTGACCCAGAGAAGGAGACAA TCCCAGACATTGACCCTAATGCCAAGCTGCTCCAGCCACCTGCACCTATCATGCCATTGGATACCAATTG GCCTTTATTGACTGTATCCAAAGGATTTTTTGAAGGCACCATTGCCAGCAAAGGGAAGGGAGGAGCACTG GCTGCTGACATTGACATTGACACTGTTGGTACAGAGGGCTGGGGAGAGGATGCAGAGCTGCAGTTGGATG AAGATGGGTTTGTGGAGGCTACAGAAGGTTTGGGGGATGATGCTCTTGGCAAGGGACAGGAAGAAGGAGG TGGCTGGGATGTAGAAGAAGATCTGGAGCTCCCTCCTGAGCTGGATATATCCCCTGGGGCAGCTGGTGGG GCTGAAGATGGTTTCTTTGTGCCCCCAACCAAGGGAACAAGTCCAACTCAGATCTGGTGTAATAACTCTC AGCTTCCAGTTGATCACATCCTGGCAGGCTCTTTCGAAACAGCCATGCGGCTCCTTCATGACCAAGTAGG GGTAATCCAGTTTGGCCCCTACAAGCAACTGTTCCTACAGACATACGCCCGAGGCCGCACAACCTATCAG GCTCTGCCCTGCCTACCCTCCATGTATGGCTATCCTAATCGCAACTGGAAGGATGCAGGGCTGAAGAATG GTGTACCAGCTGTGGGCCTGAAGCTTAATGACCTCATCCAACGGTTGCAGCTGTGCTACCAGCTCACCAC AGTTGGCAAATTTGAGGAGGCTGTGGAAAAATTCCGTTCCATCCTTCTCAGTGTGCCACTTCTTGTTGTG GACAATAAACAAGAGATTGCAGAGGCCCAGCAGCTCATCACCATTTGCCGTGAGTACATTGTGGGTTTGT CCGTGGAGACAGAAAGGAAGAAGCTGCCCAAAGAGACTCTAGAACAGCAGAAGCGCATCTGTGAGATGGC AGCCTATTTCACCCACTCAAACCTGCAGCCTGTGCACATGATCCTGGTGCTGCGTACAGCCCTCAATCTG TTCTTCAAGCTCAAGAACTTCAAGACAGCTGCCACCTTTGCTCGGCGCCTACTAGAACTCGGGCCCAAGC CTGAGGTGGCCCAACAGACCCGAAAAATCCTGTCTGCCTGTGAGAAGAATCCCACAGATGCCTACCAGCT CAATTATGACATGCACAACCCCTTTGACATTTGTGCTGCATCATATCGGCCCATCTACCGTGGAAAGCCA GTAGAAAAGTGTCCACTCAGTGGGGCCTGCTATTCCCCTGAGTTCAAAGGTCAAATCTGCAGGGTCACCA CAGTGACAGAGATTGGCAAAGATGTGATTGGTTTAAGGATCAGTCCTCTGCAGTTTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214199 representing NM_001098398
Red=Cloning site Green=Tags(s) MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGG DDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMC AQFHPTEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNW AAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSK RTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSK DVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVW VARNRFAVLDRMHSLLIKNLKNEITKKVQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISK VKYVIWSADMSHVALLAKHEHSCPLPLTAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKY AVTTGDHGIIRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLV GQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVE MCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLT AATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGTIASKGKGGAL AADIDIDTVGTEGWGEDAELQLDEDGFVEATEGLGDDALGKGQEEGGGWDVEEDLELPPELDISPGAAGG AEDGFFVPPTKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQ ALPCLPSMYGYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVV DNKQEIAEAQQLITICREYIVGLSVETERKKLPKETLEQQKRICEMAAYFTHSNLQPVHMILVLRTALNL FFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKP VEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098398 |
| ORF Size | 3699 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001098398.1, NP_001091868.1 |
| RefSeq Size | 5666 bp |
| RefSeq ORF | 3702 bp |
| Locus ID | 1314 |
| UniProt ID | P53621 |
| MW | 139.1 kDa |
| Gene Summary | In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Deficiency in coatomer complex I causes aberrant activation of STING signalling
,null,
Nature Communications
,PubMed ID 35484149
[COPA]
|
|
Mutations in COPA lead to abnormal trafficking of STING to the Golgi and interferon signaling
,Lepelley, A;Martin-Niclós, MJ;Le Bihan, M;Marsh, JA;Uggenti, C;Rice, GI;Bondet, V;Duffy, D;Hertzog, J;Rehwinkel, J;Amselem, S;Boulisfane-El Khalifi, S;Brennan, M;Carter, E;Chatenoud, L;Chhun, S;Coulomb l'Hermine, A;Depp, M;Legendre, M;Mackenzie, KJ;Marey, J;McDougall, C;McKenzie, KJ;Molina, TJ;Neven, B;Seabra, L;Thumerelle, C;Wislez, M;Nathan, N;Manel, N;Crow, YJ;Frémond, ML;,
J. Exp. Med.
,PubMed ID 32725128
[COPA]
|
|
Activation of STING due to COPI-deficiency
,Steiner, A;Schaale, K;Prigione, I;De Nardo, D;Dagley, L;Yu, C;Laohamonthonkul, P;Harapas, C;Gantier, M;Gattorno, M;Volpi, S;Davidson, S;Masters, S;,
bioRxiv
[COPA]
|
|
COPA mutations impair ER-Golgi transport and cause hereditary autoimmune-mediated lung disease and arthritis
,Watkin, LB;Jessen, B;Wiszniewski, W;Vece, TJ;Jan, M;Sha, Y;Thamsen, M;Santos-Cortez, RL;Lee, K;Gambin, T;Forbes, LR;Law, CS;Stray-Pedersen, A;Cheng, MH;Mace, EM;Anderson, MS;Liu, D;Tang, LF;Nicholas, SK;Nahmod, K;Makedonas, G;Canter, DL;Kwok, PY;Hicks, J;Jones, KD;Penney, S;Jhangiani, SN;Rosenblum, MD;Dell, SD;Waterfield, MR;Papa, FR;Muzny, DM;Zaitlen, N;Leal, SM;Gonzaga-Jauregui, C;Baylor-Hopkins Center for Mendelian Genomics, ;Boerwinkle, E;Eissa, NT;Gibbs, RA;Lupski, JR;Orange, JS;Shum, AK;,
Nat. Genet.
,PubMed ID 25894502
[COPA]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214199L1 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, Myc-DDK-tagged |
CNY 15,280.00 |
|
| RC214199L2 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, mGFP tagged |
CNY 15,280.00 |
|
| RC214199L3 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, Myc-DDK-tagged |
CNY 14,160.00 |
|
| RC214199L4 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, mGFP tagged |
CNY 14,160.00 |
|
| RG214199 | COPA (tGFP-tagged) - Human coatomer protein complex, subunit alpha (COPA), transcript variant 1 |
CNY 14,480.00 |
|
| SC316290 | COPA (untagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1 |
CNY 12,888.00 |
