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GALR1 (NM_001480) Human Tagged ORF Clone
CAT＃: RC213471
GALR1 (Myc-DDK-tagged)-Human galanin receptor 1 (GALR1)
ORF Plasmid: tGFP
"NM_001480" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GALNR; GALNR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGCGGTCGGGAACCTCAGCGAGGGCAACGCGAGCTGGCCGGAGCCCCCCGCCCCGGAGCCCG GGCCGCTGTTCGGCATCGGCGTGGAGAACTTCGTCACGCTGGTGGTGTTCGGCCTGATCTTCGCGCTGGG CGTGCTGGGCAACAGCCTAGTGATCACCGTGCTGGCGCGCAGCAAGCCGGGCAAGCCGCGGAGCACCACC AACCTGTTCATCCTCAACCTGAGCATCGCCGACCTGGCCTACCTGCTCTTCTGCATCCCCTTCCAGGCCA CCGTGTACGCGCTGCCCACCTGGGTGCTGGGCGCCTTCATCTGCAAGTTCATCCACTACTTCTTCACCGT GTCCATGCTGGTGAGCATCTTCACCCTGGCCGCGATGTCCGTGGACCGCTACGTGGCCATCGTGCACTCG CGGCGCTCCTCCTCCCTCAGGGTGTCCCGCAACGCGCTGCTGGGCGTGGGCTGCATCTGGGCGCTGTCCA TTGCCATGGCCTCGCCCGTGGCCTACCACCAGGGCCTCTTCCACCCGCGCGCCAGCAACCAGACCTTCTG CTGGGAGCAGTGGCCCGACCCTCGCCACAAGAAGGCCTACGTGGTGTGCACCTTCGTCTTCGGCTACCTG CTGCCGCTCCTGCTCATCTGCTTCTGCTATGCCAAGGTCCTTAATCACTTGCATAAAAAGTTGAAGAACA TGTCAAAGAAGTCTGAAGCATCCAAGAAAAAGACTGCACAGACAGTTCTGGTGGTGGTTGTGGTGTTTGG AATCTCCTGGCTGCCGCACCACATCATCCATCTCTGGGCTGAGTTTGGAGTTTTCCCGCTGACGCCGGCT TCCTTCCTCTTCAGAATCACCGCCCACTGCCTGGCGTACAGCAATTCCTCCGTGAATCCTATCATTTATG CATTTCTCTCTGAAAATTTCAGGAAGGCCTATAAACAAGTGTTCAAGTGTCACATTCGCAAAGATTCACA CCTGAGTGATACTAAAGAAAATAAAAGTCGAATAGACACCCCACCATCAACCAATTGTACTCATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213471 protein sequence
Red=Cloning site Green=Tags(s) MELAVGNLSEGNASWPEPPAPEPGPLFGIGVENFVTLVVFGLIFALGVLGNSLVITVLARSKPGKPRSTT NLFILNLSIADLAYLLFCIPFQATVYALPTWVLGAFICKFIHYFFTVSMLVSIFTLAAMSVDRYVAIVHS RRSSSLRVSRNALLGVGCIWALSIAMASPVAYHQGLFHPRASNQTFCWEQWPDPRHKKAYVVCTFVFGYL LPLLLICFCYAKVLNHLHKKLKNMSKKSEASKKKTAQTVLVVVVVFGISWLPHHIIHLWAEFGVFPLTPA SFLFRITAHCLAYSNSSVNPIIYAFLSENFRKAYKQVFKCHIRKDSHLSDTKENKSRIDTPPSTNCTHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001480 |
| ORF Size | 1047 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001480.2, NP_001471.1 |
| RefSeq Size | 2787 bp |
| RefSeq ORF | 1050 bp |
| Locus ID | 2587 |
| UniProt ID | P47211 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 39 kDa |
| Gene Summary | The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213471L3 | Lenti ORF clone of Human galanin receptor 1 (GALR1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213471L4 | Lenti ORF clone of Human galanin receptor 1 (GALR1), mGFP tagged |
CNY 5,890.00 |
|
| RG213471 | GALR1 (tGFP-tagged) - Human galanin receptor 1 (GALR1) |
CNY 5,256.00 |
|
| SC303029 | GALR1 (untagged)-Human galanin receptor 1 (GALR1) |
CNY 3,656.00 |
