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PIK3AP1 (NM_152309) Human Tagged ORF Clone
CAT＃: RC214125
PIK3AP1 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1)
ORF Plasmid: tGFP
"NM_152309" in other vectors (6)
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CNY 5,896.00
CNY 8,460.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214125 representing NM_152309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTCAGGGGTGCCCAGAGGATGCGACATCCTCATCGTCTACAGCCCGGATGCCGAGGAATGGT GCCAGTACCTGCAGACCCTGTTCCTGTCCAGTCGGCAGGTCCGCAGCCAGAAGATACTGACTCACAGGCT GGGCCCCGAGGCCTCCTTCTCGGCAGAGGACCTAAGCCTTTTCCTCAGCACCCGCTGTGTCGTGGTGCTG CTGTCCGCGGAGCTGGTGCAGCACTTCCACAAGCCCTCCTTGCTGCCCCTGCTGCAGAGAGCTTTCCATC CTCCGCACCGCGTGGTCAGGCTGCTCTGCGGCGTGCGGGACAGCGAGGAGTTCCTAGACTTCTTTCCAGA TTGGGCCCATTGGCAGGAGCTCACCTGTGACGATGAGCCAGAGACCTACGTGGCAGCTGTGAAAAAAGCC ATTTCCGAAGATTCTGGCTGTGACTCAGTCACTGACACTGAGCCTGAGGACGAGAAGGTTGTTTCCTACT CGAAGCAGCAGAACCTGCCGACGGTGACTTCACCTGGGAACCTGATGGTGGTGCAGCCGGACCGCATTCG CTGTGGGGCAGAAACCACTGTCTATGTTATTGTGAGATGTAAGCTGGATGACAGGGTGGCGACAGAAGCA GAGTTTTCTCCTGAGGATTCTCCCTCTGTAAGGATGGAAGCCAAGGTGGAGAATGAGTACACCATTTCAG TGAAGGCTCCCAACCTTTCATCTGGGAACGTTTCTCTGAAGATATATTCTGGAGACTTAGTGGTGTGTGA AACCGTTATCAGCTATTATACTGACATGGAAGAAATTGGGAATTTATTGTCCAATGCCGCGAATCCTGTG GAATTCATGTGTCAGGCCTTTAAAATTGTGCCCTACAACACAGAGACCCTTGATAAACTGCTAACCGAAT CCCTGAAGAACAATATCCCTGCAAGCGGACTGCACCTCTTTGGAATCAACCAGCTGGAAGAAGAAGATAT GATGACAAATCAGAGGGATGAAGAGCTGCCCACCCTGTTGCATTTTGCTGCGAAGTATGGACTGAAGAAC CTCACTGCCTTGTTGCTCACCTGCCCAGGAGCCCTGCAGGCGTACAGCGTGGCCAACAAGCATGGCCACT ACCCCAACACCATCGCTGAGAAACACGGCTTCAGGGACCTGCGGCAGTTCATCGACGAGTATGTGGAAAC GGTGGACATGCTCAAGAGTCACATTAAAGAGGAACTGATGCACGGGGAGGAGGCTGATGCTGTGTACGAG TCCATGGCCCACCTTTCCACAGACCTGCTTATGAAATGCTCGCTCAACCCCGGCTGTGACGAGGATCTCT ATGAGTCCATGGCTGCCTTTGTCCCAGCTGCCACTGAAGACCTCTATGTTGAAATGCTTCAGGCCAGTAC ATCTAACCCAATCCCTGGAGATGGTTTCTCTCGGGCCACTAAGGACTCTATGATCCGCAAGTTTTTAGAA GGCAACAGCATGGGAATGACCAATCTGGAGAGAGATCAGTGCCATCTTGGTCAGGAAGAAGATGTTTATC ACACGGTGGATGACGATGAGGCCTTTTCTGTGGACTTGGCCAGCAGGCCCCCTGTCCCAGTGCCCAGACC AGAGACCACTGCTCCTGGTGCTCACCAGCTGCCTGACAACGAACCATACATTTTTAAAGTTTTTGCAGAA AAAAGTCAAGAGCGGCCTGGGAATTTCTACGTTTCCTCAGAGAGCATCAGGAAAGGGCCGCCCGTCAGAC CATGGAGGGACAGGCCCCAGTCAAGTATATATGACCCTTTTGCGGGAATGAAAACGCCAGGCCAGCGGCA GCTTATCACCCTCCAGGAGCAGGTGAAGCTGGGCATTGTCAACGTGGATGAGGCTGTGCTCCACTTCAAA GAGTGGCAGCTCAACCAGAAGAAACGATCGGAGTCCTTTCGTTTCCAGCAGGAAAATCTTAAACGGCTAA GAGACAGCATCACCCGAAGACAGAGAGAGAAGCAAAAATCAGGAAAGCAGACAGACTTGGAGATCACGGT CCCAATTCGGCACTCACAGCACCTGCCTGCAAAAGTGGAGTTTGGAGTCTATGAGAGTGGCCCCAGGAAA