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EMR2 (ADGRE2) (NM_013447) Human Tagged ORF Clone
CAT＃: RC213463
EMR2 (Myc-DDK-tagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1
ORF Plasmid: tGFP
"NM_013447" in other vectors (6)
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CNY 6,032.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD97; CD312; EMR2; VBU |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213463 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCC AGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTG CAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAG TGTGCAACACTGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACT GCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACGTG TCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTT GGCAGCTATACCTGCCAGTGCCTGCCTGGCTTCAAGTTCATACCTGAGGATCCGAAGGTCTGCACAGATG TGAATGAATGCACCTCCGGACAAAACCCATGCCACAGCTCCACCCACTGCCTCAACAACGTGGGCAGCTA TCAGTGCCGCTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGT GAAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGG GTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACAC TGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGA TTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCA TCTTACAGGCGCTGGATGAGCTGCTGGAGGTCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCA CTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAAT GGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGA GTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCC AGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTC CTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCC TGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTT CACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAAT GGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTT GCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGAC TGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTG TGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACC TCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCA CTATCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAAC CTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAG TCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTG GCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTA GTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCC TCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCT GGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAG GGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCA AAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTC CAAACCCAGCACGGTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213463 protein sequence
Red=Cloning site Green=Tags(s) MGGRVFLVFLAFCVWLTLPGAETQDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINE CATLSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTL GSYTCQCLPGFKFIPEDPKVCTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVC EDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSR FFDKVQDLGRDYKPGLANNTIQSILQALDELLEVPGDLETLPRLQQHCVASHLLDGLEDVLRGLSKNLSN GLLNFSYPAGTELSLEVQKQVDRSVTLRQNQAVMQLDWNQAQKSGDPGPSVVGLVSIPGMGKLLAEAPLV LEPEKQMLLHETHQGLLQDGSPILLSDVISAFLSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQN GCGHWATTGCSTIGTRDTSTICRCTHLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALTFLL CKAIQNTSTSLHLQLSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLLEALYLFLTARN LTVVNYSSINRFMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSRCWLQPEKGFIWGFLGPVCAIFSVNL VLFLVTLWILKNRLSSLNSEVSTLRNTRMLAFKATAQLFILGCTWCLGILQVGPAARVMAYLFTIINSLQ GVFIFLVYCLLSQQVREQYGKWSKGIRKLKTESEMHTLSSSAKADTSKPSTVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013447 |
| ORF Size | 2469 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013447.4 |
| RefSeq Size | 6483 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 30817 |
| UniProt ID | Q9UHX3 |
| Domains | GPS, 7tm_2, EGF_CA, EGF, EGF |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 90.5 kDa |
| Gene Summary | This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213463L1 | Lenti ORF clone of Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1, Myc-DDK-tagged |
CNY 8,432.00 |
|
| RC213463L2 | Lenti ORF clone of Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC213463L3 | Lenti ORF clone of Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213463L4 | Lenti ORF clone of Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG213463 | EMR2 (tGFP-tagged) - Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1 |
CNY 7,632.00 |
|
| SC115222 | EMR2 (untagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1 |
CNY 6,032.00 |
