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Acyl coenzyme A Thioesterase 8 (ACOT8) (NM_005469) Human Tagged ORF Clone
CAT＃: RC214175
ACOT8 (Myc-DDK-tagged)-Human acyl-CoA thioesterase 8 (ACOT8)
ORF Plasmid: tGFP
"NM_005469" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hACTE-III; HNAACTE; hTE; NAP1; PTE-1; PTE-2; PTE1; PTE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214175 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCCCGCAGGCCCCAGAAGATGGGCAGGGCTGTGGCGACCGCGGCGATCCCCCTGGGGACCTCC GTAGCGTCTTGGTCACGACCGTGCTCAACCTCGAGCCGCTGGACGAGGATCTCTTCAGAGGAAGGCATTA CTGGGTACCGGCCAAGAGGCTGTTTGGTGGTCAGATCGTGGGCCAGGCCCTGGTGGCTGCAGCCAAGTCT GTGAGTGAAGACGTCCACGTGCACTCCCTGCACTGCTACTTTGTTCGGGCAGGGGACCCGAAGCTGCCAG TACTGTACCAAGTGGAGCGGACACGAACAGGGTCGAGCTTCTCGGTGCGCTCTGTGAAGGCCGTGCAACA TGGGAAGCCCATCTTCATCTGCCAGGCCTCCTTCCAGCAGGCCCAGCCCAGCCCCATGCAGCACCAGTTC TCCATGCCCACTGTGCCACCACCAGAAGAGCTGCTTGACTGTGAGACCCTCATTGACCAGTATTTAAGGG ACCCTAACCTCCAAAAGAGGTACCCATTGGCGCTCAACCGAATTGCTGCTCAGGAGGTCCCCATTGAGAT CAAGCCAGTAAACCCATCCCCCCTGAGCCAGCTGCAGAGAATGGAGCCCAAACAGATGTTCTGGGTGCGA GCCCGGGGCTATATTGGCGAGGGCGACATGAAGATGCACTGCTGCGTGGCCGCCTATATCTCCGACTATG CCTTCTTGGGCACTGCACTGCTGCCTCACCAGTGGCAGCACAAGGTGCACTTCATGGTCTCACTGGACCA TTCCATGTGGTTCCACGCCCCCTTCCGAGCTGACCACTGGATGCTCTATGAATGCGAGAGCCCCTGGGCC GGTGGCTCTCGGGGGCTGGTCCATGGGCGGCTGTGGCGTCAGGATGGAGTCCTAGCTGTGACCTGTGCCC AGGAGGGCGTGATCCGAGTGAAGCCCCAGGTCTCAGAGAGCAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214175 protein sequence
Red=Cloning site Green=Tags(s) MSSPQAPEDGQGCGDRGDPPGDLRSVLVTTVLNLEPLDEDLFRGRHYWVPAKRLFGGQIVGQALVAAAKS VSEDVHVHSLHCYFVRAGDPKLPVLYQVERTRTGSSFSVRSVKAVQHGKPIFICQASFQQAQPSPMQHQF SMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVR ARGYIGEGDMKMHCCVAAYISDYAFLGTALLPHQWQHKVHFMVSLDHSMWFHAPFRADHWMLYECESPWA GGSRGLVHGRLWRQDGVLAVTCAQEGVIRVKPQVSESKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005469 |
| ORF Size | 957 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005469.4 |
| RefSeq Size | 1188 bp |
| RefSeq ORF | 960 bp |
| Locus ID | 10005 |
| UniProt ID | O14734 |
| Domains | Acyl_CoA_thio |
| MW | 35.9 kDa |
| Gene Summary | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214175L3 | Lenti ORF clone of Human acyl-CoA thioesterase 8 (ACOT8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214175L4 | Lenti ORF clone of Human acyl-CoA thioesterase 8 (ACOT8), mGFP tagged |
CNY 5,890.00 |
|
| RG214175 | ACOT8 (tGFP-tagged) - Human acyl-CoA thioesterase 8 (ACOT8) |
CNY 4,000.00 |
|
| SC111742 | ACOT8 (untagged)-Human acyl-CoA thioesterase 8 (ACOT8) |
CNY 2,400.00 |
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