AGTGTCATTCCCCCTAGGACGGAGCTGAGACGAGGAGACTGGAAAACAGACAGCACCTCCAGCACAGCAA GTAGCACAAGTAACCGCTCCAGCACCCGGAGCCTCCTCAGTGTGAGCAGCGGGATGGAAGGGGACAACGA GGATAATGAAGTCCCTGAGGTTACCAGAAGTCGCAGTCCAGGCCCCCCACAAGTGGATGGGACACCCACC ATGTCCCTCGAGAGACCCCCCAGGGTGCCTCCGAGAGCTGCCTCACAGAGGCCTCCGACCAGGGAGACCT TCCATCCTCCTCCACCTGTTCCACCCAGAGGACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214125 representing NM_152309
Red=Cloning site Green=Tags(s) MAASGVPRGCDILIVYSPDAEEWCQYLQTLFLSSRQVRSQKILTHRLGPEASFSAEDLSLFLSTRCVVVL LSAELVQHFHKPSLLPLLQRAFHPPHRVVRLLCGVRDSEEFLDFFPDWAHWQELTCDDEPETYVAAVKKA ISEDSGCDSVTDTEPEDEKVVSYSKQQNLPTVTSPGNLMVVQPDRIRCGAETTVYVIVRCKLDDRVATEA EFSPEDSPSVRMEAKVENEYTISVKAPNLSSGNVSLKIYSGDLVVCETVISYYTDMEEIGNLLSNAANPV EFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEEDMMTNQRDEELPTLLHFAAKYGLKN LTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKSHIKEELMHGEEADAVYE SMAHLSTDLLMKCSLNPGCDEDLYESMAAFVPAATEDLYVEMLQASTSNPIPGDGFSRATKDSMIRKFLE GNSMGMTNLERDQCHLGQEEDVYHTVDDDEAFSVDLASRPPVPVPRPETTAPGAHQLPDNEPYIFKVFAE KSQERPGNFYVSSESIRKGPPVRPWRDRPQSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFK EWQLNQKKRSESFRFQQENLKRLRDSITRRQREKQKSGKQTDLEITVPIRHSQHLPAKVEFGVYESGPRK SVIPPRTELRRGDWKTDSTSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEVPEVTRSRSPGPPQVDGTPT MSLERPPRVPPRAASQRPPTRETFHPPPPVPPRGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152309 |
| ORF Size | 2415 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152309.2, NP_689522.2 |
| RefSeq Size | 4817 bp |
| RefSeq ORF | 2418 bp |
| Locus ID | 118788 |
| UniProt ID | Q6ZUJ8 |
| Protein Families | Druggable Genome |
| Protein Pathways | B cell receptor signaling pathway |
| MW | 90.2 kDa |
| Gene Summary | Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214125L1 | Lenti ORF clone of Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), Myc-DDK-tagged |
CNY 8,296.00 |
|
| RC214125L2 | Lenti ORF clone of Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), mGFP tagged |
CNY 10,360.00 |
|
| RC214125L3 | Lenti ORF clone of Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), Myc-DDK-tagged |
CNY 10,360.00 |
|
| RC214125L4 | Lenti ORF clone of Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), mGFP tagged |
CNY 10,360.00 |
|
| RG214125 | PIK3AP1 (tGFP-tagged) - Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1) |
CNY 7,496.00 |
|
| SC100911 | PIK3AP1 (untagged)-Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1) |
CNY 5,904.00 |
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