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Bestrophin (BEST1) (NM_004183) Human Tagged ORF Clone
CAT＃: RC214156
BEST1 (Myc-DDK-tagged)-Human bestrophin 1 (BEST1), transcript variant 1
ORF Plasmid: tGFP
"NM_004183" in other vectors (6)
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CNY 6,536.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARB; BEST; Best1V1Delta2; BMD; RP50; TU15B; VMD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214156 representing NM_004183
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATCACTTACACAAGCCAAGTGGCTAATGCCCGCTTAGGCTCCTTCTCCCGCCTGCTGCTGTGCT GGCGGGGCAGCATCTACAAGCTGCTATATGGCGAGTTCTTAATCTTCCTGCTCTGCTACTACATCATCCG CTTTATTTATAGGCTGGCCCTCACGGAAGAACAACAGCTGATGTTTGAGAAACTGACTCTGTATTGCGAC AGCTACATCCAGCTCATCCCCATTTCCTTCGTGCTGGGCTTCTACGTGACGCTGGTCGTGACCCGCTGGT GGAACCAGTACGAGAACCTGCCGTGGCCCGACCGCCTCATGAGCCTGGTGTCGGGCTTCGTCGAAGGCAA GGACGAGCAAGGCCGGCTGCTGCGGCGCACGCTCATCCGCTACGCCAACCTGGGCAACGTGCTCATCCTG CGCAGCGTCAGCACCGCAGTCTACAAGCGCTTCCCCAGCGCCCAGCACCTGGTGCAAGCAGGCTTTATGA CTCCGGCAGAACACAAGCAGTTGGAGAAACTGAGCCTACCACACAACATGTTCTGGGTGCCCTGGGTGTG GTTTGCCAACCTGTCAATGAAGGCGTGGCTTGGAGGTCGAATCCGGGACCCTATCCTGCTCCAGAGCCTG CTGAACGAGATGAACACCTTGCGTACTCAGTGTGGACACCTGTATGCCTACGACTGGATTAGTATCCCAC TGGTGTATACACAGGTGGTGACTGTGGCGGTGTACAGCTTCTTCCTGACTTGTCTAGTTGGGCGGCAGTT TCTGAACCCAGCCAAGGCCTACCCTGGCCATGAGCTGGACCTCGTTGTGCCCGTCTTCACGTTCCTGCAG TTCTTCTTCTATGTTGGCTGGCTGAAGGTGGCAGAGCAGCTCATCAACCCCTTTGGAGAGGATGATGATG ATTTTGAGACCAACTGGATTGTCGACAGGAATTTGCAGGTGTCCCTGTTGGCTGTGGATGAGATGCACCA GGACCTGCCTCGGATGGAGCCGGACATGTACTGGAATAAGCCCGAGCCACAGCCCCCCTACACAGCTGCT TCCGCCCAGTTCCGTCGAGCCTCCTTTATGGGCTCCACCTTCAACATCAGCCTGAACAAAGAGGAGATGG AGTTCCAGCCCAATCAGGAGGACGAGGAGGATGCTCACGCTGGCATCATTGGCCGCTTCCTAGGCCTGCA GTCCCATGATCACCATCCTCCCAGGGCAAACTCAAGGACCAAACTACTGTGGCCCAAGAGGGAATCCCTT CTCCACGAGGGCCTGCCCAAAAACCACAAGGCAGCCAAACAGAACGTTAGGGGCCAGGAAGACAACAAGG CCTGGAAGCTTAAGGCTGTGGACGCCTTCAAGTCTGCCCCACTGTATCAGAGGCCAGGCTACTACAGTGC CCCACAGACGCCCCTCAGCCCCACTCCCATGTTCTTCCCCCTAGAACCATCAGCGCCGTCAAAGCTTCAC AGTGTCACAGGCATAGACACCAAAGACAAAAGCTTAAAGACTGTGAGTTCTGGGGCCAAGAAAAGTTTTG AATTGCTCTCAGAGAGCGATGGGGCCTTGATGGAGCACCCAGAAGTATCTCAAGTGAGGAGGAAAACTGT GGAGTTTAACCTGACGGATATGCCAGAGATCCCCGAAAATCACCTCAAAGAACCTTTGGAACAATCACCA ACCAACATACACACTACACTCAAAGATCACATGGATCCTTATTGGGCCTTGGAAAACAGGGATGAAGCAC ATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214156 representing NM_004183
Red=Cloning site Green=Tags(s) MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCD SYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLIL RSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSL LNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQ FFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAA SAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESL LHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLH SVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSP TNIHTTLKDHMDPYWALENRDEAHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004183 |
| ORF Size | 1755 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004183.4 |
| RefSeq Size | 2673 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 7439 |
| UniProt ID | O76090 |
| Domains | DUF289 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 67.5 kDa |
| Gene Summary | This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Effects of alternative splicing on function of Bestrophin-1 calcium-activated chloride channels
,null,
The Biochemical journal
,PubMed ID 24341532
[Bestrophin]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214156L1 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 1, Myc-DDK-tagged |
CNY 8,936.00 |
|
| RC214156L2 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC214156L3 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214156L4 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG214156 | BEST1 (tGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 1 |
CNY 5,040.00 |
|
| SC310242 | BEST1 (untagged)-Human bestrophin 1 (BEST1), transcript variant 1 |
CNY 6,544.00 |
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